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Citations for BioLegend :
201 - 250 of 4172 citations for IFN gamma Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... anti-CD8 (clone 53-6.7) and anti-IFN-γ (clone XMG1.2) were from BioLegend. Anti-CD45 (clone 30-F11) ...
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bioRxiv - Immunology 2020Quote: ... before staining with IFN-γ (eBioscience, 12-73111-81) and IL-17 (BioLegend, 506904). Data were collected using a BD LSR Fortessa flow cytometer and analyzed using FlowJo Software (Tree Star ...
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bioRxiv - Immunology 2022Quote: ... successfully transduced cells were collected after 10 min incubation with human IFN-α (BioLegend), IFN-γ (BioLegend) ...
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bioRxiv - Microbiology 2023Quote: ... intracellular cytokine staining was performed with antibodies to IFN-γ (PE, clone XMG1.2; BioLegend) and TNF-α (APC ...
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bioRxiv - Immunology 2023Quote: ... The IFN-γ level in supernatants was quantitated using an ELISA kit (BioLegend, US) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Immunology 2024Quote: IL-2 and IFN-γ levels were measured with a sandwich ELISA kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 Brilliant Violet 421 and anti-IFN-γ PE (Biolegend. San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... followed by addition of relevant detection mAbs: biotin anti-human IFN-γ (Clone: 4S.B3, BioLegend); biotin anti-human TNF-α (Clone ...
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bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
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bioRxiv - Immunology 2021Quote: ... Th2 differentiation was achieved using 1 μg/mL anti-IFN-γ antibodies (clone: XMG1.2, Biolegend), 5 ng/mL IL-2(PeproTech) ...
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bioRxiv - Microbiology 2023Quote: ... Intracellular staining antibodies: anti-IFN-γ conjugated to Brilliant Violet 510 (1:200, Biolegend, USA), anti-IL-17A conjugated to Brilliant Violet 421 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... and cytokines (IFN-y and IL-5) were measured by ELISA (Biolegend. San Diego, CA). For intracellular cytokine determination ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with anti-IFN-γ (XMG1.2) and anti-TNF-α (MP6-XT22) from BioLegend. For intranuclear Foxp3 staining ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ and IL-4 production were determined using ELISA kits (BioLegend, San Diego, CA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... cells were permeabilized and labeled with the described cocktail plus APC-IFN-γ (BioLegend Cat# 505810) and PE-IL-17A (BioLegend Cat# 506903) ...
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bioRxiv - Immunology 2022Quote: ... Pelleted cells were resuspended in BD Perm/Wash™ Buffer containing anti-IFN-γ antibodies (Biolegend) for 30 min and analyzed by CytoFLEX flow cytometer.
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bioRxiv - Synthetic Biology 2020Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFN◻ by ELISA (BioLegend). All assays were performed in triplicate and average IFN◻ production was plotted with standard deviation.
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bioRxiv - Microbiology 2020Quote: ... cells were stained with Alexa Fluor® 647-conjugated anti-human IFN-γ antibody (502516, Biolegend) and PE-conjugated anti-human IL-17A antibody (512305 ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... and then stained intracellularly with APC-conjugated anti-IFN-γ (1:100, Biolegend Cat# 507810, RRID:AB_756180), PE-conjugated anti-IL-17 (1:30 ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and IFN-γ from human PBMCs were measured by LEGENDplex™ Human Inflammation Panel (Biolegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The abundance of Nectin-2+ monocytes and NK cell intracellular cytokine production of IFN-γ (4S.B3, Biolegend) were analyzed by flow cytometry (BD LSRFortessa™ Cell Analyzer ...
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bioRxiv - Immunology 2020Quote: ... cytokines (hIL-10 20ug, mIL-15 15ug bid, IFN-γ 5×104 IU from BioLegend Inc., CA) were injected intraperitoneally in mice on day 1 ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Cancer Biology 2022Quote: ... and supernatants were collected and evaluated for IFN-β secretion by ELISA (LEGEND MAX mIFN-β ELISA, Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IL-1ß and IFN-γ were measured using bead-based multiplex assays (740180; 740589; 740929; LEGENDplex™ BioLegend). Serum samples were screened for CMV-specific IgG antibodies with the CMV-IgG-ELISA PKS Medac enzyme immunoassay (115-Q-PKS ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained for surface markers followed by intracellular cytokines using antibodies specific for IFN-y (4S.B3, 1:40) (Biolegend) IL-2 (MQ1-17H12 ...
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bioRxiv - Immunology 2022Quote: ... and stained with a cocktail of fluorescently labeled anti-cytokine antibodies: IFN-γ PE/Dazzle 594 (Biolegend, cat.505845), TNF-α PE/Cyanine7 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... and stained for intracellular cytokines (IFN-γ PE #505808, IL-4 BV421 #504120, IL-17A PE-Cy7 #506922, Biolegend) with the Cyto-Fast™ Fix/Perm Buffer Set (#426803 ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were obtained and IFN-γ and TNF-α concentrations were quantified using ELISA MAX Standard Sets (Biolegend; cat.# 430801 for IFN-γ ...
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bioRxiv - Cancer Biology 2024Quote: ... IFN-γ secretion to validate CAR-T cell activation was measured using the Human INF-γ ELISA kit (Biolegend) or Human IFN-γ DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for 50 minutes with anti-mouse IL-17A APC (TC11-18H10.1, 506916), IFN-γ BV421 (XMG1.2, 505830) and TNF-ɑ FITC (MP6-XT22, 506 304) all from Biolegend. Fc block (anti-CD16/CD32 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... fixed with 2% formaldehyde and permealized with BD Perm/Wash buffer (Fischer) and stained with anti IFN-γ (clone XMG1.2; BioLegend) and anti-GM-CSF (clone MP1-22E9 ...
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-TNF-PE (MAb1) and anti-IFN-γ-APC (4S.B3) from BioLegend at room temperature for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... Cat# 137024), granzyme B (QA18A28, Cat# 396413), TNF (MP6-XT22, Cat# 506329), IFN-γ (XMG1.2, Cat# 505846) were obtained from BioLegend. Anti-Foxp3 (FJK-16s ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized using BD Fix/Perm for 20 min at RT and stained intracellularly with anti-IFN-γ (1:100 dilution; clone XMG1.2, Biolegend), anti-TNF-α (1:100 dilution ...
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bioRxiv - Immunology 2022Quote: ... 50 μl of supernatants from cultures of T cell clones were collected after 48 hrs of stimulation and added to each well of 96-well round-bottom plates precoated with IFN-γ (clone MD-1) capturing antibodies (BioLegend). After overnight incubation ...
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bioRxiv - Cell Biology 2021Quote: ... CD59 (PE, 143103), PD-1 (BV605, 135220), CD44 (BV711, 103057), CD69 (PE-Cy7, 104512), IFN-γ (APC, 505810) (all from Biolegend). The Foxp3 Fix/Perm Kit was used for intracellular staining (421403 ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IFN-γ neutralizing antibody (XMG1.2, BioLegend, 10ug/mL) and rmIL-4 (R&D ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... in the presence or absence of human IFN-β (500 U/ml: Pestka Biomedical Laboratories) or IL-27 (100 ng/ml: BioLegend) without adding IL-2.