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Citations for BioLegend :
401 - 450 of 5242 citations for Mouse WD repeat containing protein WRAP73 WRAP73 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated using MojoSort Mouse CD8 T Cell Isolation Kit (480035, BioLegend). CD8+ T cells were cultured and expanded in a similar fashion to γδ T cell populations ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by negative selection using the Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058). Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were then sorted by negative selection using Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058) and stained with 100 nM dichlorofluorescein diacetate (DCFDA ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were purified with the MojoSortTM mouse CD4 T cell isolation kit (Biolegend, 480006) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... The MojoSort Mouse Naïve CD4+ T cell Isolation Kit (#480040, BioLegend, San Diego, CA, USA) was used for the isolation of naïve CD4+ T cells as previously described (15) ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Tissue and serum samples were loaded directly onto ELISA plates according to protocol (Biolegend 439407 Legend Max Mouse IFN-B ELISA kit) ...
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bioRxiv - Immunology 2021Quote: ... The levels of IL-17 were measured by using ELISA MAXTM Deluxe Sets (BioLegend).
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bioRxiv - Immunology 2019Quote: ... Culture supernatants were collected and analyzed for IFNg and IL-2 by ELISA (BioLegend).
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bioRxiv - Bioengineering 2021Quote: ... IFNγ concentrations in cell culture supernatants were determined after 48h by ELISA (Biolegend # 430104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and analyzed by murine IFNg and IL-17A ELISA (BioLegend). Spleens were processed similar to the lungs without the need of enzymatic digestion.
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested 48 hours after culture and analyzed for IFNβ by ELISA (Biolegend) according to manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... supernatants were harvested and TNF and IL-2 levels were measured by ELISA (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: ... the resulting supernatant was harvested and used to quantify IL-10 by ELISA (Biolegend), or to test its capacity to block BV2 polarisation in the presence of E ...
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bioRxiv - Biochemistry 2020Quote: ... Human ucOCN measurements were performed using a commercially available human ucOCN ELISA (BioLegend, 446707) (Lacombe et al. ...
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bioRxiv - Microbiology 2021Quote: ... Secreted TNF-α was quantified in supernatants by enzyme-linked immunosorbent assay (ELISA; BioLegend).
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bioRxiv - Synthetic Biology 2022Quote: ... and IL-2 or IFNγ were measured by ELISA following the manufacturer’s protocol (BioLegend). Absorbances were measured with a Synergy H1 Hybrid Multi-Mode Reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... in PBS were added to 96-well high-bound Nunc MaxiSorp ELISA plates (BioLegend) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was used for triplicate samples in the ELISA (Biolegend, San Diego CA), carried out according to manufacturers instructions.
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bioRxiv - Immunology 2024Quote: IL-6 ELISA was performed according to the manufacturer’s instruction (Biolegend, Cat. No. 431301) with mouse serum diluted 1:50 ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Immunology 2021Quote: ... cells were purified using negative magnetic bead selection (MojoSort Mouse Pan Dendritic Cell Isolation Kit, BioLegend). 2.5×106 BMDCs per well were cultured for 6h in 6-well plates in RPMI complete (R10 ...
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bioRxiv - Cancer Biology 2022Quote: ... MCT-treated CD11b+ cells were isolated from tumors using MojoSort Mouse CD11b Selection Kit (BioLegend, 480109). For transfecting every million primary CD11b+ cells ...
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bioRxiv - Microbiology 2022Quote: Serum IFN-γ was assessed using a custom-made mouse panel 13-plex kit (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... with all incubations carried out in selection buffer using the MojoSort Mouse Ly6G Selection Kit (Biolegend), the EasySep Biotin Positive Selection Kit II (Stemcell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... cells were enriched from total splenocytes using the EasySep Mouse CD8+ T cell Isolation Kit (Biolegend) at day 3.5 post infection ...
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bioRxiv - Immunology 2023Quote: Cytokines were measured in serum using the Mouse Inflammation Panel LEGENDplex cytometric bead array kit (Biolegend). Data were analysed in FlowJo and analyte concentrations were determined using a sigmoidal 4PL standard curve in Prism (GraphPad).
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bioRxiv - Immunology 2024Quote: ... using the MojoSort magnet with MojoSort Mouse CD4 Naïve T Cell Isolation Kit (Cat.#480040 BioLegend). T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... the remaining volume was subjected to immunoprecipitation on protein A/G magnetic beads coated with 5 µg of mouse anti-HA IgG (Biolegend, clone 16B12) as described in the Magna RIP technical manual ...
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bioRxiv - Immunology 2019Quote: ... Culture supernatants were assayed in duplicate or triplicate using a human TNF ELISA MAX (Biolegend). In samples where undiluted supernatants fell outside the standard curve ...
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bioRxiv - Genomics 2020Quote: ... and IL-10 in culture supernatants were measured using ELISA Max Deluxe were from Biolegend® (CA ...
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bioRxiv - Neuroscience 2021Quote: ... then analyzed for IL-1β using an IL-1β ELISA (BioLegend, 437004, San Diego CA) according to the manufacturer protocol.
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bioRxiv - Cell Biology 2021Quote: ... were taken on day 1 and 3 and analyzed for TGFβ using ELISA assay (BioLegend) and IFNγ and TNFα using a multiplex-bead based assay (Meso Scale Discovery ...
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bioRxiv - Immunology 2021Quote: ... IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems). For data output cell number and ELISA were normalized per 1,000 of each APC subset used for co-culture.
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bioRxiv - Immunology 2021Quote: ... and supernatants were harvested for IL-6 and IL-1β ELISA (Biolegend, San Diego, CA). BMDCs were also utilized in the MLR and in vitro T cell differentiation assays.
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bioRxiv - Immunology 2023Quote: ... and cytokines (IFN-y and IL-5) were measured by ELISA (Biolegend. San Diego, CA). For intracellular cytokine determination ...
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bioRxiv - Synthetic Biology 2023Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFNɣ by ELISA (BioLegend).
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bioRxiv - Cancer Biology 2023Quote: ... The supernatants collected were diluted 1:1000 prior to the IFNψ ELISA assay (BioLegend, 430104). The same co-culture procedure was performed in parallel with wildtype CD8 T cells from the same donor as a control.
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... ELISA assay to measure VEGFA levels was performed according to the manufacturers’ protocol (Biolegend, 430204) using the lung cancer cell supernatant.
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bioRxiv - Cancer Biology 2023Quote: ... Comprehensive surface immunophenotyping was performed using LEGENDScreen Human PE Kit containing 371 pre-titrated antibodies and 354 unique specificities (BioLegend). The experimental run was multiplexed using GFP+ NCI-H358 cells resistant to sotorasib combined with non-fluorescent ...
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bioRxiv - Immunology 2022Quote: ... prior to incubation with staining antibodies (PE/Cy7 anti-mouse CD45, BV711 anti-mouse CD3, BV421 anti-mouse CD19, FITC anti-mouse CD31, and APC anti-mouse podoplanin, all BioLegend) and one Hashtag antibody per replicate using BioLegend TotalSeq-B03 (catalog numbers 155831 ...
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bioRxiv - Cell Biology 2021Quote: ... purified mouse anti mouse/human CD207 (Biolegend, 144202), rabbit anti-mouse/human Ki67 (abcam ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal against mouse β-III-tubulin (Biolegend); mouse monoclonal against HA (12CA5 ...