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Citations for BioLegend :
451 - 500 of 706 citations for ICOS Rhesus Macaque HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by Fc receptor blockade for 10 min and then incubation in permeabilization buffer (BioLegend, 421002) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... samples were incubated with Fc blocking solution (1:100 anti-mouse CD16/32, Biolegend Catalogue # 101302) in FACS buffer (1x PBS with 2% fetal bovine serum) ...
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bioRxiv - Immunology 2023Quote: Zombie Aqua Fixable Viability Dye and Human TruStain FcX Fc Receptor Blocking Solution were from BioLegend. CF700-SE and CF750-SE were bought from Biotium ...
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bioRxiv - Cancer Biology 2023Quote: ... human and mouse FC receptors were blocked using Human TruStain FcX (BioLegend, San Diego, CA, USA) and purified anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc receptor blocking was performed using TruStain FcX PLUS anti-mouse CD16/32 Antibody (BioLegend, 156604) prior to staining with fluorophore-conjugated antibodies at the concentrations recommended by the manufacturers ...
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bioRxiv - Neuroscience 2023Quote: ... the pellets were resuspended in 90 µL FACS buffer with Fc block (4G2, 1:90, BioLegend) and transferred to a 96 well-plate ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc blocked (TruStain FcX™, Cat. 101320, and True-Stain Monocyte Blocker™ Cat. 426102 Biolegend) and stained with conjugated primary antibodies as per manufacturer’s protocol and pre-titrated antibody dilutions (supplementary table S1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cell suspensions were treated with purified anti-CD16/32 (Fc receptor block, clone 93; BioLegend), and then stained with fluorochrome-conjugated monoclonal antibodies ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked using TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody from Biolegend. Surface staining was carried out by incubating cells with staining antibodies in FACS buffer (PBS with 2% FBS and 1 mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... then anti CD16 /32 were added for Fc- receptors blocking on ice for 10 minutes (Biolegend, Purified anti-mouse CD16/32 AntibodyRat IgG2a ...
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bioRxiv - Immunology 2024Quote: DAPI (#11034-56, Nacalai Tescue) and Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences) were added to the cells just before staining with specific antibodies against cell surface molecules ...
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bioRxiv - Immunology 2024Quote: ... human cells were blocked with Human TruStain FcX Fc Receptor Blocking Solution (Biolegend, #422302, 1:100) in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend ...
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bioRxiv - Molecular Biology 2024Quote: ... one million cells were first resuspended in100LμL buffer containing 5LμL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15Lminutes ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in MACS buffer and incubated with PE–anti-human FC antibody (Biolegend, M1310G05) in MACS buffer for 30 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 420 x g for 5 minutes and resuspended in anti-CD16/CD32 (Fc Block; Biolegend) diluted 1:50 in FACS buffer (PBS with 2% BSA and 1 mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... The nonspecific binding of Fc receptors was blocked using anti-CD16/32 antibody (BioLegend, catalog no. 101302) on ice for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend), and Brilliant Stain Buffer (BD Bioscience) ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were stained with Zombie-NIR followed by Fc block with anti-mouse CD16/32 antibody (Biolegend). Cells were then stained for cell surface markers as described above ...
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bioRxiv - Cell Biology 2021Quote: ... they were blocked in 1% BSA in PBS and Fc blocker (unlabeled anti-CD16/32, Biolegend 101302) for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7 ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... The following proteins and concentrations were used: ICAM-1-Fc (Cat. 553006, BioLegend, at 10 μg/mL), CD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2019Quote: ... Fc-Block was performed (10µg/ml purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse), after which cells were stained with Fixable Viability Staining e780 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with Fc block TruStain FcX (CD16/32) to block nonspecific FcR binding (#422302, Biolegend). To detect MHC-I and MHC-II ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... Fc receptors were then blocked with an anti-CD16/CD32 antibody (BioLegend, San Diego, CA, U.S.A., #553141). Cells were washed and then incubated with conjugated antibodies (FITC-CX3CR1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in washing buffer (PBS 0.04% BSA) and incubated with mouse Fc blockers (FcX, Biolegend). Each sample was then incubated with either TotalSeq anti-mouse Hashtag Antibody 1 (Biolegend #155861 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% NaN3 in 1X PBS) and blocked with Fc receptor antibody specific for FcyR III/II (Biolegend TruStain FcX PLUS anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... Nonspecific binding was blocked by incubating cells anti-mouse CD16/32 Fc block (Biolegend; cat. no. 101310). Sytox-Green viability dye (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in semi-facs buffer in the presence of Fc receptor blocking antibody (BioLegend, 101302) and stained with antibodies at a 1:200 dilution with the myeloid or lymphoid antibody cocktails ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Immunology 2023Quote: ... the cells were blocked in 1% BSA in PBS and Fc blocker (anti-CD16/32, Biolegend 101302) for 10 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cells that were used in CITE-seq experiments were blocked with TruStain Fc block (BioLegend; Cat. 422301) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... CAR-M cells was prepared and the Fc receptor was blocked with anti-moCD16/32 antibody (BioLegend) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Immunology 2022Quote: ... cells were pre-treated with 2.5 μg/mL anti-CD16/32 (Fc Block; 2.4G2; BioLegend, San Diego, CA) for 15 minutes before continuing with surface staining ...
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bioRxiv - Immunology 2021Quote: ... 1×105-1×106 cells were stained after Fc-blocking with TruStain FcX (BioLegend, San Diego, CA, USA) and incubation with Zombie Aqua fixable viability dye (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were aspirated and cells were then treated with Fc block (clone 93, Ref: 101302, BioLegend, 1:1000) at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...