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Citations for BioLegend :
451 - 500 of 9382 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with Live/Dead Yellow (1:1000) followed by Fc block (1:200; BioLegend, San Diego, CA), and stained with antibody cocktails for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Roughly 0.5 x 106 cells were washed in FACS buffer (PBS + 3% FCS + 0.1% sodium azide) and stained with either FITC-coupled anti-FcεR1 (1:100, clone MAR-1, BioLegend, #134306) and PE-coupled anti-CD117 (1:100 ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with an Alexa Fluor 647-labeled mouse monoclonal antibody against the human IgG1-Fc (Biolegend, San Diego, USA, cat #409320) and analyzed in a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Immunology 2020Quote: Fc block was performed using TruStain FcX™ CD16/32 antibody (anti-mouse) to avoid non-specific binding (101320 Biolegend, UK) according to the supplier protocols The fluorochrome-conjugated antibodies used in the panel were obtained from Biolegend ...
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bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... TCRβ (H57-597, BV605), NK-1.1 (PK136, BV650), and PD-1 (RPM1-30, PE/Dazzle 594) were purchased from BioLegend (San Diego, CA). Antibodies against CD4 (GK1.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with biotin-anti-human CD274/PD-L1 (BioLegend) on ice for 20 min ...
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bioRxiv - Immunology 2023Quote: ... anti-CD274 (B7-H1, PD-L1)-PE/Dazzle (BioLegend, clone, 10F.9G2, #124323), anti-TER-119-FITC (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in FACS buffer with FC block (anti-CD16/CD32, 1:500, Biolegend 101330) and incubated for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Immunology 2024Quote: ... human cells were blocked with Human TruStain FcX Fc Receptor Blocking Solution (Biolegend, #422302, 1:100) in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... the pellets were resuspended in 90 µL FACS buffer with Fc block (4G2, 1:90, BioLegend) and transferred to a 96 well-plate ...
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... before Fc blockade with TruStain FcX (BioLegend) and subsequent surface marker staining ...
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bioRxiv - Cell Biology 2020Quote: ... Isotype Ctrl (clone Poly29108, Biolegend 910801, FC). For Odyssey detection of western blots ...
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bioRxiv - Immunology 2020Quote: ... Fc receptor blocking antibody (CD16/CD32; Biolegend), anti-EpCAM (clone G8.8 ...
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bioRxiv - Immunology 2022Quote: ... Fc receptor-blocking antibody (CD16/CD32; BioLegend), anti-EpCAM (clone G8.8 ...
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bioRxiv - Cancer Biology 2021Quote: ... or mPD-L1-FC (Biolegend, Cat#758208) and incubated at 4°C overnight before seeding cells.
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bioRxiv - Microbiology 2021Quote: ... in the presence of Fc Block (BioLegend) and fixable viability dye (Ghost Violet 510 ...
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bioRxiv - Immunology 2022Quote: ... APC-conjugated anti-human IgG (Fc) (Biolegend), APC-Cy7-conjugated anti-CD3 antibody (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... and Fc-Block (BD Biosciences or Biolegend) for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... with anti-CD16/32 Fc-Block (BioLegend), then stained with the following antibodies for 30 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and Fc Block (CD16/CD32, Tonbo, BioLegend) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked (αCD16/32, BioLegend) followed by surface staining and analyzed by flow cytometry on an LSRFortessa or FACSymphony (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Fc-blocked for 10 minutes (FcX, Biolegend) and stained with the surface antibody cocktail (reconstituted in CSM ...
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bioRxiv - Immunology 2022Quote: ... added 1uL of TruStain FC Blocker (Biolegend) per 1e6 cells ...
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bioRxiv - Genomics 2022Quote: ... Fc block (BioLegend, USA; 5μl per sample); wash buffer (600μl per sample) ...
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bioRxiv - Immunology 2022Quote: After Fc-blocking using FcX reagents (BioLegend), cells were incubated with primary antibodies in FACS buffer for 30 min at 4°C and if necessary ...
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bioRxiv - Cell Biology 2023Quote: ... Human B7-H2-Fc Chimera (Biolegend, 599904), Human SNAP43 (SNAPC1 ...
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bioRxiv - Immunology 2023Quote: ... blocked with Fc receptor (Biolegend, CA, USA), and stained with CD3-PE (clone HIT3a ...
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bioRxiv - Immunology 2023Quote: ... The anti-human IgG Fc Antibody (BioLegend) and the Sheep Anti-Rat IgG Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... Human TruStain FcX FC blocking reagent (BioLegend) was added according to manufacturers’ instructions for 10min on ice ...
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bioRxiv - Immunology 2023Quote: ... Samples were Fc-blocked (BioLegend, cat #422302) on ice for 5 minutes and stained with anti-β2m Platinum (Pt ...
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bioRxiv - Immunology 2023Quote: ... Fc-Block CD16/32 (Cat# 156604-BioLegend) was used to restrict non-specific antibody binding ...
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bioRxiv - Immunology 2023Quote: ... and Fc block (Biolegend, Cat. no. 422302) for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in Fc-Block (BioLegend) containing PBS/2% FCS ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in Fc-Block (BioLegend) containing PBS/2% FCS buffer ...
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bioRxiv - Immunology 2024Quote: ... and then incubated with Fc block (Biolegend) in FACS buffer (PBS ...