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601 - 650 of 3309 citations for IL 13 Human CHO since 2019

• The asthma gut microbiota influences lung inflammation in gnotobiotic mice

  Naomi G. Wilson, et al., bioRxiv - Microbiology 2022

  Quote: ... PE anti-mouse IL-17A (Clone TC11-18H0.1, Biolegend), PerCP-Cy™5.5 anti-mouse TCR β chain (Clone H57-597 ...
• Commensal-specific CD4 T cells promote inflammation in the central nervous system via molecular mimicry

  Zachary White, et al., bioRxiv - Immunology 2022

  Quote: ... anti-IL-17A-AF700 (BioLegend 506914: Clone: TC11-18H10.1), anti-IL-22-APC (Invirtogen 17-7222-80 ...
• Hyperthermia elevates brain temperature and improves behavioural signs in animal models of Autism spectrum disorder

  Ana Belen Lopez-Rodriguez, et al., bioRxiv - Neuroscience 2022

  Quote: ... ELISA MAX Mouse IL-1β (Biolegend, San Diego, US), and DuoSet Mouse TNF-α (R&D Systems ...
• B cell receptor induced IL-10 production from neonatal CD19+CD43- cells depends on STAT5 mediated IL-6 secretion

  Jiro Sakai, et al., bioRxiv - Immunology 2022

  Quote: ... ELISA MAX™ Standard Set Mouse IL-10 (BioLegend), Mouse TNF-α DuoSet ELISA (R&D Systems) ...
• The CARD8 inflammasome drives CD4⁺ T-cell depletion in HIV-1 infection

  Qiankun Wang, Liang Shan, bioRxiv - Microbiology 2023

  Quote: ... Cytokines used in this study: IL-2 (BioLegend #589106), IL-7 (BioLegend #581904) ...
• Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T cell differentiation

  Mingeum Jeong, et al., bioRxiv - Immunology 2022

  Quote: ... and 10 ng/ml IL-23 (both from Biolegend); for Treg - 1 µg/ml anti-IFN-γ (clone R4-6A2) ...
• Integration of Avidity and Differentiation is enabled by CD8⁺ T-cell sensing of IFN-γ

  Lion F.K. Uhl, et al., bioRxiv - Immunology 2023

  Quote: ... which included different combinations of IL-12 (Biolegend, #577004), IL-18 (Biolegend ...
• Cellular iron governs the host response to malaria

  Sarah K. Wideman, et al., bioRxiv - Immunology 2023

  Quote: ... 5 μg/mL anti-mouse IL-4 (504102, BioLegend), 10 ng/mL IL-12 (505201 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... IL-2 (1:100 dilution; clone JES6-5H4, BioLegend) and anti-IL-4 (1:100 dilution ...
• The receptor binding domain of SARS-CoV-2 spike protein fused with the type IIb E. coli heat-labile enterotoxin A subunit as an intranasal booster after mRNA vaccination

  He-Chin Hsieh, et al., bioRxiv - Microbiology 2023

  Quote: ... and IL-17A using a commercial ELISA kit (Biolegend). The ELISA kit measured the concentration of these cytokines by utilizing specific antibodies that bind to the cytokines in the sample ...
• Membrane-bound Interleukin-1α mediates leukocyte adhesion during atherogenesis

  Christina Mäder, et al., bioRxiv - Immunology 2023

  Quote: ... Anti-biotin IL-1α antibody (1µg/ml, Biolegend, USA) or rat IgG isotype control (1 µg/ml ...
• Engineered probiotics limit CNS autoimmunity by stabilizing HIF-1α in dendritic cells

  Liliana M. Sanmarco, et al., bioRxiv - Immunology 2023

  Quote: ... PE anti-mouse IL-17A (506904, BioLegend, 1:100). Cells were acquired on a Symphony A5 (BD Biosciences ...
• Spermidine/spermine N1-acetyltransferase controls tissue-specific regulatory T cell function in chronic inflammation

  Teresa Neuwirth, et al., bioRxiv - Immunology 2024

  Quote: ... anti-IL-2 PE (Biolegend, Cat: 340450, 1:50), anti-IL-17A APC (eBioscience ...
• Quantitative effects of co-culture on T cell motility and cancer-T cell interactions

  Xinyue Li, et al., bioRxiv - Systems Biology 2024

  Quote: ... and 30 U/ml mouse recombinant IL-2 (Biolegend).
• The dysregulated IL-23/T_H17 axis in endometriosis pathophysiology

