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Citations for BioLegend :
251 - 300 of 506 citations for Native Red Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... After a quick wash with DPBS cells were stained with Zombie Red™ Fixable Viability Kit (#423110, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... A far-red BST2 antibody was used to label BST2-expressing cells (diluted 1:40, BioLegend, cat. 348416). Cell viability was determined by Sytox Blue (diluted 1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... After fixation and permeabilization, intracellular cytokine staining was performed with fluorescent monoclonal antibody against IFNγ (Phycoerythrin, eBioscience) and TNFα (Allophycocyanin, BioLegend). Cells were analyzed by flow cytometry on a BD LSR II flow-cytometer (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Neuroscience 2022Quote: ... samples that passed visual inspection were subjected to flow cytometry with the fluorescent nuclear stain DRAQ7TM (Biolegend, San Diego, CA) and an Alexa FluorTM 488-conjugated antibody against the neuronal intermediate filament beta-tubulin III (TuJ1) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2022Quote: ... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... passed through a 100-µm cell strainer and red blood cells were lysed with the RBC lysis buffer (Biolegend). To differentiate between live and dead cells ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were spun down and resuspended in 5 mL of 1x Red Blood Cell Lysis buffer (Biolegend) and incubated on ice for 5 minutes with occasional swirl ...
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bioRxiv - Bioengineering 2023Quote: Lung single-cell suspensions were stained for 30 min at RT with Zombie Red™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Pathology 2023Quote: ... Single cell suspensions and removal of red blood cells (RBC) was performed with RBC lysis buffer diluted in water according to manufacturer’s recommendations (BioLegend). For cryopreservation ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellets/red blood cells (lower layer) were then resuspended with the RBC Lysis Buffer (Biolegend, cat.420301) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... congenic CD45.1 mouse spleen was isolated and red blood cells were lysed with RBC lysis buffer (BioLegend, CA, USA). Leukocytes were then resuspended in complete RPMI with 1µg/mL high molecular weight Poly(I:C) ...
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bioRxiv - Immunology 2019Quote: ... T cells were pelleted in a V-bottom plate and resuspended in 40 µl PBS with 2% BSA and fluorescent 9V pMHC tetramers that were produced with Streptavidin-PE (Biolegend, 405204) and used at a predetermined dilution (1:100-1:1000) ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Immunology 2021Quote: ... and a cocktail of fluorescent antibodies: 1 µL each of anti-human CD3 (Brilliant Violet 421-conjugated, Biolegend 344834, clone SK7), anti-human CD69 (PerCP-eFluor710-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... Later 25µl of the following mixture of fluorescent-labeled antibodies was added to the samples: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: Cytokines from undiluted samples from chips/plates were analyzed by fluorescent bead-based multiplex sandwich immunoassays (Legendplex™ Human CD8/NK Mix and Match Subpanel; BioLegend) containing capture beads targeting IL-2 ...
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bioRxiv - Immunology 2020Quote: ... The resulting solution was filtered through a 40 µm nylon mesh and treated with red blood cell (RBC) lysis (BioLegend). Blood was collected by cardiac puncture ...
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bioRxiv - Neuroscience 2020Quote: ... was carefully aspirated with a pipette and placed in 10mL of 1X solution of Red Blood Cell Lysis Buffer (BioLegend) for 15 minutes in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant was discarded and the pellet was resuspended in 10 ml of cold 1X Red Blood Cell Lysis buffer (Biolegend). Samples were vortexed and incubated in the dark for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with LIVE/DEAD Far Red viability for 20 minutes at RT and then with anti-CD32 (FITC, Biolegend) and anti-CD3 (PerCP ...
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bioRxiv - Immunology 2020Quote: ... Red blood cells were lysed with a hypotonic buffer and the cells surface stained for Ly6G (IA8 or RB6, Biolegend), CD11b (M1/70 ...
