IL 4 Human CHO citations for BioLegend

Quote: ... wherein each well mixed 50 μL of X2 photobleached conjugated magnetic beads with 50 μL solutions at different concentrations of a recombinant human Interleukin-8 (IL-8) protein (#574202, BioLegend, San Diego, CA, USA). The overall well concentration levels were 0.05 ...

An “alert state” ribosome population acts as a master regulator of cytokine-mediated processes

Anna Dopler, et al., bioRxiv - Immunology 2023

Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...

Identification of human tendon cell populations in healthy and diseased tissue using combined single cell transcriptomics and proteomics

AR Kendal, et al., bioRxiv - Cell Biology 2019

Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...

Combined single cell proteomics and transcriptomics reveals discrete human tendon cells populations persist in vitro and on fibrous scaffolds

Adrian Kendal, et al., bioRxiv - Cell Biology 2021

Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...

Multivalent, Bispecific αB7-H3-αCD3 Chemically Self-Assembled Nanorings Direct Potent T-cell Responses Against Medulloblastoma

Ellie A. Mews, et al., bioRxiv - Cancer Biology 2022

Quote: ... intracranial and systemic lymphocyte samples were stained with anti-human CTLA-4 antibody (Biolegend, clone BNI3, BV421). Additionally ...

Hematopoietic recovery after transplantation is primarily derived from the stochastic contribution of hematopoietic stem cells

Stefan Radtke, et al., bioRxiv - Cell Biology 2021

Quote: ... were blocked with 5ul Human TruStain FcX™ Blocking Reagent for 10 minutes at 4°C (BioLegend). 1ug of each TotalSeq™-A antibody was added to the cell suspension ...

NLRP3 inflammasome contributes to host defense against Talaromyces marneffei infection

Haiyan Ma, et al., bioRxiv - Microbiology 2020

Quote: ... cells were stained for 30 min at 4°C with FITC-conjugated anti-human CD3 antibody (Biolegend), followed by fixation and permeabilization of cells with BD Cytofix/Cytoperm™ (BD Bioscience) ...

Characterization of Neisseria gonorrhoeae colonization of macrophages under distinct polarization states and nutrients environment

María Dolores Juárez Rodríguez, et al., bioRxiv - Microbiology 2024

Quote: ... and 100 ng/ml Escherichia coli lipopolysaccharides (LPS) for 24 hrs to induce M1 polarization or 25 ng/ml IL-4 (BioLegend catalog #766202) and 25 ng/ml IL-13 (BioLegend catalog #571102 ...

Functional characterization of the biogenic amine transporter system on human macrophages

Phillip M Mackie, et al., bioRxiv - Neuroscience 2021

Quote: ... then analyzed for IL-1β using an IL-1β ELISA (BioLegend, 437004, San Diego CA) according to the manufacturer protocol.

Type 2 innate immunity regulates hair follicle homeostasis to control Demodex pathosymbionts

Roberto R. Ricardo-Gonzalez, et al., bioRxiv - Immunology 2021

Quote: ... Serum IL-22 was measured using LEGEND MAXTM Mouse IL-22 ELISA Kit (BioLegend, #436307) and analyzed using a SpectraMax M2 microplate reader using SoftMax Pro software (Molecular Devices).

P2X7 receptor signaling is not required but can enhance Th17 differentiation

Yin Yang, et al., bioRxiv - Immunology 2021

Quote: ... and supernatants were harvested for IL-6 and IL-1β ELISA (Biolegend, San Diego, CA). BMDCs were also utilized in the MLR and in vitro T cell differentiation assays.

Co-engagement of TIGIT⁺ immune cells to PD-L1⁺ tumours by a bispecific antibody potentiates T-cell response and tumour control

Xiaopei Cui, et al., bioRxiv - Immunology 2023

Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...

GDF15 mediates inflammation-associated bone loss through a brain-bone axis

Renée Van der Cruyssen, et al., bioRxiv - Immunology 2023

Quote: IL-23 levels were determined using a mouse IL-23 ELISA kit (BioLegend, Cat. 433704) following to the manufacturer’s protocol.

A novel subset of follicular helper-like MAIT cells has capacity for B cell help and antibody production in the mucosa

Owen Jensen, et al., bioRxiv - Immunology 2021

Quote: ... for 1 hour and subsequently stained overnight at 4°C with mouse anti-human TCR Vα7.2-PE (Clone 3C10, Biolegend), mouse anti-human CD161-AF488 (Clone NKR-P1A ...

Deep Proteome Profiling of Human Mammary Epithelia at Lineage and Age Resolution

Stefan Hinz, et al., bioRxiv - Cell Biology 2021

Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...

The complement pattern recognition molecule CL-11 promotes invasion and injury of respiratory epithelial cells by SARS-CoV-2

Anastasia Polycarpou, et al., bioRxiv - Immunology 2023

Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...

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