Labshake search
Citations for BioLegend :
301 - 350 of 5776 citations for Interleukin 10 Receptor Alpha IL10RA Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Tim-3 was either neutralized by anti-human Tim-3 antibodies (10 µg/mL) (Biolegend) for 1 h prior to treatment or through knockdown via duplexed siRNA (Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... cells were pre-incubated with 10 ug/mL anti-SIGLEC1 antibody (Biolegend, clone 7-239) for 30 min ...
-
bioRxiv - Immunology 2021Quote: ... GD2 was blocked by staining with 10 ug/mL anti-GD2 antibody (clone 14G2a; BioLegend) for 30 minutes on ice ...
-
bioRxiv - Microbiology 2021Quote: ... and a non-PfEMP1-blocking anti-ICAM-1 antibody (Biolegend Cat # 322702, 10 μl/ml) as negative controls ...
-
bioRxiv - Cancer Biology 2023Quote: ... then blocked with anti-CD16/32 purified antibody at 1:100 for 10 mins (Biolegend). Samples were stained with directly conjugated antibodies (see below ...
-
bioRxiv - Cell Biology 2023Quote: ... as well as 0.5 ug each of the 10 TotalSeq-B oligo-tagged antibodies (BioLegend) listed in Supplementary Table 3 were added and cell pellets were suspended ...
-
bioRxiv - Immunology 2024Quote: ... and FcεRIγ (FCABS400F antibody, Milli-pore)10 protein expression on live (Zombie red, 77475; BioLegend), CD3-negative (300318 antibody ...
-
bioRxiv - Biophysics 2023Quote: ... we incubate 5-6*105 NK92 cells prior to mixing them into the collagen for one hour in serum-free cell culture medium at 37°C with or without the following antibodies: 10 μg/ml anti-integrin activating β1 (CD29) antibody (Biolegend, cat. no. 303002) and the corresponding IgG1 isotype (Biolegend ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
-
bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
-
bioRxiv - Immunology 2022Quote: ... Cells were stained with a staining mix containing Fc receptor blocker (TruStain fcX™, BioLegend, Amsterdam, The Netherlands, 1:100), viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit ...
-
bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
-
bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
-
bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
-
bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 μl of FACS Buffer and incubated with 1 μl of allophycocyanin (APC)-conjugated anti-CD46 antibody (BioLegend, clone TRA-2–10, 352405) for 30 min at 4°C with rotation ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were incubated for 10 min using unconjugated anti-mouse CD16/32 antibody (Biolegend, C93, #101301) at 4°C prior to labelling with specific fluorochrome-labelled antibodies ...
-
bioRxiv - Physiology 2021Quote: ... an antibody directed against CD45-BV605 (clone 30-F11, 1:10 in 100 μl PBS, BioLegend) was injected intravenously 5 min before euthanizing the animals ...
-
bioRxiv - Immunology 2022Quote: ... A second staining with 10 μg/ml IgG2b mouse monoclonal antibody 9C4 directed against EpCam (Biolegend) allowed the identification of epithelial cells by indirect detection with anti-IgG2b Cyanine-3 labelled secondary Ab (Jackson ImmunoScience ...
-
Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... Ultra-LEAF™ purified anti-human CD95 (FAS) blocking antibody (10 μg/ml, clone A16086F, Biolegend) or respective DMSO and H2O control were added prior to incubation ...
-
bioRxiv - Immunology 2020Quote: ... wells were supplemented with 10 μg/ml of αLFA1 antibody (Clone: TS 1/18, BioLegend, 302116) or with 10 μg/ml of αFAS-L antibody (BD ...
-
bioRxiv - Immunology 2020Quote: ... CD45 was labeled with 10 nM anti-CD45 antibody (clone 30-F11 Brilliant Violet 421, BioLegend).
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 antihuman CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
-
bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 anti-human CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
-
bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend, 343606 at 1.25 µg/mL).
-
bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were anti-tau monoclonal antibodies including DA9 (aa 102–140, 1:10 dilution), ET3 (aa 273-288, 1:10 dilution)57 and 77G7 (BioLegend, aa 316-355, 1:100 dilution). DA9 and ET3 antibodies were generously provided by Peter Davies ...
-
bioRxiv - Cancer Biology 2022Quote: ... and then plated in a culture plate pre-coated with 10 µg/mL anti-CD3Ɛ antibody (Biolegend). Culture medium was exchanged to R10 containing IL-2 and the cell density was adjusted to be 0.5 – 1x106 cells/ml every 24 h.
-
bioRxiv - Physiology 2022Quote: ... mice were intravenously injected with 10 μg of Alexa Fluor 647 anti-mouse CD144 antibody (BV13, BioLegend) and euthanized 10 minutes after injection ...
-
bioRxiv - Immunology 2021Quote: ... Secondary antibodies used in these studies against IL 10 (#501501) and IL-7 (#506601) were from Biolegend, and against IFN-γ (#MAB285) ...
-
bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
-
bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat: 324420; anti-EpCAM-AF647, Biolegend, Cat: 118211) at 4 ºC for >30 min ...
-
bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
-
bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
-
bioRxiv - Immunology 2020Quote: ... 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of IL-10-BV421 antibody (Biolegend, 501421) was added for an additional 20 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... neurons were incubated live with 10 µg/ml of β1-integrin antibody clone HM β1-1 (102202, Biolegend) for 15 min at 37 °C ...