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Citations for BioLegend :
1 - 50 of 1250 citations for L Valine 13C5 95 97%; 15N 96 99%; 2 3 D2 97%+ since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Mbp (RRID:AB_2564741, 1:500, Biolegend, SMI-99,), SMI312 (RRID:AB_2135329 ...
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bioRxiv - Neuroscience 2020Quote: ... Mbp (1:500, RRID:AB_2564741, #SMI-99, Biolegend), TCF7l2 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-mouse Mac-2 (Galectin-3, 125402, Biolegend) for myeloid cells (Ho and Springer 1982) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 or 3 (TotalSeq-A, clone LNH-94, Biolegend) for GoT-IM ...
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bioRxiv - Genetics 2021Quote: ... mouse anti-MBP (1:4.000; clone SMI 94/SMI 99, BioLegend); rabbit anti-MAG (1:1.000 ...
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bioRxiv - Immunology 2019Quote: ... Antibodies include mouse anti-human ataxin-3 65042 1H9-2 (BioLegend), mouse anti-human MIC60 ab110329 (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies used in western blot are MBP (SMI-99, RRID:AB_2564741, 1:2000, Biolegend), Active β-catenin (#05665 ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Immunology 2023Quote: ... 2/3 of the cells were fixed with 750μL fixation buffer (BioLegend, #420801) for 30min ...
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Immunology 2024Quote: ... and penicillin in a 96-well plate pre-coated with anti-CD3Ab (2 μg/ml, Biolegend 100360), along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122 ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2022Quote: A 3-plex SARS-CoV-2 Serological IgG Panel (BioLegend LEGENDplex, Cat. No. #741132) was used for measuring IgG titre of the 3 key COVID-19 coronavirus derived proteins ...
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bioRxiv - Immunology 2024Quote: ... with 3 µg of APC-labeled anti-CD8b.2 antibody (clone 53-5.8, Biolegend) 5 min prior to euthanasia to mark vasculature-associated cells in vivo.
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2023Quote: ... The nuclei were stained with 3 µM DAPI (4’,6-diamidino-2-phenylindole; BioLegend, #422801) for 5 min ...
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bioRxiv - Immunology 2019Quote: ... 96-well plates (Biolegend) were pre-coated with 100 µl/well of 7×104 PFU of X31 virus in PBS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... infected or stimulated cells using a LEGENDplex Human Type 1/2/3 Interferon Panel assay (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... CD8 T cells were >99% pure and assessed by flow cytometry staining with CD8-Pacific Blue antibody (Biolegend). Total CD8 T cells were activated using anti-CD3/CD28 coated beads (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... 2.1 mmol/L ultra-glutamine in the presence of Brefeldin A (5 μg/ml) and monensin (2 μM; BioLegend). After 5 hours of incubation ...
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bioRxiv - Physiology 2022Quote: ... Samples were stained with 3 μM of (4’,6-Diamidino-2-Phenylindole, Dilactate (DAPI, Biolegend Cat# 422801)) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Immunology 2022Quote: 96-well ELISA plates (BioLegend) were coated with 50 μL of Ovalbumin from chicken egg white (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Lower compartment of the transwell plate was loaded with 200 µl of X-VIVO-15 supplemented with 2 % HS and 100 ng/ml of CCL19 and CCL21 (#582102, #582202, Biolegend). To determine passive cDC2 migration ...
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bioRxiv - Immunology 2020Quote: ... Tim-3 clone RMT 3-23 (BioLegend) and clone FAB1529 (R&D) ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Immunology 2020Quote: ... The purity of the cells was 99 % as analyzed by flow cytometry using the macrophage markers CD11b (M1/70, BioLegend) and F4/80 (BM8 ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Immunology 2021Quote: ... or isotype control antibodies (clone R35-95, 553930, BD Pharmingen; clone HTK888, 400924, BioLegend; clone RTK4530, 400611, BioLegend), fixed with 2% PFA and analysed on a BD LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... or isotype control antibodies (clone R35-95, 553930, BD Pharmingen; clone HTK888, 400924, BioLegend; clone RTK4530, 400611, BioLegend), fixed with 2% PFA and analysed on a BD LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... αPD-l-BV421 (Biolegend, #109121) and αCD39-APC (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...