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Citations for BioLegend :
551 - 600 of 6072 citations for Recombinant Human CD33 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... phagocytosis was stopped by washing with 4°C PBS and centrifugation at 1200 RPMI before the cells were stained with APC-labeled anti-CD11b (Clone M1/70, Biolegend) to identify human macrophages and Live/Dead stain (Fixable Viability Dye ...
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bioRxiv - Cancer Biology 2021Quote: ... allophycocyanin (APC)-Cy7 or APC-conjugated CD8 (BioLegend Cat# 100759 ...
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bioRxiv - Immunology 2022Quote: ... APC Cy7/APC Fire750-conjugated: CD8α (Biolegend, 344746), CCR7 (CD197 ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were treated with 200 ng/mL recombinant human NRG1 (BioLegend, Cat. No. 551904) for 48 hrs ...
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bioRxiv - Immunology 2024Quote: ... The ultrasound-guided intra-amniotic injection of recombinant human HMGB1 (Biolegend, San Diego, CA, USA) at a concentration of 9 ng dissolved in 100 μL of sterile 1X phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... 550 µl of RPMI medium supplemented with 1 µg/ml recombinant human CCL21 (BioLegend; #582208) or nothing as negative control were added to the lower chambers ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Genomics 2020Quote: ... Fc receptors were blocked by incubating the cells with 50 μl Human TruStain FcX (Biolegend, #422302) for 10 minutes at room temperature prior to antibody staining and sorting ...
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bioRxiv - Immunology 2022Quote: ... Whole blood cell staining was performed using an Fc receptor blocking solution (Human TruStain FcX; BioLegend), the viability dye eFluor780 (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Cells were pelleted again and resuspended in 100 ul of Fc-Block (Human TruStain FcX, Biolegend), and 10 ul aliquot was taken for counting using K2 Cellometer (Nexcelom ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Microbiology 2022Quote: ... blocked with Fc Block for 20 min at room temperature (Human TruStain FcX, Biolegend Cat# 422302), and simultaneously stained for surface antigenic Site Ø ...
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bioRxiv - Immunology 2023Quote: Zombie Aqua Fixable Viability Dye and Human TruStain FcX Fc Receptor Blocking Solution were from BioLegend. CF700-SE and CF750-SE were bought from Biotium ...
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bioRxiv - Bioengineering 2024Quote: The scFc protein was labeled with SNAP-Surface 488 (BG-488, Biolegend). In brief ...
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bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... Red blood cell lysis was performed and resulting leukocytes were resuspended in PBS + 1% BSA and blocked for 10 mins on ice with FC block and stained with Sytox® blue viability marker and conjugated antibodies anti-CD45-APC/Cy7 (Biolegend, 103115), anti-CD11b-APC (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Recombinant mouse (rm) M-CSF and GM-CSF proteins were obtained from BioLegend. MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Cancer Biology 2022Quote: Flow cytometry analysis or flow cytometry cell sorting was performed using anti-human CD133-APC (BioLegend – 372805) and anti-human CD44-APC (BioLegend – 338805 ...
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bioRxiv - Cancer Biology 2019Quote: ... TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6) (BioLegend, CA) and FITC anti-human KRAS antibody (Catalog# ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... 0.5 mL 200 mg/mL APC/Cy7-mouse-anti-human CD3 (BioLegend; Cat. No. 317342; clone OKT3), 2 mL 200 mg/mL BV711-mouse-anti-human CD49a (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Later 25µl of the following mixture of fluorescent-labeled antibodies was added to the samples: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed and stained with an Allophycocyanin (APC) labeled secondary antibody (Donkey anti-rabbit IgG DyLight™ 649, Biolegend 406406) for 30-45 minutes at room temperature in 2% normal donkey serum ...
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bioRxiv - Immunology 2019Quote: ... CD8a APC-Cyanine 7 (APC-Cy7, clone: HIT8a, Biolegend), PD-1 phycoerythrin (PE)-Cy7 (clone EH12.2H7 ...
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bioRxiv - Immunology 2019Quote: ... Sections were labeled overnight at 4°C with mouse anti-human CD1c-PE (L161, Biolegend) or mouse anti-human Clec9A-PE (8F9 ...
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bioRxiv - Immunology 2022Quote: ... Nalm6 and primary B cells were labeled with PE/Cy7 anti-human CD19 Antibody (Biolegend) and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: ... such as the recombinant human matrix metalloproteinases 12 (MMP12, 1 μl, 20 ng/ml, Biolegend, U.S.A), MMP408 (the inhibitor of MMP12 ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Bio-PyCSP protein (38.3 kDa) at 16 μM was combined with SA-APC antibody (Biolegend) at 2 μM ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in MACS buffer and incubated with PE–anti-human FC antibody (Biolegend, M1310G05) in MACS buffer for 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used for flow cytometry staining: APC/Cy7 anti-human CD3 Antibody (clone HIT3a, Biolegend), Brilliant Violet 605™ anti-human CD56 (NCAM ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with a mouse anti-human C3 APC conjugated antibody (1:50; Biolegend, 846106, clone 3E7iC3b) and mouse anti-Myc Alexa488 conjugated antibody (1:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... Blocking buffer was then removed and cells were incubated with an APC-conjugated anti-human EpCAM (BioLegend, 369809) antibody diluted 1:20 in dilution buffer (1x PBS-T ...
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bioRxiv - Bioengineering 2022Quote: ... Enriched CD34+ HSPCs were stained with APC anti-human CD34 antibodies (clone 581, Biolegend, San Jose, CA, USA), and sample purity was assessed on an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Macrophages were instead stained with 5 ul of either APC/Cy7- anti human CD16 antibody clone 3G8 (Biolegend), APC-anti human CD206 antibody (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.