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Citations for BioLegend :
451 - 500 of 984 citations for PB Cadherin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were incubated with the peptides for 1 hour following fixation with formaldehyde and staining with cytochrome c antibody (BioLegend, Alexa Fluor® 647 anti-Cytochrome c - 6H2.B4 ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... biotinylated NP peptide loaded SLA monomers were freshly assembled into tetramer with streptavidin BV421 or BV650 (both from Biolegend, UK). Two million mononuclear cells were incubated with protease kinase inhibitor in PBS for 30 minutes at 37°C and tetramers added to the cells on ice for another 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured in stimulation media without peptide stimulation or with phorbol 12-myristate 13-acetate (PMA) and ionomycin (BioLegend), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... PB was depleted of red blood cells (RBC Lysis Buffer; Biolegend) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 3% BSA/PBS cell staining buffer (BioLegend) and incubated at 37°C for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... and FAS-AF488 (for FAS analysis; eBioscience) or TCRβ-PB (Biolegend), CD8-PE ...
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bioRxiv - Immunology 2019Quote: ... Cells were then washed with PBS and then permeabilization buffer (BioLegend) and stained in permeabilization buffer with antibodies targeting MAIT cell TCR components (Vα7.2 TCR ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were resuspended in PBS with Zombie Violet viability dye (Biolegend) and incubated at 4°C for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... 100ul of PBS supplemented with LEAF purified anti-CD3 (OKT3, Biolegend) at 0.25ug/ml was added to each well and incubated at least 12 hours at 4°C ...
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bioRxiv - Immunology 2023Quote: ... washed with PBS and stained with Zombie Violet fluorescent dye (Biolegend) per manufacturer’s instructions for live/dead discrimination ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with PBS and permeabilized with perm buffer (Biolegend), and labelled with anti-human active caspase-3 antibody (Asp175 ...
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bioRxiv - Immunology 2023Quote: ... washed in PBS and stained with fixable viability Zombie dyes (Biolegend) diluted in PBS ...
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bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then incubated overnight at 4°C with primary antibody diluted in blocking solution (rabbit anti-FLAG, Sigma, 1:250 or mouse anti-Tuj1, Biolegend 1:500). After washing in PBS and incubation with secondary antibody (Alexa 647 anti-goat IgG 1:500 ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Stain 510 for 15 minutes at 4 °C followed by anti-Fc blocking reagent (Biolegend, Cat# 101320) for 10 minutes prior to surface staining ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained using a three-colour protocol with 1:100 of each of the following antibodies in IF blocking buffer at 4 °C O/N: Alexa Fluor 488® anti-mouse GL-7 (GL-7; Biolegend, #144612), PE anti-mouse CD3 (145-2C11 ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Pathology 2024Quote: ... The membrane was blocked in Licor Intercept Blocking Buffer for 1hour at room temperature then incubated with Cd9 antibody (HI9a Biolegend Cat 312112) at 1:5000 overnight at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were live-stained for two hours using a conjugated antibody targeted against an OVA257-264 peptide antigen (BioLegend, 141605). The cells were then fixed with 4% paraformaldehyde and imaged using the PerkinElmer Operetta CLS High-Content Analysis System ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... The levels of peptide-loaded MHC-I on the beads were tested using the PE-conjugated W6/32 antibody (Biolegend, 311406). Binding levels were measured in Luminex 100 Liquid Analyzer System and the results analyzed in GraphPad Prism v9.
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Immunology 2019Quote: ... After isolation cells were treated with Fc receptor blocking solution and stained with anti-ICAM-1-PE (1 μg/ml, HA58; Biolegend, San Diego, USA), anti-VCAM-1-BV711 (0.5 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Genomics 2021Quote: ... Sections were incubated with the following primary antibodies at the indicated dilution in blocking buffer overnight at 4°C: NeuN (BioLegend 1B7, cat #834502), p65 (BioLegend Poly6226 ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained in PBS with TruStain fcX (BioLegend, Cat. No. 101320) to reduce non-specific antibody binding ...
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bioRxiv - Cell Biology 2020Quote: ... Single cells were stained with Zombie Aqua (Biolegend, 1:1000 in PBS) for 15min at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in PBS and Alexa Fluo 488 Zombie Green (BioLegend) was added at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... counted and labelled with carboxyfluorescein succinimidyl ester (CFSE) (Biolegend; 5μM in PBS). The CFSE-labelled J774A.1 cells were collected by centrifugation (1500rpm ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with a cocktail of antibodies against TCRβ-PB (Biolegend), CD8-FITC ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with a cocktail of antibodies against TCRβ-PB (Biolegend), CD8-FITC ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in PBS before staining with anti-HSA-APC (Biolegend) at 1:200 dilution for 30mins at 4C ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed with PBS and incubated with Zombie Violet (BioLegend #423114) for 30 minutes in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... pelleted and resuspended in Zombie NIR dye in PBS (1:7000; Biolegend). After 30 minutes rocking at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with CFSE (1:6000 in PBS, Biolegend, 423801) for 20 min at 37°C and washed with 2% BSA in FBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in 1:1 PBS-diluted Fixation Buffer (Biolegend), comprising ~2% PFA ...
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bioRxiv - Cell Biology 2023Quote: ... Cell were washed in PBS and fixed using commercial fixation buffer (Biolegend). After washes ...
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bioRxiv - Immunology 2023Quote: Single cells were stained with Zombie Aqua (Biolegend, 1:1,000 in PBS) for 15 min at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with Zombie NIR (BioLegend, 1:1000 diluted in PBS) for 20 min at 37°C (to discriminate live and dead cells in the later analysis) ...
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bioRxiv - Immunology 2024Quote: Cells were stained in PBS with TruStain fcX (BioLegend, Cat. No. 101320) to reduce non-specific antibody binding ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).