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Citations for BioLegend :
401 - 450 of 5080 citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Protein levels in plasma and skin tissue protein extracts were measured using defined analyte panels: Legendplex Mouse Inflammation Panel and Inflammatory Chemokine Panel (Biolegend).
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2022Quote: ... endogenous biotin and avidin proteins were successively blocked using an Avidin/Biotin Blocking Kit (Biolegend), for 10 min each ...
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bioRxiv - Immunology 2020Quote: ... The cells were activated in 96-well round bottom plates that were coated with 3ul/ml anti-CD3e (BioLegend #100202) and 5ul/ml anti-CD28 (BioLegend #102102 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Cell Biology 2021Quote: ... ESLAM Sca-1+ cells were isolated by fluorescence-activated cell sorting (FACS) as previously described (Kent et al., 2009) using CD45 BV421 (Clone 30-F11, Biolegend), CD150 PE/Cy7 (Clone TC15-12F12.2 ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were activated for 24h using 2 μg/ml of anti-Cd28 and anti-Cd3ε antibodies (102116 and 100340, BioLegend).
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bioRxiv - Immunology 2020Quote: ... lymph nodes were removed from Myc-eGFP mice and mashed and cell suspension activated with anti-CD3 (1 µg/mL, clone 145-2C11, Biolegend) and anti-CD28 (3 µg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Activated CD4+ and CD8+ T cells were stimulated with a cell activation cocktail (Biolegend; CA PMA/Ionomycin with Brefeldin A) overnight and stained for IFN-γ and TNF-α after fixation and permeabilization using the eBioscience/Thermo kit (Grand Island ...
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bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... We stably expressed these plasmids in OCI-Ly8 cells and used fluorescence activated cell sorting (FACS) to enrich for cells that stained negative with PE anti-human CD20 antibodies (clone 2H7, Biolegend). This sorted CD20-negative cell population were the OCI-Ly8 CD20KO cells.
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were activated in 96-wells culture plates (25 x103 cells/well) coated with anti-CD3 and anti-CD28 (Biolegend) or by the addition of the DPP-8/9 inhibitor Talabostat (Val-boroPro ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Immunology 2024Quote: γδ IELs were cultured ex vivo as previously described (33) in which 100,000 γδ IELs were activated with 1 ÿg/mL anti-CD3 (2C11, BioLegend) in the presence of 10 U/mL IL-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... and plasma were assayed for CXCL5 using a human/mouse CXCL5 immunoassay kit (BioLegend, 440904).
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bioRxiv - Developmental Biology 2019Quote: ... placental single-cell suspensions were stained with APC-conjugated anti-mouse CD117 (c-kit) (BioLegend), PerCP/Cy5.5-conjugated anti-mouse CD34 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: All MPC experiments in this protocol were performed with Biolegend LEGENDScreen Mouse PE Kit (Biolegend) and manufacturer recommendations for LEGENDScreen Plate preparation and staining were performed as indicated by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... 13) were assayed using LEGEND plex Mouse Th1/Th2 panel kits (BioLegend, San Diego, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by negative selection using the Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058). Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were then sorted by negative selection using Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058) and stained with 100 nM dichlorofluorescein diacetate (DCFDA ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated using MojoSort Mouse CD8 T Cell Isolation Kit (480035, BioLegend). CD8+ T cells were cultured and expanded in a similar fashion to γδ T cell populations ...
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bioRxiv - Immunology 2024Quote: ... The MojoSort Mouse Naïve CD4+ T cell Isolation Kit (#480040, BioLegend, San Diego, CA, USA) was used for the isolation of naïve CD4+ T cells as previously described (15) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were purified with the MojoSortTM mouse CD4 T cell isolation kit (Biolegend, 480006) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Immunology 2022Quote: ... Tissue and serum samples were loaded directly onto ELISA plates according to protocol (Biolegend 439407 Legend Max Mouse IFN-B ELISA kit) ...
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bioRxiv - Immunology 2021Quote: ... The levels of IL-17 were measured by using ELISA MAXTM Deluxe Sets (BioLegend).
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bioRxiv - Immunology 2019Quote: ... Culture supernatants were collected and analyzed for IFNg and IL-2 by ELISA (BioLegend).
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bioRxiv - Bioengineering 2021Quote: ... IFNγ concentrations in cell culture supernatants were determined after 48h by ELISA (Biolegend # 430104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and analyzed by murine IFNg and IL-17A ELISA (BioLegend). Spleens were processed similar to the lungs without the need of enzymatic digestion.
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested 48 hours after culture and analyzed for IFNβ by ELISA (Biolegend) according to manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... supernatants were harvested and TNF and IL-2 levels were measured by ELISA (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: ... the resulting supernatant was harvested and used to quantify IL-10 by ELISA (Biolegend), or to test its capacity to block BV2 polarisation in the presence of E ...
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bioRxiv - Biochemistry 2020Quote: ... Human ucOCN measurements were performed using a commercially available human ucOCN ELISA (BioLegend, 446707) (Lacombe et al. ...
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bioRxiv - Microbiology 2021Quote: ... Secreted TNF-α was quantified in supernatants by enzyme-linked immunosorbent assay (ELISA; BioLegend).
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bioRxiv - Synthetic Biology 2022Quote: ... and IL-2 or IFNγ were measured by ELISA following the manufacturer’s protocol (BioLegend). Absorbances were measured with a Synergy H1 Hybrid Multi-Mode Reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... in PBS were added to 96-well high-bound Nunc MaxiSorp ELISA plates (BioLegend) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was used for triplicate samples in the ELISA (Biolegend, San Diego CA), carried out according to manufacturers instructions.
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bioRxiv - Immunology 2024Quote: IL-6 ELISA was performed according to the manufacturer’s instruction (Biolegend, Cat. No. 431301) with mouse serum diluted 1:50 ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Final volumes of 500 μl resuspended cells were placed on ice and sorted by fluorescence activated cell sorting (FACS) to isolate live cells that excluded the cytoplasmic stain Zombie green (BioLegend, 423111). For scRNAseq library construction ...
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bioRxiv - Immunology 2021Quote: 600 million cells from human tonsils were resuspended in Miltenyi magnetic-activated cell sorting (MACS) buffer and stained with biotinylated anti-human CD19 (clone: HIB19; BioLegend, #555411) on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Immunology 2021Quote: ... cells were purified using negative magnetic bead selection (MojoSort Mouse Pan Dendritic Cell Isolation Kit, BioLegend). 2.5×106 BMDCs per well were cultured for 6h in 6-well plates in RPMI complete (R10 ...
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bioRxiv - Cancer Biology 2022Quote: ... MCT-treated CD11b+ cells were isolated from tumors using MojoSort Mouse CD11b Selection Kit (BioLegend, 480109). For transfecting every million primary CD11b+ cells ...
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bioRxiv - Microbiology 2022Quote: Serum IFN-γ was assessed using a custom-made mouse panel 13-plex kit (LEGENDplex, BioLegend) following the manufacturer’s instructions ...