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Citations for BioLegend :
401 - 450 of 1373 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human TCR α/β antibody (IP26: Biolegend), HLA.A2/9V-AF647 or CD19-AF647 to determine the concentration of the staining agent leading to label saturation for CMV-specific RA14 TCR T-cells ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Live naïve CD4+ T cells were purified from PBMCs post staining with fluorescence labelled antibodies to human CD3(OKT3, Biolegend), CD4 (RPA-T4 ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... CD4+ OT-II KN2 cells were purified from CD45.1.2 OT-II KN2 donor mice with the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend) and labeled with cell proliferation dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Biochemistry 2023Quote: ... Naïve CD4+ T cells were then activated with anti-CD3 (Biolegend, Cat#100340, 1:500 dilution to 5μg/mL), anti-CD28 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Neuroscience 2024Quote: ... Several positive controls were utilised: (1) polyclonal T-cell simulation with soluble αCD3 (0.5 μg/ml; clone OKT3; Biolegend) and α-CD28 (1μg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a T cell activation analysis cocktail including: anti-mouse CD3 clone 17A2 APC conjugate (BioLegend, 100235), rat anti-mouse CD4 AlexaFluor700 conjugate (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... IFN-γ secretion to validate CAR-T cell activation was measured using the Human INF-γ ELISA kit (Biolegend) or Human IFN-γ DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated for 3 days using 3 μg anti-human CD3 and CD28 antibodies (Biolegend, 300438, 377604) and 50 ng/mL of IL-2 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells (4X105/well in 96-well plate) were activated with plate-coated αCD3 (5µg/ml, Biolegend) and αCD28 (10µg/ml ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Cell Biology 2020Quote: ... Diamidino-2-Phenylindole (DAPI) (Biolegend) for live/dead exclusion and cell sorting ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (MQ1-17H12, Biolegend), and IFNγ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... and 2 μM monensin (BioLegend). For intracellular staining of IgM ...
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bioRxiv - Cancer Biology 2022Quote: ... and monensin (2 μM, Biolegend) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... beta-2-microglobulin (BioLegend #316306) and Fixable Viability Dye eFluor780 or LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher #65-0865-14 1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... and 2 nM Monensin (BioLegend) were added to each well and incubated for further 2 h ...
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bioRxiv - Immunology 2019Quote: ... CD138(BV421, 281-2, BioLegend, #142507 ...
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bioRxiv - Immunology 2020Quote: ... anti-CD138 (281-2, Biolegend), anti-IgD (11-26c.2a ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... 2) huCD45 APC Cy7 (BioLegend), huCD3 PerCp-Cy5.5 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... CD138 (clone 281-2, Biolegend), B220 (clone RA3-6B2 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD138 (BioLegend; clone: 281-2) and analyzed for germinal center activated B cells (GCB) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 PE (Biolegend 503808), CD4 PE-Cy7 (Biolegend 100528) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 BV605 (Biolegend 500332), and Live/dead Near-IR (Invitrogen L10119) ...
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bioRxiv - Immunology 2021Quote: ... or -BV510 (281-2, Biolegend) and B220-BV421 (RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... CD49d-FITC (R1-2, BioLegend), Tbet-APC (4B10 ...
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bioRxiv - Pathology 2023Quote: ... CD206 (Biolegend; clone 15-2) and CD163 (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... mouse CD63 (NVG-2, Biolegend), CD9 (312102 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (JES6-5H4; Biolegend), TNFa-BV605 (MP6-XT22 ...
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bioRxiv - Bioengineering 2023Quote: ... IL-2 (BioLegend, Cat. # 500326), and Perforin (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and monensin (2 μM, Biolegend) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and CADM1 (2:100, BioLegend) at 37°C for 25 min ...
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bioRxiv - Immunology 2023Quote: ... anti-CD63 (NVG-2, Biolegend) and anti-XCR1-AF647 (ZET ...
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bioRxiv - Immunology 2023Quote: ... and CD138 (281-2, BioLegend), while human cells were incubated with CD20 (2H7 ...
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bioRxiv - Immunology 2024Quote: ... and monensin (2 µM; Biolegend). Phorbol 12-myristate13-acetate (PMA)/ionomycin-stimulated (Cell Activation cocktail ...
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bioRxiv - Immunology 2023Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ CD25- and CD8+ T cells were purified using the MojoSort Mouse CD4 (supplemented with biotinylated anti-CD25) and CD8 T Cell Isolation Kits (BioLegend), respectively ...
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bioRxiv - Immunology 2022Quote: We quantified CD4+ and CD8+ T cells by incubating 100 μl of whole blood with anti-CD8-Brilliant Violet 421 (clone SK1; BioLegend), anti-CD4-APC (clone L200 ...
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bioRxiv - Immunology 2021Quote: Negative selection of CD4+ T cells from naïve splenocytes was carried out using the MojoSort™ magnetic cell separation system (Biolegend). CD4+ T cells were re-suspended in in RPMI 1640 supplemented with 10% FCS ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used for coating of culture plates for in vitro T cell activation: anti-human CD3 (clone HIT3α; Biolegend), anti-human CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel Parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... cells were re-suspended in RPMI1640 growth media supplemented with T cell stimulation cocktail (4975-03, eBioscience Inc., San Diego, CA) and brefeldin A (Biolegend) and incubated at 37°C for 4 hr.
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bioRxiv - Cancer Biology 2022Quote: ... the phenotype of live T-/CAR T-lymphocytes in all the experimental conditions was assessed using the following antibodies: BV510-anti CD3 (clone OKT3, BioLegend), APC-CY7-anti CD3 (clone UCHT1 ...