  Danielle J. Sisnett, et al., bioRxiv - Immunology 2023

  Quote: ... and 500ng/mL anti-IL-4 (500837, BioLegend, USA) purchased from R&D Systems unless stated otherwise ...
• STK25 is an IRF5 kinase that promotes TLR7/8-mediated inflammation

  Matthew R. Rice, et al., bioRxiv - Biochemistry 2023

  Quote: IL-6-APC (BioLegend, 504508, MP5-20F3, 1 μL)
• Development of a generalisable tryptophan-optimised quenchbody biosensor based on a synthetic nanobody library

  Jordan H. Cater, et al., bioRxiv - Bioengineering 2024

  Quote: ... Magnetic beads displaying recombinant IL-6 protein (BioLegend #570806) were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203) ...
• FAS^lpr gene dosage tunes the extent of lymphoproliferation and T cell differentiation in lupus

  Ritu Bohat, et al., bioRxiv - Immunology 2023

  Quote: ... anti-IL-4-PE (BioLegend Cat# 504102 (also 504101), RRID:AB_315316) ...
• Conversion of natural cytokine receptors into orthogonal synthetic biosensors

  Hailey I. Edelstein, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... 100 or 250 ng/mL IL-10 (Biolegend #573206), or 250 ng/mL TNF (ACROBiosystems ...
• Transcriptional responses of brain endothelium to Plasmodium falciparum patient-derived isolates in vitro

  Caroline Askonas, et al., bioRxiv - Cell Biology 2024

  Quote: ... or 1 ng/mL IL-1 beta (579402, BioLegend) and compared to EGM2min medium ...
• The complement regulator CD55 modulates TLR9 signaling and supports survival in marginal zone B cells

  Iris Lee, et al., bioRxiv - Immunology 2024

  Quote: ... and cytokine stains with IL-6 (MP5-20F3; Biolegend), IL-10 (JES5-16E3 ...
• Commensal Bacteria Maintain a Qa-1^b-restricted Unconventional CD8⁺ T Population in Gut Epithelium

  Jian Guan, et al., bioRxiv - Immunology 2023

  Quote: ... or a combination of 100ng/ml IL-7 (BioLegend) and 100ng/ml IL-18 (BioLegend ...
• Triacylglycerol synthesis enhances macrophage inflammatory function

  Angela Castoldi, et al., bioRxiv - Immunology 2020

  Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
• Disruption of the CSF-1-CSF-1R axis alters cerebellar microglia and is associated with motor and social interaction defects

  Veronika Kana, et al., bioRxiv - Neuroscience 2019

  Quote: ... Supernatants containing 70 µg of total protein were used to determine IL-34 concentration using an IL-34 ELISA kit (BioLegend, San Diego, CA).
• SHORT-TERM CALORIC RESTRICTION IN MICE PROMOTES RESOLUTION OF ATHEROSCLEROSIS, WHILE WEIGHT REGAIN ACCELERATES ITS PROGRESSION

  Bianca Scolaro, et al., bioRxiv - Immunology 2023

  Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
• Engineering CAR-NK cells to secrete IL15 sustains their anti-AML functionality, but is associated with systemic toxicities

  Ilias Christodoulou, et al., bioRxiv - Immunology 2021

  Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
• Proliferating CLL cells express high levels of CXCR4 and CD5

  Daniel Friedman, et al., bioRxiv - Cancer Biology 2023

  Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
• mSOD1-NSG mice: a new in vivo model to test human T cells in ALS

  David J Graber, et al., bioRxiv - Immunology 2021

  Quote: ... human CD4-APC (Biolegend), human FoxP3-APC (eBioscience) ...
• Midbrain organoids with an SNCA gene triplication model key features of synucleinopathy

  Nguyen-Vi Mohamed, et al., bioRxiv - Neuroscience 2021

  Quote: ... Human TruStain FcXTM (Biolegend). Protected from light ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD3 (BioLegend, #317324), mouse CD45 (BioLegend #103130) ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD45 (BioLegend #304014), human CD25 (BioLegend #302606) ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human FOXP3 (BioLegend #320116), Alexa Fluor® 647-conjugated anti-mouse integrin αvβ5 Ab (clone ALULA ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD45 (BioLegend #304014), Zombie NIR™ Fixable Viability Kit (BioLegend #423106 ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD25 (BioLegend #302606), human FOXP3 (BioLegend #320116) ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD8 (BioLegend #344712), human CD45 (BioLegend #304014) ...
• Legionella pneumophila inhibits type I interferon signaling to avoid cell-intrinsic host cell defense.

