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Citations for BioLegend :
601 - 650 of 3042 citations for Recombinant Human CBLB Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... anti-human CD123 (clone 6H6, BioLegend), anti-human CLEC9A (clone 8F9 ...
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bioRxiv - Immunology 2023Quote: ... anti-human CLEC9A (clone 8F9, BioLegend), anti-human CADM1 (clone 30 ...
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bioRxiv - Immunology 2023Quote: ... anti-human CD45RA (clone HI100, BioLegend), anti-human CD1C (clone L161 ...
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bioRxiv - Biochemistry 2024Quote: ... and anti-human CD3 (BioLegend, # 300420). Samples were acquired and recorded on a BD FACSAria II and data were analyzed with FlowJo™ software.
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bioRxiv - Microbiology 2023Quote: ... TotalSeq-B human CITEseq antibodies (BioLegend) used to subtype immune cells in the downstream data were ...
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bioRxiv - Cancer Biology 2020Quote: ... We next incubated them with 3.5 µg/ml biotinylated CD81 or CD63 antibody (BioLegend, clone 5A6, and H5C6) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Additionally, biotinylated antibodies were used for the staining ST2 (IL-33R) (Mdb, clone DJ8) and TSLPR (Biolegend, 22H9). For intracellular staining ...
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bioRxiv - Immunology 2020Quote: Saporin-linked ADCs targeting murine CD45.2 (CD45-SAP) and cKit (cKit-SAP) were generated by incubating biotinylated anti-mouse CD45.2 (clone 104, BioLegend) or biotinylated anti-mouse cKit (clone 2B8 ...
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bioRxiv - Immunology 2019Quote: ... Primary staining was done with an antibody against MR1 (Clone 26.5, gift from Ted Hansen, biotinylated by Biolegend) at 1:100 for 40 min on ice in the presence of 2% human serum ...
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bioRxiv - Immunology 2021Quote: ... and CD3+ cells were depleted from the donor cells using a biotinylated anti-mouse CD3e mAb (BioLegend; 100304) and the Miltenyi MACS® MicroBead (Miltenyi ...
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bioRxiv - Immunology 2020Quote: ... the sections stained for CD301b were incubated with 2.5 μg/ml biotinylated CD45R/B220 (clone RA3-6B2, BioLegend), followed by detection with SA-HRP and Alexa Fluor 647 Tyramide ...
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bioRxiv - Immunology 2021Quote: Biotinylated DPB1*04:01-monomers loaded with TFEYVSQPFLMDLE peptide (S167-180) were tetramerised using PE-Streptavidin (Biolegend, 405204). 1.1 volume PE-conjugated streptavidin was added to 1 volume of DPB1*04:01-monomer (1 mg/mL) ...
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bioRxiv - Immunology 2023Quote: ... Cells were next resuspended in 50 µl (per mouse) lavage buffer containing 0.5 µg biotinylated anti-mouse FcεRIα (clone MAR-1; Biolegend) and incubated 20 minutes at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and detection antibodies (biotinylated mouse anti-IgG1, anti-IgG2a or anti-IgE from BioLegend) were added and incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: Sorted mMDSCs (50,000) were stimulated with 50 ng/ml recombinant mouse M-CSF (Biolegend) in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Pathology 2021Quote: ... mice were injected with 5 μg recombinant mouse IL-10 (Biolegend, San Diego, CA) or sterile saline 24 hours after APAP challenge ...
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bioRxiv - Immunology 2022Quote: Mouse dorsal skin cells were stimulated with 40 ng/ml recombinant IL-23 (Biolegend) for 20 hr ...
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bioRxiv - Bioengineering 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend, 576406). Successful differentiation was confirmed by flow cytometry staining with antibodies against macrophage markers ...
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bioRxiv - Biochemistry 2022Quote: ... and cells were cultured overnight with 0.5 μg/mL recombinant IL-2 (Biolegend 589106) in RPMI + 10% heat-inactivated FBS until use.
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bioRxiv - Cancer Biology 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
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bioRxiv - Immunology 2023Quote: MutuDC1 cells were treated with mouse recombinant 20ng/ml IFN-β1 (Biolegend Cat.581302), mouse 80ng/ml IFN-α (Miltenyi Cat ...
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bioRxiv - Immunology 2023Quote: ... in the presence or absence of 20ng/ml of recombinant mouse IL-6 (Biolegend) and/or 200ng/ml of nivalenol (Cayman Chemical ...
