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Citations for BioLegend :
401 - 450 of 3389 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-human CD16 (Biolegend), BUV395 anti-human CD19 and CD123 (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... BV605 anti-human CD45 (Biolegend), APC anti-human CD66b ...
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bioRxiv - Cancer Biology 2023Quote: ... or Human TruStain FcX (BioLegend). The following antibodies were used for FACS analysis ...
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bioRxiv - Immunology 2023Quote: ... anti-human SLAMF6 (BioLegend #317202) and anti-human SLAMF3 (BioLegend #326109).
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bioRxiv - Immunology 2023Quote: ... anti-human IL-6 (Biolegend), or isotype control antibodies for 30 min.
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bioRxiv - Cell Biology 2023Quote: ... Conjugated human EPCAM antibody (Biolegend) was added for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... anti-human CD19 (HIB19, BioLegend), anti-human CD56 (MEM-188 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human TrueStain FcX (Biolegend, 422302) or mouse FcR blocker (Miltenyi 130-092-575 ...
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bioRxiv - Immunology 2024Quote: ... recombinant human CD40L (TNFSF5; BioLegend) was added to the media ...
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bioRxiv - Biochemistry 2024Quote: ... anti-human CD19 (BioLegend, # 302212), anti-human CD33 (BioLegend ...
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bioRxiv - Biochemistry 2024Quote: ... anti-human CD33 (BioLegend, # 366608), anti-human CD34 (BioLegend ...
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bioRxiv - Biochemistry 2024Quote: ... anti-human CD235a (BioLegend, # 349104), anti-human CD19 (BioLegend ...
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bioRxiv - Biochemistry 2024Quote: ... anti-human CD34 (BioLegend, # 343504), and anti-human CD3 (BioLegend ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Neuroscience 2021Quote: ... Single Cell suspensions were incubated with an anti-FcγIII/II receptor antibody (clone 93, eBioscience) to block unspecific binding and Zombie NIR™ (Biolegend), a fixable viability dye ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat ...
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bioRxiv - Neuroscience 2021Quote: The myelin-depleted single-cell suspension obtained above was incubated (15 min on ice) with anti-mouse CD16/CD32 antibody to block Fc receptors and subsequently incubated with anti-CD11b PEcy7 (Biolegend), anti-CD45 APC (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... To prevent antibodies from binding to Fc-receptors we treated the cell suspension with Fc-block (Biolegend #101301; 1:50) prior using other antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with a staining mix containing Fc receptor blocker (TruStain fcX™, BioLegend, Amsterdam, The Netherlands, 1:100), viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Immunology 2024Quote: ... FcγII/III receptors were blocked by prior 10 min incubation with Fc block (anti mouse CD16/32 antibody Clone 93, Biolegend) on ice.
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Immunology 2021Quote: ... 5 ng/ml IL-5 (Biolegend; 581504), and 20 ng/ml IL-2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... streptavidin cyanine 5 (5 μg/mL, BioLegend) for 1 h ...
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bioRxiv - Microbiology 2021Quote: THP-1 human macrophages were treated with mouse anti-human Fc block (Ref: 422302, BioLegend), for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... which consisted of anti-human Alexa Fluor® 700 anti-human CD3 (BioLegend, cat. 300324), Brilliant Violet 785™ anti-human CD4 (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Th1 cell released cytokines were analyzed by LEGENDplexTM Human Th1 Panel (Biolegend, Cat# 741035). Five human T cell cytokines including IL-2 ...
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bioRxiv - Immunology 2021Quote: ... RBD was then detected using an Alexa Fluor 488 conjugated anti-His antibody (clone J099B1, Biolegend). Cells were analyzed on a flow cytometry (BD).
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bioRxiv - Biochemistry 2022Quote: ... one slide was probed with Alexa Fluor® 647 anti-his antibody (1:250, BioLegend, 362611). Sera/plasma samples were single or serial diluted (three-fold dilutions from 1:90 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 antihuman CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... cells were initially blocked with anti-mouse Fcγ III/II receptor (CD16/CD32) antibodies and dead cells excluded by staining with a fixable live/dead cell-exclusion dye (BioLegend, USA). Then ...
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bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... The Netherlands and anti-human CD14-FITC (RRID:AB_830677) and anti-human CD163-AF647 (RRID:AB_2563475) from BioLegend, San Diego ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12p70 and recombinant human IL-18 at 10ng/ml (Biolegend; San Diego, CA).
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bioRxiv - Microbiology 2022Quote: ... activated with 5µg/ml human anti-CD3 antibody and 1µg/ml human anti-CD28 antibody (BioLegend), and incubated in the presence or absence of 2.5µg/ml sE2 or sE2F442NQT for 3 days ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated with Human FcX (BioLegend) to prevent non-specific antibody binding ...
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bioRxiv - Systems Biology 2020Quote: ... a BV650-anti-Human-CD11b (BioLegend) and a PE-anti-Human-CD15 (MiltenyiBiotec ...