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bioRxiv - Immunology 2022Quote: ... The BM cells per animal were spined down and then resuspended in 1 mL Red Blood Cell Lysis (RBCL) buffer (Biolegend) at room temperature for 1 min to lyse the red blood cells ...
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bioRxiv - Systems Biology 2022Quote: ... The population corresponding to cluster B was sorted by top 1% of CD49c abundance via red florescent antibody (Biolegend 343808). Cells corresponding to cluster A were sorted from 25-75% abundance range ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... The resulting solution was filtered through a 40 µm nylon mesh and treated with red blood cell (RBC) lysis (BioLegend). Blood was collected by cardiac puncture ...
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bioRxiv - Immunology 2022Quote: ... Cells within each experimental donor group were pooled and labeled with green and red CFSE respectively according to manufacturer protocol (Biolegend). Cells were counted and mixed to produce a 1:1 ratio of green and red labeled cells ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood was collected via retro-orbital sinus sampling from mice anesthetized with inhalational isoflurane and red blood cells were lysed with RBC Lysis Buffer (BioLegend) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells were lysed by incubation with Red Blood Cell Lysis Buffer containing ammonium chloride for 15 minutes at 4°C (BioLegend). CD34+ selection was performed using AutoMACS cell separation technology following incubation with CD34+ selection beads (Miltenyi Biotech ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA and actin were stained with 1 μg/mL DAPI and phalloidin (1:1000, Flash Phalloidin™ Red 594; BioLegend). Testis were washed three times with PBS and mounted in (ProLong™ Gold Antifade Mountant ...
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bioRxiv - Immunology 2024Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Expression of cell surface markers in resting and CD3/CD28 activated cells was made by flow cytometry using fluorescent-coupled antibodies against CD4 (Biolegend, Cat: 100434), CD3 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Livers were sliced into 300μm thick sections on a vibratome and stained with Hoechst (1:2000) and directly conjugated fluorescent antibodies CD31 (Biolegend, 1:100, 390), CD8 (Biolegend ...
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Immunology 2023Quote: Biotinylated S1 protein was obtained commercially (BioLegend). A 4X molar excess was incubated with streptavidin-Alexa Fluor 647 (0.5 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... We split our single cell suspension in half and stained half of the uncultured HCI010 and HCI002 cells for FACS using fluorescent labeled antibodies for human specific CD298 (Biolegend, cat. no. 341704) and mouse-specific MHC-I (eBioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 30 min at 4°C with pre-conjugated fluorescent labeled antibodies with the following combinations: CD45 (BioLegend, 368532 (PE/Cyanine7)) ...
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bioRxiv - Cell Biology 2020Quote: ... Staining of the red blood cell membrane was performed with the anti-Ter119::Alexa647 antibody (Biolegend, 0.5 mg/ml; 1/1000) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Splenic cell suspensions were treated additionally with red blood cell lysis buffer at room temperature for 10 min (Sigma-Aldrichor BioLegend Inc) for removing RBCs to obtain single cell suspensions ...
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bioRxiv - Cancer Biology 2022Quote: ... The suspensions were then spun at 300 x g for 5 minutes and resuspended in red blood cell (RBC) lysis buffer (Biolegend, 420302) for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Spleens were dissected and dissociated by mashing over 100um filter and red blood cells were lysed using RBC lysis buffer (BioLegend 420301). Brain and spinal cords were dissected ...
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bioRxiv - Cell Biology 2023Quote: Blood was collected from mice via the retro-orbital sinus and red blood cells were lysed prior to staining with the following fluorochrome-conjugated antibodies: BV650 CD45.1 (BioLegend clone A20), AlexaFluor700 CD45.2 (BioLegend clone 104) ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellets were harvested and resuspended in 5 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... in phenol-red free DMEM-F12 in combination with live-dead stain Zombie NIR (1: 10,000 dilution; Biolegend, cat. no 423106) and incubated for 25 minutes at 37 °C and 5% CO2 conditions ...