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Citations for BioLegend :
651 - 700 of 4726 citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Microbiology 2022Quote: Cytokine concentration of the basolateral compartments were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... IFN-γ secretion to validate CAR-T cell activation was measured using the Human INF-γ ELISA kit (Biolegend) or Human IFN-γ DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... endogenous biotin and avidin proteins were successively blocked using an Avidin/Biotin Blocking Kit (Biolegend), for 10 min each ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilisation for intracellular staining was performed with IC perm buffer or FoxP3 Buffer set (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were stained by using True-Nuclear transcription factor buffer set (Biolegend, San Diego, CA). Briefly ...
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bioRxiv - Genomics 2022Quote: ... at room temperature for 30 minutes using True-Nuclear Transcription Factor Buffer Set (Biolegend #424401) to assess GATA3 protein level ...
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bioRxiv - Immunology 2021Quote: ... T-bet and Ki67 was performed using True-Nuclear™ Transcription Factor Buffer Set (BioLegend), according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed and permeabilized using Cyto-Fast Fix/Perm Buffer Set (#426803, BioLegend, USA), prior to staining for 20 min at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... were used to detect IgG1 antibodies upon addition of TMB substrate (TMB substrate set, Biolegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... Staining of intracellular BCL2 was performed with True-Nuclear™ Transcription Factor Buffer Set (Biolegend) as per manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend) before intracellular staining of Arginase I ...
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bioRxiv - Microbiology 2023Quote: ... the cells were stained using True-Nuclear™ Transcription Factor Buffer Set (Cat: 424401; Biolegend) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2024Quote: IL-2 and IFN-γ levels were measured with a sandwich ELISA kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... antibody or 100µL of PBS containing 0.2µg TruStain FcX™ and 0.04µL Zombie AquaTM Fixable Viability Kit (BioLegend cat#: 423102) and incubated at room temperature (RT ...
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bioRxiv - Cell Biology 2021Quote: ... BSA/0.1% (v/v) TritonX-100 in PBS for 30 min at RT and stained for actin with Alexa594-conjugated phalloidin (BioLegend, USA) diluted in 0.3% (w/v ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Microbiology 2021Quote: THP-1 human macrophages were treated with mouse anti-human Fc block (Ref: 422302, BioLegend), for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... which consisted of anti-human Alexa Fluor® 700 anti-human CD3 (BioLegend, cat. 300324), Brilliant Violet 785™ anti-human CD4 (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Th1 cell released cytokines were analyzed by LEGENDplexTM Human Th1 Panel (Biolegend, Cat# 741035). Five human T cell cytokines including IL-2 ...
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bioRxiv - Pathology 2022Quote: ... human and murine Fc receptors were blocked using Human TruStain FcX (Biolegend, #422302, 1/20) and anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
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bioRxiv - Immunology 2024Quote: ... Human cells were blocked by Human TruStain FcX Fc receptor blocking solution (#422301 from BioLegend). Flow cytometry analysis was performed using an AccuriC6 Plus platform ...
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bioRxiv - Immunology 2021Quote: ... The coronavirus protein panel consisted of the following flurorescent probes: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), WIV1 RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and proceeded to Annexin V-fluorescein isothiocyanate (FITC)/PI staining according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI; BioLegend, San Diego, CA, USA). Cells stained with Annexin V (both Annexin V+- and Annexin V+/PI+-cells ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Immunology 2019Quote: ... The presence of human GM-CSF in the supernatant of eMSCs co-cultures with CD34+ HSPCs was assessed by enzyme-linked immunosorbent assay (ELISA) using the human GM-CSF ELISA MAX kit (Biolegend) as per manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T lymphocytes were purified (> 98%) by negative selection with MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3 ...
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytokines in frozen cell culture supernatant or frozen plasma were analyzed with a customized multiplex human or mouse cytokine Legendplex kit (BioLegend). Samples were measured at the MACS Quant Analyzer10 (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: NK cells were isolated from frozen PBMCs by negative selection using the MojoSort™ Human NK Cell Isolation Kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were collected from effector-target cell co-cultures and analyzed for IFNy using either ELISA MAX Deluxe Human IFNγ kit (BioLegend) or Human IFNγ DuoSet ELISA (R&D Systems ...
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bioRxiv - Immunology 2023Quote: Cytokines secreted in the culture supernatant of cytokine-stimulated sip-T were measured by a LEGENDplex Human CD8/NK Panel kit (BioLegend) following manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... permeabilised and stained for transcription factors using the True-Nuclear Transcription Factor Buffer Set (Biolegend, #424401). Cytokines and transcription factors in the same FACS panel were stained with the FOXP3 transcription factor staining kit (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... IL-1α and IL-6 were quantified with Mouse IFN-γ ELISA MAXTM Deluxe Set (Biolegend), Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend ...