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Citations for BioLegend :
51 - 100 of 3147 citations for Nectin 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used in each sample were: mouse anti-HA 1:2000 (Biolegend, 901513; HEK293), rabbit anti Cav2.3 N-terminus 1:250 (Covalab ...
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bioRxiv - Immunology 2023Quote: Human: Anti-human CD141 (clone M80, BioLegend), anti-human CD45RA (clone HI100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified anti-His tag mouse antibody was from BioLegend (USA). Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... incubating the cells with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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bioRxiv - Immunology 2019Quote: ... GolgiPlug™ and GolgiStop™ were added 3 h before cells were processed for surface and intracellular staining for CD4 (BV785-conjugated anti-human; OKT-4, BioLegend), CD8 (APC-H7-conjugated anti-human ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed with FACS buffer and labeled with PE conjugated mouse anti-human TNFRSF12A antibody (clone ITEM-4, Biolegend) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Biochemistry 2024Quote: ... following an overnight incubation at 4°C with the primary antibody against residues 103-108 of human α-synuclein (BioLegend, 807801 [4B12] 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells stably expressing EPO-eGFP and A1At-mCherry were incubated with 1ug/mL Brefeldin A (Biolegend) for 12 hr ...
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend). Phenotyping was performed on a NovoCyte Advanteon flow cytometer and FACS was performed on a BD LSRII.
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bioRxiv - Immunology 2022Quote: ... using human (BioLegend) and murine (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... anti-human (Biolegend): GZMB-APC (Cat.372203) ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 647 anti-human human CD3 Antibody (BioLegend, 300416), Biotin anti-human CD3 Antibody (BioLegend ...
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bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
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bioRxiv - Pathology 2019Quote: ... The ears were fixed in 4% paraformaldehyde for 20 minutes at 4°C and incubated overnight in 0.05% Triton X-100 in PBS containing anti-human CD31 (Biolegend, San Diego, CA, USA) antibody conjugated to either PE or AF647 in a 1:200 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
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bioRxiv - Cancer Biology 2023Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
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bioRxiv - Immunology 2021Quote: ... human CD4-APC (Biolegend), human FoxP3-APC (eBioscience) ...
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bioRxiv - Neuroscience 2021Quote: ... Human TruStain FcXTM (Biolegend). Protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), human CD25 (BioLegend #302606) ...
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bioRxiv - Cancer Biology 2023Quote: ... human FOXP3 (BioLegend #320116), Alexa Fluor® 647-conjugated anti-mouse integrin αvβ5 Ab (clone ALULA ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), Zombie NIR™ Fixable Viability Kit (BioLegend #423106 ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD25 (BioLegend #302606), human FOXP3 (BioLegend #320116) ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD8 (BioLegend #344712), human CD45 (BioLegend #304014) ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD3 (BioLegend, #317324), mouse CD45 (BioLegend #103130) ...
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bioRxiv - Immunology 2024Quote: ... Human TruStain FcX (BioLegend) was added at 1:20 to block Fc receptors for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... human TGFβ1 (580706; BioLegend), human IL-12 (219-IL;R&D Sys-tems) ...