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Citations for BioLegend :
251 - 300 of 3902 citations for IL 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Supernatants were collected at 2 hours and 4 hours and analyzed using IL-6 (Biolegend). After removal of supernatant ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Bioengineering 2021Quote: ... and APC anti-human CD46 (clone TRA-2-10, Biolegend 352405). Antibody staining was performed for 20 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... APC-anti human 2B4/CD244 (2-69; BioLegend, San Diego, CA); or with the isotype control APC mouse IgG1 (MOPC-21 ...
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bioRxiv - Immunology 2019Quote: ... Antibodies include mouse anti-human ataxin-3 65042 1H9-2 (BioLegend), mouse anti-human MIC60 ab110329 (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Immunology 2022Quote: ... 1:50 diluted anti-human CD32-APC (BioLegend, clone FUN-2), and 1:50 diluted anti-human CD64-PE/DAZZLE594 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and 2 μl PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532). Additionally ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Human IL-2 ELISA MAX Deluxe kit and Human IL-12 (p70) ELISA MAX Deluxe kit in accordance with the manufacturer’s instructions (BioLegend).
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... IL-4-PE Dazzle594 and IL-13-BV421 (Biolegend). Sample acquisition was performed on a LSR Fortessa (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... The His tagged ACE2 variants were detected using anti-His mAb (Cat. No. 652505, BioLegend).
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with intracellular monoclonal antibody-fluorochrome conjugates: IL-2-PE (BioLegend, cat.# 500307), TNF-α-FITC (BD ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822] ...
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Immunology 2020Quote: ... or monoclonal anti-mouse IL-4Rα (mα-IL-4Rα) (Biolegend). In each group ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... and 20ng/mL of IL-15 and IL-18(Biolegend).
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bioRxiv - Immunology 2023Quote: ... IL-4 (11B11) and IL-5 (TRFK5) were from Biolegend. Following surface and/or intracellular stain ...
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD206 APC (1:50, clone 15-2, BioLegend, cat # 321110), Anti-human CD4 Alexa700 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... PE-anti-human-Nectin-2 (TX31) (all from BioLegend, San Diego, CA). The following antibodies were used for in vivo blocking experiments ...
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bioRxiv - Immunology 2022Quote: ... 1-2×106 cells were incubated with Human TruStain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406 ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2021Quote: ... IL-33 (Biolegend) or 20 μg of NMU (US Biological ...
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bioRxiv - Bioengineering 2020Quote: ... IL-6 (Biolegend) and TGF-β (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... IL-22 (Biolegend), mouse TGFβ1 (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: ... IL-5 (BioLegend) and IL-13 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... IL-17A (BioLegend). For the detection of intracellular IL-17A expression ...
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bioRxiv - Immunology 2021Quote: ... IL-17 (Biolegend) and CCL17 (R&D Systems) ...
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bioRxiv - Immunology 2020Quote: ... IL-12 (Biolegend) or IFN-γ (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... IL-6 (Biolegend) or Tocilizumab (a kind gift from Dr Ceri Roberts in Professor Leonie Taams lab ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 (Biolegend), TNF-α (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... IL-10 (BioLegend® Cat ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (BioLegend® Cat ...
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bioRxiv - Microbiology 2023Quote: ... IL-33 (Biolegend) at 1 ng/ml final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Immunology 2023Quote: ... aliquots of conditioned media were collected for measuring IL-2 and IFNγ by ELISA (ELISA MAX, BioLegend). Cells were washed and stained with anti-mouse CD3ε-FITC (145-2C11 ...
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bioRxiv - Immunology 2021Quote: IL-6 and IL-10 ELISAs (ELISA MAX Deluxe Set, Biolegend) were performed according to the manufacturer’s instructions.