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Citations for BioLegend :
651 - 700 of 2924 citations for Recombinant Human CD274 His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Immunology 2019Quote: ... OVA-specific IgG1 was detected using biotinylated anti-mouse IgG1 monoclonal antibody (0.02µg/mL RMG1-1, Biolegend – CA, USA) followed by SA-HRP (1:2000 dilution #P030701-2 ...
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bioRxiv - Immunology 2021Quote: ... PD-1 tetramers were prepared by incubating a 5:1 ratio of biotinylated PD-1 and PE streptavidin (BioLegend) for 10 minutes on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens: cKit (2B8, Biolegend), Sca1 (D7 ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend).
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bioRxiv - Immunology 2022Quote: ... enriched single-cell suspensions from tumor lysates were labelled with biotinylated anti-CD11b antibody (BioLegend, San Diego, CA, USA) and separated on MACS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and biotinylated isotype controls (Rat IgG2a,κ clone RTK2758 and Rat IgG2b,κ clone RTK4530) were purchased from Biolegend. Biotinylated antiPD-1 antibody (clone J43 ...
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bioRxiv - Immunology 2023Quote: ... a 10X molar excess of biotinylated RBD-Avi protein was incubated with 0.5 mg/ml of streptavidin-AlexaFluor 488 or streptavidin-AlexaFluor 647 (BioLegend) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... fibroblasts and in vitro differentiated plasmablasts (generally <10% frequency) were removed using biotinylated anti-H-2Kd (Biolegend, cat: 116604) and anti-CD138 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... IFNγ ELISAs used the MD-1 clone for coating and a biotinylated 4S.B3 clone for detection prior to visualization with a streptavidin-HRP antibody (BioLegend).
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bioRxiv - Immunology 2024Quote: ... Truncated EGFR expression was detected using biotinylated cetuximab (Bristol-Myers Squibb) and a phycoerythrin (PE)-conjugated streptavidin antibody (BioLegend). T cell activation was assessed by fluorophore-conjugated monoclonal antibodies detecting CD137 (also known as TNFRSF9 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting for GFP-positive CD298+MHC-I− PDX cells with fluorescently tagged antibodies for CD298 (Biolegend, cat. no. 341704) and MHC-I (eBioscience ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Immunology 2022Quote: ... then incubated at 37 °C in the presence or absence of recombinant murine CCL28 (BioLegend) at the indicated concentrations ...
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bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Developmental Biology 2023Quote: ... one uterine horn of XER mice was injected intraluminally with 1µg recombinant Lif (BioLegend, 554006) at 1300h on GD3 ...
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bioRxiv - Immunology 2024Quote: ... BMDCs were generated by cultivating BM cells supplemented with recombinant mouse GM-CSF (#576308, BioLegend) or recombinant mouse Flt3L (550706 ...
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bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 1 hour with mouse HRP α-His Tag antibody (1:1000 in 5% milk) (BioLegend, USA) and developed ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human CD45-APC (Biolegend, 368512). Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... anti-human CD34-Alexa 488 (Biolegend, 343518) and anti-human CD235a-APC (Ebioscience ...
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bioRxiv - Cell Biology 2022Quote: ... anti-human CD3-Pacific Blue (Biolegend, 344823), anti-human CD19-PECy7 (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... using the LEGENDplex Human Angiogenesis Panel (BioLegend) bead-based immunoassay ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... PE anti-mouse/human CD11b Antibody (Biolegend, Cat# 101208 ...
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bioRxiv - Immunology 2021Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-human CD3 antibody (Biolegend, 300306) and PE streptavidin (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... and mouse anti-human CD45 (HI30, Biolegend) overnight followed by 1hr incubation at room temperature with goat anti-mouse Cy3 (Jackson laboratory ...
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bioRxiv - Synthetic Biology 2019Quote: ... and mouse anti human CD4 APC (Biolegend),
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bioRxiv - Cancer Biology 2019Quote: ... and APC anti-human CD4 (Biolegend 300514). 68% of sgCD45-transduced cells expressed CD45 and 28% of sgCD4-transduced cells expressed CD4.
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bioRxiv - Immunology 2019Quote: ... blocked with human TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Immunology 2020Quote: ... APC/Cy7 anti-human CD3 (HIT3a, Biolegend), Brilliant Violet 605™ anti-human CD56 (NCAM ...
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bioRxiv - Immunology 2020Quote: ... APC/Cy7 anti-human CD3 (HIT3a, Biolegend), Brilliant Violet 605™ anti-human CD56 (NCAM ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human-CD8-PE/Dazzle-594 (BioLegend), and anti-human CD69 APC (BioLegend).
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD4 Brilliant Violet 786 (BioLegend), anti-human-CD8-PE/Dazzle-594 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and FITC anti-human Granzyme B (BioLegend) staining.
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bioRxiv - Cancer Biology 2021Quote: ... mouse/human CD44-FITC (IM7, Biolegend 103005), FC ...
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bioRxiv - Bioengineering 2020Quote: ... and antibodies to human CD3 (HIT3a, BioLegend), CD45 (2D1 ...
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bioRxiv - Bioengineering 2021Quote: ... FITC-anti human CD25 antibody (#356106, Biolegend), APC anti-human CD279 antibody (#329908 ...
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bioRxiv - Bioengineering 2021Quote: ... PE-anti human CD69 antibody (#310906, Biolegend), FITC-anti human CD25 antibody (#356106 ...
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bioRxiv - Bioengineering 2021Quote: ... APC anti-human CD279 antibody (#329908, Biolegend), PE/Cyanine 7 anti-human CD4 antibody (#317414 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human LFA1 (CD11a) FITC (Biolegend 363404) and anti-human PSGL1 BV421 (BD # 563961 ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107), and mouse anti-human V500-CD45 (BD # 560779 ...