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Citations for BioLegend :
301 - 350 of 3719 citations for IL 4RA CD124 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Immunology 2021Quote: ... IL-33 (Biolegend) or 20 μg of NMU (US Biological ...
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bioRxiv - Bioengineering 2020Quote: ... IL-6 (Biolegend) and TGF-β (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... IL-22 (Biolegend), mouse TGFβ1 (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: ... IL-5 (BioLegend) and IL-13 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... IL-17A (BioLegend). For the detection of intracellular IL-17A expression ...
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bioRxiv - Immunology 2021Quote: ... IL-17 (Biolegend) and CCL17 (R&D Systems) ...
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bioRxiv - Immunology 2020Quote: ... IL-12 (Biolegend) or IFN-γ (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... IL-6 (Biolegend) or Tocilizumab (a kind gift from Dr Ceri Roberts in Professor Leonie Taams lab ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 (Biolegend), TNF-α (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... IL-33 (Biolegend) at 1 ng/ml final concentration ...
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bioRxiv - Immunology 2023Quote: ... IL-10 (BioLegend® Cat ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (BioLegend® Cat ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend), CD127 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were blocked using an Fc blocking antibody (Biolegend Fc-block clone: 2.4G2) and lymphocytes were stained with CD3 and CD4 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with Fc Block (BioLegend) then with fluorochrome-conjugated antibodies (BioLegend unless noted ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG Fc (Biolegend; M1310G05), anti-CD27 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocking reagent (Biolegend, 422301) was used to block Fc receptor-introduced non-specific staining ...
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bioRxiv - Microbiology 2022Quote: ... CD16/32 (Fc block-Biolegend), B220 (AF700-Biolegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptor blockade (Biolegend 101319), and anti-CD31 antibody (BD Biosciences 561073) ...
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bioRxiv - Genomics 2024Quote: ... Fc-blocking reagent (BioLegend, 422301) was used to block Fc receptor-introduced nonspecific staining ...
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bioRxiv - Immunology 2021Quote: IL-6 and IL-10 ELISAs (ELISA MAX Deluxe Set, Biolegend) were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml IL-4 + 20 ng/ml IL-13 (Biolegend) for polarization to M2 ...
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bioRxiv - Immunology 2020Quote: ... PerCP/Cy5.5 anti-mouse IL-12/IL-23 p40 (505211) (BioLegend); Purified Anti-Mouse CD16 / CD32 (2.4G2 ...
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bioRxiv - Immunology 2019Quote: ... IL-6 was evaluated using the IL-6 DIY ELISA (Biolegend). ELISA was performed as per manufacturers instructions.
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... 20ng/mL IL-7 and 20 ng/mL IL-33(BioLegend).
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bioRxiv - Biochemistry 2023Quote: ... IL-10 and IL-17A were obtained from Biolegend (California, USA); PNGase F was obtained from N-zyme Scientific (Pennsylvania ...
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bioRxiv - Immunology 2023Quote: IL-15C was formed by combining 0.75 µg IL-15 (BioLegend) with 7 µg IL-15Rα-Fc (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... The following ELISA kits were used according to manufacturer recommendations: ELISA Max human ELISA for IL-10 (Biolegend, San Diego, CA), anti-pan human IFN alpha (Interferon Source ...
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bioRxiv - Immunology 2020Quote: ... in the presence or absence of human IFN-β (500 U/ml: Pestka Biomedical Laboratories) or IL-27 (100 ng/ml: BioLegend) without adding IL-2.
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bioRxiv - Bioengineering 2021Quote: ... and IL-10 were analyzed using a human COVID-19 Cytokine Storm Panel 1 (13-plex) kit (Cat. No. 741091; BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Microbiology 2023Quote: ... Fc receptors were blocked with 0.5% anti-CD16/32 Fc block antibody (Biolegend, clone 93) diluted in PBS containing 2% goat serum (Vector Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... coverslips of each batch were incubated with 30 µg/ml anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (Cat# 409321; BioLegend, San Diego, CA) for 45 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Il-6 and IL-1β levels were quantified by ELISA (Biolegend kits).
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bioRxiv - Immunology 2022Quote: ... anti-IL-21 (3A3-N2) anti anti-IL-10Rα (3F9) from BioLegend.