  Charles N.S. Allen, et al., bioRxiv - Microbiology 2023

  Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
• Legionella pneumophila inhibits type I interferon signaling to avoid cell-intrinsic host cell defense.

  Charles N.S. Allen, et al., bioRxiv - Microbiology 2023

  Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
• Engineering multivalent Fc display for FcγR blockade

  Ekaterina Petrova, et al., bioRxiv - Bioengineering 2024

  Quote: ... Recombinant human FcγIIa (Biolegend), FcγRIIb (Biolegend) ...
• Single-cell transcriptional landscape of temporal neutrophil response to burn wound in larval zebrafish

  Yiran Hou, et al., bioRxiv - Immunology 2024

  Quote: ... Human TruStain FcX (BioLegend) was added at 1:20 to block Fc receptors for 10 minutes ...
• CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease

  Sujit Silas Armstrong, et al., bioRxiv - Immunology 2024

  Quote: ... human TGFβ1 (580706; BioLegend), human IL-12 (219-IL;R&D Sys-tems) ...
• In silico design of Phl p 6 variants with altered folding stability significantly impacts antigen processing, immunogenicity and immune polarization

  Petra Winter, et al., bioRxiv - Immunology 2020

  Quote: ... Cytokine levels in supernatants were analyzed using the LEGENDplex™ Mouse Th Cytokine Panel (13-plex, BioLegend) according to the manufacturer’s instructions.
• Oral supplementation of gut microbial metabolite indole-3-acetate alleviates diet-induced steatosis and inflammation in mice

  Yufang Ding, et al., bioRxiv - Cell Biology 2023

  Quote: ... A panel of 13 cytokines was quantified with a bead-based ELISA kit (BioLegend, San Diego, CA) following the manufacturer’s protocol ...
• Galectin-anchored indoleamine 2,3-dioxygenase suppresses local inflammation

  Evelyn Bracho-Sanchez, et al., bioRxiv - Bioengineering 2021

  Quote: ... IL-6 was quantified using ELISA developed in the laboratory using biotin anti-rat IL-6 antibody (cat. # 517703) and purified (coating) anti-rat IL-6 antibody (cat. # 517701, Biolegend, San Diego, CA, USA). Coating antibody was diluted in 100 mM NaHCO3 ...
• Innate immune response to SARS-CoV-2 infection contributes to neuronal damage in human iPSC-derived peripheral neurons

  Vania Passos, et al., bioRxiv - Microbiology 2022

  Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
• LXR alters CD4⁺ T cell function through direct regulation of glycosphingolipid synthesis

  Kirsty E Waddington, et al., bioRxiv - Immunology 2020

  Quote: ... IL-4-APC (clone MP4-25D2, BioLegend Cat# 500812, RRID:AB_315131), IL-17A-AlexaFluor488 (clone eBio64Dec17 ...
• Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cells

  Dingxi Zhou, et al., bioRxiv - Immunology 2021

  Quote: ... IL-7Rα (135002, BioLegend, manually conjugated with AF488, 1:100), IL-2Rα (154202 ...
• Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cells

  Dingxi Zhou, et al., bioRxiv - Immunology 2021

  Quote: ... IL-2Rα (154202, BioLegend, manually conjugated with AF568, 1:100), γc (132307 ...
• Functionally overlapping variants control TB susceptibility in Collaborative Cross mice

  Clare M Smith, et al., bioRxiv - Microbiology 2019

  Quote: ... IL-10 (JES5-16E3) and CD107a-PE (1D4B) from Biolegend and IL-4-Alexa Fluor 488 (11B11 ...
• Pregnancy alters innate immune responses to Zika virus infection in the genital tract

  Kelsey E. Lesteberg, et al., bioRxiv - Immunology 2019

  Quote: ... and anti-mouse IL-10 APC (clone JES5-16E3, Biolegend). Ghost Violet 510 dye (Tonbo Biosciences ...
• A combined microphysiological-computational omics approach in dietary protein evaluation

  Paulus G.M. Jochems, et al., bioRxiv - Bioengineering 2020

  Quote: ... supernatant was collected and IL-8 (BioLegend, London, United Kingdom), IL-6 (Biolegend ...