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Citations for BioLegend :
401 - 450 of 889 citations for Phospho S6K1 T421 S424 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako ...
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bioRxiv - Microbiology 2022Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (diluted 1:1000) (BioLegend, item no. 901515) or rabbit polyclonal antibody (pAb ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were stimulated with 2 μg/mL of indicated COVID-19 antigen peptide pools (RBD or Spike or NP) in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37 □ overnight ...
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bioRxiv - Immunology 2022Quote: ... MR1 surface expression was evaluated by flow cytometry with APC-labeled mouse anti-MR1 mAbs 26.5 (Biolegend, Cat#361108) and APC-labeled mouse IgG2a (clone MOPC-173 Biolegend ...
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bioRxiv - Immunology 2022Quote: ... CD69-) from OT-I mice were stimulated with plate-bound anti-CD3 mAb (5µg/ml, clone 145-2C11, Biolegend), and soluble anti-CD28 (1µg/ml ...
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bioRxiv - Immunology 2022Quote: ... female C57BL/6 mice were treated with 20 μg of α-CCR2 mAb (clone: MC21, RRID:AB_2314128; (23) or isotype control antibody (Rat IgG2b, Biolegend) in 200 μl of PBS via daily intra-peritoneal (i.p. ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Immunology 2022Quote: ... Cell cultures were stimulated for 3 days with either 100 ng/mL soluble or 1.25 μg/mL immobilised (plate-bound) anti-CD3 mAb (clone OKT3, BioLegend) in combination with 1 μg/mL soluble anti-CD28 mAb (clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: Plates (48- or 96-well, flat bottom) were coated with 10 μg/ml αCD3ε mAb (BioLegend, San Diego, CA) and incubated at 37°C for 5 hours ...
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bioRxiv - Immunology 2023Quote: ... Cell mixtures were then washed in PBS and stained with mAbs to CD56 (FITC or BV605, Biolegend, #318304, #318334), NKG2A (PE ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed with the anti-Aβ mAb 4G8 conjugated with Alexa Fluor 647 (BioLegend) and R3VQ conjugated with 5-TAMRA (see Sup ...
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bioRxiv - Immunology 2023Quote: ... aureus Newman (from 2.106 to 2.104/well) in the presence of anti-human CD107a-AF647 mAb (clone H4B4, Biolegend, France) added at the beginning of stimulation in serum and antibiotic free RPMI media in 96 well round bottom plates ...
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bioRxiv - Immunology 2024Quote: ... TCRs were transiently transfected into 293T-mCD3-GFP cells and stained separately with PE anti-mTCRβ mAb (Biolegend, 109208) and HLA-A3 tetramers refolded with WT– and G12V-KRAS 10mer peptides ...
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bioRxiv - Immunology 2021Quote: Sorted mMDSCs (50,000) were stimulated with 50 ng/ml recombinant mouse M-CSF (Biolegend) in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
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bioRxiv - Pathology 2021Quote: ... mice were injected with 5 μg recombinant mouse IL-10 (Biolegend, San Diego, CA) or sterile saline 24 hours after APAP challenge ...
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bioRxiv - Immunology 2022Quote: Mouse dorsal skin cells were stimulated with 40 ng/ml recombinant IL-23 (Biolegend) for 20 hr ...
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bioRxiv - Bioengineering 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend, 576406). Successful differentiation was confirmed by flow cytometry staining with antibodies against macrophage markers ...
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bioRxiv - Cancer Biology 2022Quote: ... The “rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). After 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and cells were cultured overnight with 0.5 μg/mL recombinant IL-2 (Biolegend 589106) in RPMI + 10% heat-inactivated FBS until use.
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bioRxiv - Cancer Biology 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: MutuDC1 cells were treated with mouse recombinant 20ng/ml IFN-β1 (Biolegend Cat.581302), mouse 80ng/ml IFN-α (Miltenyi Cat ...
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bioRxiv - Immunology 2023Quote: ... in the presence or absence of 20ng/ml of recombinant mouse IL-6 (Biolegend) and/or 200ng/ml of nivalenol (Cayman Chemical ...
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bioRxiv - Immunology 2023Quote: ... Recombinant Mouse GM-CSF (carrier-free; 20 ng/ml; BioLegend, San Diego, California, USA), 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Systems Biology 2023Quote: ... followed by stimulation with recombinant mouse IFNα or IFNβ1 (BioLegend Uk Limited, London, UK) at each condition.
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bioRxiv - Immunology 2024Quote: ... under Th1-polarizing condition (10 ng/mL recombinant mouse IL-12 (p70) (#577004, BioLegend), and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11 ...
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bioRxiv - Cell Biology 2023Quote: MAD-MB-453 cells were treated with recombinant hTRAIL (30 ng/ml, B294007, Biolegend) for 6 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with different combinations of 400 ng/mL human recombinant sFasL (Biolegend), 40 μM Fasudil HCl (SellekChem) ...
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bioRxiv - Immunology 2021Quote: ... or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus, referred to as anti-h/pCD47; Biolegend). The level of cell surface CD47 is expressed as median fluorescent intensity (MFI) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel Parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2022Quote: ... T cells were washed in PBS/1% FCS and stained using the following mAbs in appropriate combinations: anti-OX40-PE-Cy7 (BioLegend), anti-GITR- BV421 (BioLegend) ...
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bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
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bioRxiv - Immunology 2022Quote: ... Anti-Mac-1 was reconstituted by mixing anti-CD11b (clone ICRF44) and CD18 (clone TS1/18) mAbs at a final concentration of 10 µg/mL for each (BioLegend). Anti-CD3 mAbs (clone UCHT1 ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Immunology 2019Quote: Macrophages were stained for 40 min at 4°C with fluorophore-conjugated mAb FITC-anti-CD36 (clone 5-271, Biolegend), or PE-anti-CD209 (clone 120507 ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with K562 at a 1:1 E/T ratio with addition of a CD107a FITC mAb (H4A3, BioLegend) in a 96-round bottom plate with a centrifugation step of 5min at 500rpm ...
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2019Quote: ... and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb (all were obtained from BD Biosciences and Biolegend). Data were acquired on a BD LSR Fortessa flow cytometer with BD FACS Diva software and analyzed using FlowJo Software version 9.5 (Tree Star ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Immunology 2020Quote: ... The following surface antigens were detected by the indicated mAb clone: B220 (RA3-6B2-BV785 and RA3-6B2-BV421, BioLegend), CD21 (7EG-PerCP-Cy5.5 ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...