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Citations for BioLegend :
201 - 250 of 10000+ citations for Rabbit Anti Human IgG Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... non-specific binding was blocked using Fc Receptor blocking solution (Human TruStain FcX, BioLegend) and cells were stained with antibodies in PBS supplemented with 2% hiFCS for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: After blockade of Fc-receptors using Human TruStain FcX™ (BioLegend, London, United Kingdom), 5*106 PBMCs were stained with fluorescently labelled antibodies in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... re-suspended and labelled with Fc block (Human TruStain FcX™; Biolegend, London, UK) for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked by adding 2.5 μL Human TruStain™ FcX (BioLegend, #101320) per 50 μL of 200 000 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 5 µl of the Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend, #422302) were added to the sample and incubated for 10 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Non-specific bindings were blocked using Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend) for 10 min on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were stained for 30 minutes with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Immunology 2023Quote: ... 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Immunology 2024Quote: ... 100 µL 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... and rabbit polyclonal IgG control (Biolegend, CTL-4112).
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bioRxiv - Immunology 2021Quote: ... and then treated with Human TruStain FcX (Fc receptor blocking solution, Cat. No. 422302, BioLegend) for 5 mins ...
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bioRxiv - Genomics 2021Quote: ... and incubated with Human TruStain FcX™ (Fc Receptor Blocking Solution) (Biolegend, Cat#422302, USA) for 15 minutes on ice ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of Human TruStain FcX™ Fc Receptor Blocking Solution (1:400, BioLegend), and fixed with 0.5 mL methanol-free paraformaldehyde 4% (Thermo Scientific™ Pierce™ ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse Fc blocker (anti-mouse CD16/32, BioLegend 101320). Cells were washed twice with FACS buffer and strained through a 100 μm filter before sorting CD11b+ microglia and ACSA-2+ astrocytes on a FACS Aria III (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... After Fc blockade (anti-mouse CD16/32 TruStain, BioLegend), cells were stained using a standard flow cytometric staining protocol with fluorescently conjugated antibodies specific to CD3ε ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with anti-Fc receptor antibody (anti-CD16/32, Biolegend) and then stained with fluorochrome-conjugated antibodies in FACS buffer (PBS + 1% FBS + 2mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... either 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P ...
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bioRxiv - Immunology 2023Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
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bioRxiv - Cancer Biology 2021Quote: ... These steps were repeated with the secondary antibody as needed (IgG anti-rabbit PE, BioLegend, clone poly4064). After the last wash with FCB ...
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bioRxiv - Microbiology 2024Quote: ... and neurons were labelled with rabbit anti-tubulin beta 3 (TuJ-1) IgG at 1:500 (Biolegend), in PBS ...
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bioRxiv - Microbiology 2023Quote: ... for one hour followed by secondary antibody (anti-rabbit IgG AF555, #406412, BioLegend, San Diego, CA, USA) for 30 min at room temperature in 1X PBS/5% BSA/0.05% saponin ...
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bioRxiv - Bioengineering 2021Quote: ... containing 1/100 diluted anti-human CD3 or anti-human TCRα/β (Biolegend), both targeting the TCR complex ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... and rabbit isotype control IgG (BioLegend, 910805; 1 μg). The CUT&RUN library was prepared using a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Identity and purity of eluted proteins was assessed by coomassie staining on 10 % SDS PAGE followed by Western blotting with mouse anti-human basigin IgG (Biolegend). Fractions were pooled and concentrated using Amicon centrifugal filter units 100 kDa (Merck ...
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bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Fc receptors were blocked by incubating the cells with 50 μl Human TruStain FcX (Biolegend, #422302) for 10 minutes at room temperature prior to antibody staining and sorting ...
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bioRxiv - Immunology 2022Quote: ... Whole blood cell staining was performed using an Fc receptor blocking solution (Human TruStain FcX; BioLegend), the viability dye eFluor780 (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Cells were pelleted again and resuspended in 100 ul of Fc-Block (Human TruStain FcX, Biolegend), and 10 ul aliquot was taken for counting using K2 Cellometer (Nexcelom ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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bioRxiv - Microbiology 2022Quote: ... blocked with Fc Block for 20 min at room temperature (Human TruStain FcX, Biolegend Cat# 422302), and simultaneously stained for surface antigenic Site Ø ...
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bioRxiv - Immunology 2023Quote: Zombie Aqua Fixable Viability Dye and Human TruStain FcX Fc Receptor Blocking Solution were from BioLegend. CF700-SE and CF750-SE were bought from Biotium ...
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bioRxiv - Cancer Biology 2023Quote: ... Two million viable cells were resuspended in 100µl FACS buffer with 1 µl of Fc Block (anti-mouse Fc block CD16/32, Cat# 101302, Biolegend) for 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
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bioRxiv - Cancer Biology 2020Quote: ... and blocked Fc receptors with anti-CD16/32 (101319, BioLegend) for 20 minutes on ice and then stained with appropriate antibodies for 1 hour on ice ...
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bioRxiv - Immunology 2022Quote: ... and Fc receptor blockade (anti-mouse CD16/32, Biolegend 101319) were followed by incubation with antibodies against CD45 (Biolegend 103154) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with anti-Fc receptor antibody (Biolegend, 101320) on ice for 15 min followed by staining with the following antibodies for 30 min ...
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bioRxiv - Immunology 2023Quote: ... samples were stained with anti-human HLA-ABC and anti-human CD3PE (BioLegend, 317308) at RT in the dark ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD57-PB (BioLegend, Cat#322316] ...
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bioRxiv - Neuroscience 2021Quote: ... anti-human Tau (BioLegend, #806501), anti-dynamin (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD20 (BV785, Biolegend), and anti-human HLA-DR (BV711 ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD3 (AF700, Biolegend), anti-human CD32 (PE-Cy7 ...
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bioRxiv - Immunology 2020Quote: BV421 anti-human IgK (Biolegend)
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bioRxiv - Immunology 2020Quote: ... anti-human CD45 (BioLegend, UCHL1), anti-human CD56 (BD Biosciences ...