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Citations for BioLegend :
251 - 300 of 366 citations for RAB39B Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... or SIV Gag or Env peptides (1 μg/mL) for 1 hour with CD107a PECy5 (eBioH4A3, BioLegend) in R10 media before adding 1 mg/mL of Brefeldin A (Sigma-Aldrich®) ...
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APOE ε4 gene dose effect on imaging and blood biomarkers of glial reactivity and β-amyloid pathologybioRxiv - Neuroscience 2022Quote: ... Aβ peptides were immunoprecipitated from 250 μl of sample using 4G8 and 6E10 anti-Aβ antibodies (BioLegend) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads and a KingFisher Flex instrument (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Neuroscience 2022Quote: ... Muscle sections were incubated overnight at 4 °C in blocking solution (2 % BSA, 10 % Normal Goat Serum, 0.1 % Triton and PBS) with chicken anti-NFM (#822701, BioLegend, USA ...
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bioRxiv - Immunology 2020Quote: ... Blocking of Fc receptors in T2 cells was performed prior to staining using the TruStain FcX reagent (BioLegend, #422301). Staining with peptide-MHC dextramers was performed for 10 min at room temperature (RT) ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... In all assays 1 x 106 cells were first incubated with Human TruStain FcX (Fc Receptor Blocking Solution, Biolegend) or anti-human CD32 (FcγRII Blocker ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Immunology 2020Quote: ... followed by 10-minute incubation on ice with blocking buffer containing 10% mouse serum with 0.25 μg/ml anti CD16/CD32 (BioLegend). Cells where incubated with indicated antibodies (Supplementary Table 5 ...
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bioRxiv - Immunology 2023Quote: ... BSA/0.01% (v/v) Tween-20 in cold RNase-free PBS + 10 μl Human TruStain FcX blocking solution (#422301, BioLegend)) and incubated for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... tissues were blocked for endogenous avidin and biotin using an avidin biotin blocking kit according to the manufacturer’s instructions (BioLegend). Sections were then incubated with biotin conjugated goat anti-chicken (Vector Labs ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was done overnight at 4οC with the following antibodies diluted in blocking buffer: PAX6 (1:300, 901301 Biolegend), P63 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... and single cell preparations were obtained by passing the cell mixture through a PluriStrainer (500 – 70µM cell strainers; 43-50500, PluriSelect) before blocking with Fc block for 10 min (422302, BioLegend). CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401 ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Cells in each well were centrifuged at 350 g for 5 minutes and resuspended in 100 μL of PBS premixed with 0.5 μL of Trustain FcX plus blocking solution (BioLegend) on ice for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: 30 µL peptide-exchanged monomer formed in the above steps was mixed with 3.3 µL PE streptavidin (BioLegend) on a new plate and incubated on ice in the dark for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Thrombin Receptor Activator Peptide-6 (TRAP-6; 30μM) or Adenosine Diphosphate (ADP; 30μM) along with CD42b-BV421 (1:60, Biolegend), PAC-1 FITC (1:12 ...
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bioRxiv - Immunology 2021Quote: Biotinylated DPB1*04:01-monomers loaded with TFEYVSQPFLMDLE peptide (S167-180) were tetramerised using PE-Streptavidin (Biolegend, 405204). 1.1 volume PE-conjugated streptavidin was added to 1 volume of DPB1*04:01-monomer (1 mg/mL) ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
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bioRxiv - Microbiology 2019Quote: ... Human B cells were incubated in human Fc receptor blocking antibody then stained with Zombie Aqua fixable viability dye (1:500 dilution) (BioLegend), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Primary antibody labeling was performed in blocking buffer overnight at 4°C in a humidified chamber using the following antibodies: anti-CD4 Alexa Fluor 647 (BioLegend), goat anti-IgD purified (Cedarlane Labs) ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked for 10 min on ice following addition of 10 μL Fc blocking solution (BioLegend, cat. no. 422301), 4 μL of a 1% dextran sulfate solution (Research Products International ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the in vivo samples to obtain absolute counts of events acquired on the cytometer as well as using human and mouse Fc blocking antibodies (Biolegend) to reduce non-specific antibody staining ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then kept in PBS solution containing Triton X-100 and blocking agent (Normal Serum Block, CAT# 927502, Biolegend) for 2 h after which they were incubated in the solution containing ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked for 1 hour at 37⁰C with 300µl of Duolink® blocking solution then stained overnight at 4⁰C with purified mouse anti-Phosphoserine (Biolegend) or purified mouse IgG1 isotype control (clone MG1-45 ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation for 1h at RT in a 1:1000 dilution in blocking buffer of primary Ab (purified anti-Filaggrin polyclonal Ab, clone Poly19058, Biolegend). After washing ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in FACS stain buffer and resuspended in diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... Samples were then split into 7 FACS tubes and incubated with 100uL of diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... After blocking of free capture sites on the biosensors with an isotype control (10 µg/mL, human IgG1κ, clone QA16A12, BioLegend) for 600 s ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: All flow cytometry was performed at Fred Hutchison Cancer Center utilizing a 28-color panel focused on T cell phenotyping (Supplementary Table 1) [33] CVC and PBMC were incubated with Fc-blocking reagent (BioLegend) and fixable UV Blue Live/Dead reagent (ThermoFisher ...