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Citations for BioLegend :
451 - 500 of 854 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... or restimulated on day 11 in 96-well plates with 2.5ug/ml recombinant SARS-CoV-S protein S1 (Biolegend 792906, carrier-free) for 8h in the presence of 1ug/ml Golgiplug and 0.7ug/ml Golgistop ...
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bioRxiv - Immunology 2020Quote: ... after which Fc receptors were blocked using TruStain FcX (anti-mouse CD16/32, Biolegend). Cell surface staining was performed at room temperature (for details of antibodies and panels refer to Additional File 1 ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Fc receptors were blocked using TruStain FcX™ (anti-mouse CD16/32) Antibody (Biolegend, Cat# 101320 ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 cells/sample were Fc-blocked with TruStain fcX (BioLegend, #101320, 1/200) for 10 minutes at room temperature followed by LIVE/DEAD Fixable Aqua (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... and stained with an AF647-labeled anti-human IgG Fc antibody (Biolegend, catalog 409320). Cells were then analyzed by flow cytometry on LSRFortessa analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with TruStain FcX anti-mouse CD16/32 Fc block (Biolegend) for 10 min on ice in Cell Stain Buffer (Biolegend) ...
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bioRxiv - Cancer Biology 2021Quote: Cells were pre-treated with murine Fc block (TruStain FcX, BioLegend, San Diego, CA,) at room temperature for 15 minutes then incubated with antibodies Gr1 [RB6-8C5] ...
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in 1:50 dilution of human FC blocking solution (BioLegend; #422302) and incubated on ice for 10 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were surface stained with fixable viability dye (Thermo-Fisher) and Fc-Block (Biolegend), then fixed in 2% PFA for 15 minutes followed by permeabilization and staining with fluorochrome-conjugated antibodies in staining buffer containing 0.5% saponin ...
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bioRxiv - Immunology 2020Quote: ... Digested crypts cells were incubated with anti-mouse TruStain Fc (1:400, BioLegend 101320) for 5 minutes in ice ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with anti-mouse CD16/32 Fc receptor block (clone 93, Biolegend) for 15-30 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with anti-mouse CD16/32 Fc receptor block (clone 93, Biolegend) for 15-30 minutes ...
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bioRxiv - Genomics 2021Quote: ... Fc receptor block from two vendors (TruStain FcX, BioLegend and FcR blocking reagent, Miltenyi) was added to the suspension and incubated for 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... non-specific binding was blocked using Fc Receptor blocking solution (Human TruStain FcX, BioLegend) and cells were stained with antibodies in PBS supplemented with 2% hiFCS for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: After blockade of Fc-receptors using Human TruStain FcX™ (BioLegend, London, United Kingdom), 5*106 PBMCs were stained with fluorescently labelled antibodies in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... re-suspended and labelled with Fc block (Human TruStain FcX™; Biolegend, London, UK) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc-receptor blocked and stained with anti-mouse CD11b (clone M1/70, BV650, BioLegend) and anti-mouse CD45 (clone 30-F11 ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc-receptor blocked and stained with anti-mouse CD11b (clone M1/70, FITC, BioLegend), anti-mouse CD45 (clone 30-F11 ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2022Quote: ... 1 million cells were blocked with TruStain Fc Block (anti-mouse CD16/32, Biolegend). Cells were stained for 30 mins with antibodies specific to CD45-FITC (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked by adding 2.5 μL Human TruStain™ FcX (BioLegend, #101320) per 50 μL of 200 000 cells ...
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bioRxiv - Microbiology 2022Quote: ... Single-cell suspensions were preincubated with Fc block antibody (1:100; clone S17011E; Biolegend) in PBS + 2% heat-inactivated FBS + 1 mM EDTA for 20 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then stained with anti-human IgG Fc-APC (Biolegend, Cat#366906) on ice for 30 min and washed with PBS twice ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with Fc Block anti-Mouse CD16/CD32 (1:200, 101301, Biolegend) for 10min at RT and then stained for intracellular TNFα with the BD Fixation & Permeabilization kit according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with Fixable Viability Dye (eBioscience) and incubated with Fc Block (Biolegend) prior to staining with antibodies against surface antigens (CD45 ...
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bioRxiv - Developmental Biology 2023Quote: ... Non-specific bindings were blocked using Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend) for 10 min on ice in the dark ...
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bioRxiv - Physiology 2023Quote: ... cells were resuspended in 5% FBS/PBS with Fc-blocking antibody (TruStain FcX; BioLegend) at 0.01 mg/ml for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... after saturation of non-specific sites with Humain TruStain FCX FC Blocking Reagent (Biolegend). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 5 µl of the Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend, #422302) were added to the sample and incubated for 10 min on ice ...
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bioRxiv - Immunology 2023Quote: ... The single cell suspension was blocked in FC-block CD16/32 (1:100, Biolegend) and subsequently incubated in Zombie Aqua viability stain (1:200 ...
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bioRxiv - Immunology 2024Quote: ... following Fc receptor saturation (CD16/32, clone 93, dilution 1:50 in PBS, Biolegend) and dead cell detection with Fixable Viability Stain 440UV (dilution 1:500 in PBS ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... HA.11 Epitope Tag Antibody (1:1000, 901513) was from BioLegend (previously Covance catalog# MMS-101R). V5-Tag (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... using mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:1,000 dilution), mouse anti-β-actin monoclonal (clone AC-74 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with a rabbit antibody against the C-myc epitope tag (1:1000, BioLegend) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... washed and stained with Tag-it Violet™ Proliferation and Cell Tracking Dye (Biolegend Cat.425101) or CellTrace™ Far Red Cell Proliferation Kit (Invitrogen Cat.C34564) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast were then incubated with 0.5 μg of Alexa Fluor 488-labelled HA tag antibody (Biolegend) for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were stained with 2.5 µM Violet Tag-It dye (BioLegend, San Diego, CA, USA) for 15 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... FITC-conjugated anti-Human IgG Fc antibody (clone M1310G05, BioLegend 410719, San Diego, CA, USA) was used to detect bound anti-CTLA-4 ...
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bioRxiv - Systems Biology 2021Quote: ... for 5 min to block Fc receptors and then stained with antibodies against CD11b (BioLegend), Ly6C (BioLegend) ...
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bioRxiv - Physiology 2022Quote: ... Cells were then pre-incubated with mouse-Fc block and viability dye (Zombie Aqua, BioLegend) in calcium/magnesium-free PBS (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and stained with 1.25 µg/mL anti-human IgG Fc-PE/Cy7 (Clone HP6017; Biolegend) and 5 µg/mL anti-flag-FITC (clone M2 ...
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bioRxiv - Genetics 2019Quote: ... at ∼1M cells/100μl and incubated with anti-mouse CD16/CD32 Fc Block (BioLegend # 101319) at room temperature for 10-15 min ...
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bioRxiv - Microbiology 2021Quote: THP-1 human macrophages were treated with mouse anti-human Fc block (Ref: 422302, BioLegend), for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Cells were surface stained with mAbs diluted in PBS/0.1% BSA and Fc-block (Biolegend) for 30 min on ice ...
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bioRxiv - Pathology 2022Quote: ... human and murine Fc receptors were blocked using Human TruStain FcX (Biolegend, #422302, 1/20) and anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with PE labeled anti-human IgG Fc secondary antibody (Biolegend) at a 1:20 dilution in 50 μl staining buffer at room temperature for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for >5 minutes at 4C with Fc Block (clone 93; BioLegend), then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies ...