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Citations for BioLegend :
301 - 350 of 501 citations for Recombinant Bovine ICAM1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... Cells were then washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES) and stained with streptavidin-PerCP/Cy5.5 (BioLegend).
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bioRxiv - Immunology 2020Quote: ... The cells were stained in FACS buffer (PBS containing 0.5% bovine serum albumin and 0.05% sodium azide) with fluorescently labelled antibodies from BioLegend (CD11c-APC/Cy7 ...
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bioRxiv - Immunology 2022Quote: ... and resulting cells were cultured in complete RPMI-1640 medium containing various concentrations of recombinant murine GM-CSF (rGM-CSF, BioLegend, San Diego, CA, USA), GPI-GM-CSF ...
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bioRxiv - Immunology 2019Quote: Mice were intranasally dosed with 3 µg in 20 µl per mouse recombinant murine TNF-α (r-TNF-α, carrier-free, 575204, BioLegend Ltd, UK) every day for 3 days ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were incubated in high glucose Dulbecco’s’ modified Eagle medium (DMEM) (Capricorn) supplemented with 10% fetal bovine serum (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney epithelial) cells consisted of high glucose Dulbecco’s modified Eagle medium (DMEM) (Capricorn) with 10 % fetal bovine serum (FBS) (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Immunology 2023Quote: ... sections were blocked with PBS containing 1 % bovine serum albumin (BSA) and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
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bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
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bioRxiv - Immunology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend). SIINFEKL epitope-specific T cells were studied using APC-labelled H-2Kb/SIINFEKL pentamer (F093-84B-E ...
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bioRxiv - Cancer Biology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend) and PE-Cyanine 7 anti-mouse CD4 (25-0041-82 eBioscience) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cultures collected at different time points were subjected to TCA protein extraction (see Protein extraction by TCA) and immunoblotted with anti-Rpb1 (BioLegend 664912 clone 8WG16) or anti-Pgk1 (Abcam ab113687 clone 22C5D8 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then blocked (PBS-0.25% Triton X-100+ 1% BSA (bovine serum albumin)) for 1h at RT and then primary antibodies (anti-βIII tubulin (Tuj1, 1:1000, BioLegend), anti-NFH (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1-2 million HL-60 and THP-1 cells were resuspended in PBS containing 1% fetal bovine serum and stained with PE anti-human CD11b antibody (Biolegend). Stained cells were analyzed on FACScalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). For antigen-specific study ...
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bioRxiv - Immunology 2022Quote: ... Peridinin chlorophyll protein complex (PerCP) Cy5.5-conjugated: CD4 (Biolegend, 344608), CD26 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations were measured using ELISA MAX Deluxe Sets (Biolegend; IL1B cat ...
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bioRxiv - Immunology 2023Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). Cells were blocked with anti-CD16/CD32 antibody (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Systems Biology 2019Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) and incubated overnight with primary anti-XBP1s antibody (Biolegend Poly6195) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection using anti-HA (Covance 16B12, BioLegend, Dedham, MA, USA) or antiactin antibodies (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... expanded and screened for IGF2R protein by anti-IGF2R antibody (Biolegend) by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: Protein expression measurements were performed using TotalSeq-A reagents from Biolegend according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
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bioRxiv - Immunology 2020Quote: ... Bound proteins were detected by staining with fluorescently conjugated antibodies (BioLegend) and measured by flow cytometry ...
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bioRxiv - Biochemistry 2021Quote: ... USA)-conjugated anti-mouse CD206 and activated protein C (APC, BioLegend, USA)-conjugated anti-mouse CD86 at room temperature for 1 h without light ...
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bioRxiv - Genomics 2022Quote: Antibody-derived DNA tags for membrane proteins were purchased from Biolegend and listed in Table S5 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on blots were detected using anti-RpoB (Biolegend; 663905; AB_2566583), anti-RecA (Pocono Rabbit Farm & Laboratory ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... p38 mitogen-activated protein kinase (p38MAPK, mouse, 1:2000, Biolegend 602651), and phosphorylated-p38MAPK (phospho-p38MAPK ...
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bioRxiv - Immunology 2024Quote: ... peridinin chlorophyll protein-cyanin 5.5 (PerCP-Cy5.5)-anti-CD16 (3G8; BioLegend), Pacific Blue-anti-CD45 (HI30 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Secreted proteins were measured using the ELISA MAX Deluxe kits (Biolegend). Absorbance was measured at 450 nm and 570 nm using a Synergy H1 plate reader (BioTek) ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2021Quote: Isolated cells were stained at 4°C in FACS buffer (PBS supplemented with 2.5% bovine serum albumin) with and hematopoietic lineage markers including Ly6G (BioLegend, clone 1A8, 1:600), CD11b (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...