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bioRxiv - Immunology 2023Quote: ... Recombinant Mouse GM-CSF (carrier-free; 20 ng/ml; BioLegend, San Diego, California, USA), 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Systems Biology 2023Quote: ... followed by stimulation with recombinant mouse IFNα or IFNβ1 (BioLegend Uk Limited, London, UK) at each condition.
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bioRxiv - Immunology 2024Quote: ... under Th1-polarizing condition (10 ng/mL recombinant mouse IL-12 (p70) (#577004, BioLegend), and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11 ...
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bioRxiv - Cell Biology 2023Quote: MAD-MB-453 cells were treated with recombinant hTRAIL (30 ng/ml, B294007, Biolegend) for 6 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and a further 1 h incubation with anti-His HRP conjugate (652504; BioLegend, San Diego, CA, USA) diluted (1:20000 ...
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bioRxiv - Microbiology 2021Quote: Mouse anti-HA.11 (Clone 16B12) and Mouse anti-HIS tag (Clone J099B12) were purchased from BioLegend. Rat anti-HA (Clone 3F10 ...
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bioRxiv - Bioengineering 2023Quote: The antibodies used for flow cytometry were as follows: anti-His Tag APC (BioLegend, USA, Cat: #362605), anti-human IgG (G18-145 ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and IFN-γ from human PBMCs were measured by LEGENDplex™ Human Inflammation Panel (Biolegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Human CD14+ cells were isolated from healthy donor PBMCs using MojoSort Human CD14 Selection Kit (Biolegend #480026), and were cultured with 50 ng/ml recombinant human GM-CSF (BioLegend #572904 ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with anti-human CD45 (PE) and anti-human CD117 (APC) (Biolegend, San Diego, CA, USA). CD45-low/CD117+ leukemia cells were FACS sorted (MA900 sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cells were sorted after staining with anti-human specific antibodies directed against EpCAM and CD298 (Biolegend) with simultaneous depletion of mouse cells stained with anti-mouse-specific antibodies directed against CD45 and CD31 (Biolegend).
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bioRxiv - Immunology 2020Quote: ... anti-human BDCA-2-PE (clone AC144; Miltenyi Biotech) and anti-human CD123-APC (clone 6H6; Biolegend). Cells were incubated with surface antibodies at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: Isolated CD4+T cells from healthy donors were incubated with sE2 or sE2F442NYT in the presence of 5µg/ml human anti-CD3 antibody and 1µg/ml human anti-CD28 antibody (BioLegend) for 2 hrs ...
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bioRxiv - Immunology 2023Quote: ... specific antibodies included anti-mouse CD3ε (145-2C11) and CD28 (37.51) or anti-human CD3 antibody (OKT3) and anti-human CD28 (CD28.2) (antibodies from Biolegend). For flow cytometry experiments ...
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2019Quote: ... OVA-specific IgG1 was detected using biotinylated anti-mouse IgG1 monoclonal antibody (0.02µg/mL RMG1-1, Biolegend – CA, USA) followed by SA-HRP (1:2000 dilution #P030701-2 ...
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bioRxiv - Immunology 2021Quote: ... PD-1 tetramers were prepared by incubating a 5:1 ratio of biotinylated PD-1 and PE streptavidin (BioLegend) for 10 minutes on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens: cKit (2B8, Biolegend), Sca1 (D7 ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend).
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bioRxiv - Immunology 2022Quote: ... enriched single-cell suspensions from tumor lysates were labelled with biotinylated anti-CD11b antibody (BioLegend, San Diego, CA, USA) and separated on MACS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and biotinylated isotype controls (Rat IgG2a,κ clone RTK2758 and Rat IgG2b,κ clone RTK4530) were purchased from Biolegend. Biotinylated antiPD-1 antibody (clone J43 ...
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bioRxiv - Immunology 2023Quote: ... fibroblasts and in vitro differentiated plasmablasts (generally <10% frequency) were removed using biotinylated anti-H-2Kd (Biolegend, cat: 116604) and anti-CD138 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... IFNγ ELISAs used the MD-1 clone for coating and a biotinylated 4S.B3 clone for detection prior to visualization with a streptavidin-HRP antibody (BioLegend).
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bioRxiv - Immunology 2024Quote: ... Truncated EGFR expression was detected using biotinylated cetuximab (Bristol-Myers Squibb) and a phycoerythrin (PE)-conjugated streptavidin antibody (BioLegend). T cell activation was assessed by fluorophore-conjugated monoclonal antibodies detecting CD137 (also known as TNFRSF9 ...