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Citations for BioLegend :
101 - 150 of 302 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... or DDX39B-depleted (Sh3 and Sh5) MT-2 cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set (BioLegend). The permeabilized cells were stained with antibodies against FOXP3 conjugated with Alexa Fluor 647 (AB_2539534 ...
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bioRxiv - Immunology 2021Quote: ... washed twice with True Nuclear Permeabilization/Wash buffer and then stained for 1 hour at room temperature with FoxP3 BV421 (206D, BioLegend), Ki-67 BV711 (Ki-67 ...
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bioRxiv - Neuroscience 2022Quote: ... samples that passed visual inspection were subjected to flow cytometry with the fluorescent nuclear stain DRAQ7TM (Biolegend, San Diego, CA) and an Alexa FluorTM 488-conjugated antibody against the neuronal intermediate filament beta-tubulin III (TuJ1) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401, Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... intracellular staining was performed on single cell suspensions according to instructions provided by True-Nuclear™ Transcription Factor Buffer Set (Biolegend). The antibodies used were anti-IL-1β PE (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... The purity of the sorted Treg subsets was assessed by staining the cells with FoxP3-APC mAb using the FoxP3 Fix/Perm Buffer Set for nuclear staining (Biolegend, USA). Over 95% of purity was detected in all cases analyzed ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplexing was performed by washing and hashing nuclei from each sample with one of six unique TotalSeq™-A anti-nuclear pore complex antibodies (BioLegend). Two multiplexed sequencing batches were then sequenced by the Oxford Genomics Centre (Wellcome Centre for Human Genetics ...
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bioRxiv - Genomics 2022Quote: ... supernatants were removed and cells fixed according to the manufacturer’s instructions utilizing the True-Nuclear™ Transcription Factor Buffer Set (Biolegend, 424401). Intracellular staining was performed according to manufacturer’s instructions before FACS analysis was carried out ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed in FACS buffer three times and fixed overnight in 200 µL True Nuclear fixation buffer (Biolegend #73158, #71360) at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were then fixed and permeabilized for 30 minutes followed by intra-nuclear staining for FOXP3 for an additional 4hours using FOXP3 Fix/Perm Buffer set (Biolegend, San Diego, CA).
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bioRxiv - Cell Biology 2022Quote: ... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
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bioRxiv - Immunology 2023Quote: Biotinylated S1 protein was obtained commercially (BioLegend). A 4X molar excess was incubated with streptavidin-Alexa Fluor 647 (0.5 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Immunology 2021Quote: ... Protein tyrosine phosphorylation was detected by PY20 (Biolegend) or 4G10 (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... myelin basic protein (mouse monoclonal, BioLegend, Dedham, MA), PDGFRα (goat polyclonal ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
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bioRxiv - Immunology 2023Quote: ... RBD protein conjugated with PE-streptavidin (BioLegend, 405204) and APC-streptavidin (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
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bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
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bioRxiv - Immunology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend). SIINFEKL epitope-specific T cells were studied using APC-labelled H-2Kb/SIINFEKL pentamer (F093-84B-E ...
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bioRxiv - Cancer Biology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend) and PE-Cyanine 7 anti-mouse CD4 (25-0041-82 eBioscience) ...
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bioRxiv - Bioengineering 2024Quote: ... Magnetic beads displaying recombinant IL-6 protein (BioLegend #570806) were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203) ...
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bioRxiv - Immunology 2023Quote: ... All recombinant proteins and compounds were purchased from Biolegend, USA ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). For antigen-specific study ...
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bioRxiv - Immunology 2022Quote: ... recombinant Siglec 5-Fc chimeric protein (Biolegend, San Diego, CA) was added and incubated for 2hrs at RT ...
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bioRxiv - Immunology 2022Quote: ... Peridinin chlorophyll protein complex (PerCP) Cy5.5-conjugated: CD4 (Biolegend, 344608), CD26 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations were measured using ELISA MAX Deluxe Sets (Biolegend; IL1B cat ...
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bioRxiv - Immunology 2023Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). Cells were blocked with anti-CD16/CD32 antibody (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection using anti-HA (Covance 16B12, BioLegend, Dedham, MA, USA) or antiactin antibodies (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... expanded and screened for IGF2R protein by anti-IGF2R antibody (Biolegend) by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: Protein expression measurements were performed using TotalSeq-A reagents from Biolegend according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
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bioRxiv - Immunology 2020Quote: ... Bound proteins were detected by staining with fluorescently conjugated antibodies (BioLegend) and measured by flow cytometry ...
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bioRxiv - Biochemistry 2021Quote: ... USA)-conjugated anti-mouse CD206 and activated protein C (APC, BioLegend, USA)-conjugated anti-mouse CD86 at room temperature for 1 h without light ...
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bioRxiv - Genomics 2022Quote: Antibody-derived DNA tags for membrane proteins were purchased from Biolegend and listed in Table S5 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on blots were detected using anti-RpoB (Biolegend; 663905; AB_2566583), anti-RecA (Pocono Rabbit Farm & Laboratory ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... p38 mitogen-activated protein kinase (p38MAPK, mouse, 1:2000, Biolegend 602651), and phosphorylated-p38MAPK (phospho-p38MAPK ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
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bioRxiv - Synthetic Biology 2023Quote: ... Secreted proteins were measured using the ELISA MAX Deluxe kits (Biolegend). Absorbance was measured at 450 nm and 570 nm using a Synergy H1 plate reader (BioTek) ...
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bioRxiv - Immunology 2024Quote: ... peridinin chlorophyll protein-cyanin 5.5 (PerCP-Cy5.5)-anti-CD16 (3G8; BioLegend), Pacific Blue-anti-CD45 (HI30 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Biochemistry 2022Quote: ... or 0.5ng/mL of Wnt3a recombinant protein (BioLegend, San Diego, CA, #772301). Luciferase activity was measured by bioluminescence (Promega Dual-Glo Luciferase Assay System ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Genomics 2022Quote: ... Proteins of interest were visualized using an anti-Flag-PE (BioLegend, 637310), an anti-HA-Alexa647 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... WT biotinylated RBD protein conjugated with TotalSeq™-C0973 Streptavidin (BioLegend, 405275) and TotalSeq™-C0974 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...