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Citations for BioLegend :
351 - 400 of 5029 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained for 20-30 min at 4°C with an antibody mix composed of: Zombie UV viability dye (BioLegend), anti-CD45RA (Beckman) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized using BD Fix/Perm for 20 min at RT and stained intracellularly with anti-IFN-γ (1:100 dilution; clone XMG1.2, Biolegend), anti-TNF-α (1:100 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Cell Biology 2023Quote: ... injection of 500 mg/kg), and vascular markers (20 µg) of interest conjugated to AF647 (CD31, ICAM1, ICAM2, and CD36 (BioLegend), P-selectin (BD Pharmingen)) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with FACs buffer (1× PBS, 1% FBS) and fixed at RT for 20 min with Intracellular Staining Fixation Buffer (BioLegend). Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled stained in PBS for 20 mins at 4°C with a live dead marker (Zombie UVTM, Biolegend). Surface staining was performed in brilliant stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... The samples were then fixed for 20 min at room temperature in the dark with 100 μL of fixation buffer (BioLegend). For surface staining only ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... CD4+ and CD8+ T cells from PBMC were stained with the following cell surface markers in MACS buffer at room temperature for 20 mins: CD3-AF488 (clone OKT3, Biolegend), CD14-eFluor450 (clone 61D3 ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... Alexa Fluor 488 anti-mouse CD117 (c-Kit) (Biolegend, 105816), PE/Dazzle-594 anti-mouse Ly-6A/E (Sca-1 ...
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bioRxiv - Bioengineering 2021Quote: ... and Legend Max Mouse CXCL13 (BLC) ELISA kit (BioLegend, 441907) were used to quantify different serum cytokines ...
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bioRxiv - Cell Biology 2021Quote: ... MojoSort mouse CD4 T cell isolation kit was from BioLegend. Liberase TL (Research Grade ...
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bioRxiv - Molecular Biology 2024Quote: ... and PE/Cyanine7 anti-mouse CD117 (c-kit) (Biolegend 135112), at the appropriate concentrations for the volume and cell count (1uL in 1 million cells) ...
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bioRxiv - Genetics 2021Quote: ... production from LPS- or OL-treated BMDM were performed using Mouse TNF-α ELISA MAX Deluxe kit and Mouse IL-6 ELISA MAX Deluxe kit (BioLegend, San Diego, CA, USA). Follow-up cytokine (IL1β ...
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... PMNs and platelets were visualized by applying 20 μl of platelet-specific FITC-labeled X488 antibody (Emfret, Eibelstadt, Germany) and 20 µl of PE-labeled anti-Ly6G (BioLegend #127608) with a catheter ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Molecular Biology 2022Quote: ... an equal number of cells from each group were incubated at room temperature for 20 minutes with Zombie Aqua™ Fixable Viability dye (BioLegend) and blocked on ice for 20 minutes with FcBlock anti-CD16/CD32 (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse and human cells were isolated as described above and stained with Zombie fixable viability dye for 20 min at room temperature (#423102, BioLegend, USA) and pre-incubated with anti-mouse Fc block at 10 μg mL-1 (#101320 ...
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bioRxiv - Cancer Biology 2023Quote: ... a fixation/permeabilization step was performed by incubating the cells for 20 min at RT using a Cyto-Fast Fix/Perm Buffer set (#426803, BioLegend, USA). Intracellular antibody staining was then performed for 25 min at RT ...
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bioRxiv - Bioengineering 2023Quote: Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend, 368534) and anti-IFN-γ (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in 1X PBS and stained with Alexa Fluor 700 anti-human B2M antibody (BioLegend, 395708; 1:20 dilution) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: High-binding 96-well flat-bottom plates were coated with 100 μl per well of 20 μg/ml anti-NK1.1 (Clone PK136, BioLegend, cat. 108759) in PBS at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then stainied with the follow antibodies for 20 min on ice: anti-CD45 (APC-Fire 750, 30-F11, BioLegend 103153), anti-CD3 (BV785 ...
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bioRxiv - Genetics 2024Quote: ... Another 400 μL of supernatant was mixed with 600 μL of FSPP buffer and 20 μL of pre-blocked Magnetic IgG beads and TAP antibody (Thermo CAB1001) or Rpb3 antibody (Biolegend 920204) for 10–12 hrs at 4°C ...
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bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated using the MojoSort Mouse Ly6G Selection Kit (Biolegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... ELISA kits for detection of mouse IL-1β was from BioLegend and for mouse IL-12p70 and human IL-1β were from BD ...
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bioRxiv - Immunology 2022Quote: ... and LEGEND MAX™ Mouse IL-35 Heterodimer ELISA Kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The MojoSort Mouse Naïve CD4+ T cell Isolation Kit (#480040, BioLegend), anti-CD3ε antibody (Ab ...
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... slices were incubated with 20 μg/ml anti-B220 antibodies (AF647-αB220 Rat IgG2a, κ or FITC-αB220 Rat IgG2a, κ, BioLegend) at 37 °C for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Cell surface proteins were stained for 20 min at 4 °C with the following antibodies: CD45.1 (BioLegend, 110714, Clone ID: A20, RRID:AB_313503), CD45.2 (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 20– 30 min in the dark on ice with the conjugated antibodies as follows (BioLegend unless denoted otherwise) following manufacturer’s recommended concentrations ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were resuspended in 0.1% Tween-20 in PBS supplemented with 5% BSA at the following dilutions: TUJ1 (BioLegend, 801202, 1:400), SOX2 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... for 20 minutes at 4°C and stained in perm wash buffer with anti-IFNg PE or BV785 (ThermoFisher or BioLegend, clone: XMG1.2) for one hour at room temperature.
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...