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Citations for BioLegend :
301 - 350 of 5396 citations for Guanine Nucleotide Binding Protein G Protein Alpha Activating Activity Polypeptide O GNAO1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Protein levels in plasma and skin tissue protein extracts were measured using defined analyte panels: Legendplex Mouse Inflammation Panel and Inflammatory Chemokine Panel (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Nucleocapsid level on exosomes was measured by ELISA using precoated ELISA plates (Legend Max SARS-CoV2 Nucleocapsid protein ELISA Kit, 448007, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Immunology 2020Quote: ... 5min) and incubated in 100 μl of binding buffer containing 0.125 μg of streptavindin-APC (Biolegend) for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with annexin V binding buffer containing FITC annexin V (1:200) (Biolegend), propidium iodide (PI ...
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bioRxiv - Microbiology 2022Quote: ... IFN-g-PE-Cy7 (4S.B3 clone, Biolegend), IL-2-APC (MQ1-17H12 clone ...
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bioRxiv - Microbiology 2023Quote: ... IFN-g-PE-Cy7 (4S.B3 clone, Biolegend), IL-2-APC (MQ1-17H12 clone ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Organoids were cultivated in growth medium as previously described (61) with minor changes (50% mouse Wnt3a-conditioned medium, 50ng/mL mouse Wnt3a recombinant protein (Biolegend, USA), 15% mouse R-spondin1-conditioned medium ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2020Quote: ... YU2-gp140 fold-on, and hepatitis B surface antigen (HBs Ag) (Protein Specialists, hbs-875) conjugated to streptavidin Alexa Fluor 647 (Biolegend, 405237), streptavidin PE (BD biosciences ...
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bioRxiv - Microbiology 2022Quote: The level of TNF-α protein expression was evaluated after cell transduction with TPA-TNFα in the cultured medium using human TNFα ELISA kit (Biolegend, UK). First ...
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bioRxiv - Neuroscience 2022Quote: Global protein concentration of the BV2 cells post treatment was assessed via flow cytometry using intracellular protein staining using the True-Nuclear Transcription Factor Buffer Set (Biolegend # 424401). Cells were incubated with primary antibodies for 30 minutes - 1:100 Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Immunology 2023Quote: To determine galectin-3 or galectin-8 expression the following: intracellular protein and lysosomes were stained using anti-Lamp1-BV421 (clone H4A3, BioLegend, USA), anti-Galectin-3-AF488 (clone B2C10 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... then washed with AnnexinV binding buffer and incubated with APC Annexin V as per manufacturer’s instructions (BioLegend). For phospho-flow cytometric staining ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in binding buffer containing Alexafluor647-Annexin (Biolegend; 1:200 in binding buffer ...
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bioRxiv - Microbiology 2022Quote: ... C3b binding was detected with PE-conjugated mouse monoclonal anti-C3b/iC3b (5 μg/mL, BioLegend #846103) and C5b-9 membrane deposition/insertion was detected with mouse monoclonal anti-C5b-9 antibody (8 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with Fc block TruStain FcX (CD16/32) to block nonspecific FcR binding (#422302, Biolegend). To detect MHC-I and MHC-II ...
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bioRxiv - Neuroscience 2022Quote: ... binding the epitope 103-108 still present in C-terminal truncated forms of α-synuclein (Biolegend, Inc.). In brief ...
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bioRxiv - Immunology 2023Quote: ... Nonspecific binding was blocked by incubating cells anti-mouse CD16/32 Fc block (Biolegend; cat. no. 101310). Sytox-Green viability dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... or restimulated on day 11 in 96-well plates with 2.5ug/ml recombinant SARS-CoV-S protein S1 (Biolegend 792906, carrier-free) for 8h in the presence of 1ug/ml Golgiplug and 0.7ug/ml Golgistop ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2021Quote: ... Cells with successful PD-L1 deletion were isolated by two rounds of FACS sorting after O/N stimulation with 100 ng/ml IFNγ and staining with PD-L1-PE/Cy7 (clone 10F.9G2, Biolegend 124314, 1:200). Cells retaining PD-L1 expression were isolated simultaneously and served as controls.
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bioRxiv - Biochemistry 2022Quote: ... Next steps of ELISA assay were identical to the ones described with sole exception that instead of o-phenylenediamine dihydrochloride as a peroxidase substrate in these experiments was used TMB high sensitivity substrate solution (BioLegend, San Diego, CA) and absorbance was recorded at 450 nm.
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bioRxiv - Immunology 2021Quote: ... Gr1(Ly6C/G)-PerCP-Cy5.5 (BioLegend, no. 108427), and CD45-APC (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... and 5ng/ml recombinant human G-CSF (BioLegend). The cells were then incubated for 16 hours at 37°C and 5%CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... were prepared in 1× Annexin V Binding Buffer (100 mM HEPES, 25 mM CaCl2, 1.4 M NaCl; BioLegend). To analyze cells ...
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bioRxiv - Neuroscience 2020Quote: 250 μg protein from each human brain sample lysate was used for immunoprecipitation (IP) with a combination of Tau12 (Biolegend, cat. no. 806501), Tau 5 (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants containing 70 µg of total protein were used to determine IL-34 concentration using an IL-34 ELISA kit (BioLegend, San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
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bioRxiv - Immunology 2024Quote: ... Surface proteins were then stained at 4°C for 30 minutes using the following markers: NIR Zombie dye APC-Cy7 (Biolegend, Cat No. 423105), CD20 PE (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... interferon-g at 2.5ng/mL (wt/vol, IFNg, BioLegend) and dexamethasone 1mM/mL (wt/vol ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... wherein each well mixed 50 μL of X2 photobleached conjugated magnetic beads with 50 μL solutions at different concentrations of a recombinant human Interleukin-8 (IL-8) protein (#574202, BioLegend, San Diego, CA, USA). The overall well concentration levels were 0.05 ...
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bioRxiv - Neuroscience 2023Quote: ... TruSeq anti-Nuclear Pore Complex Proteins Hashtags were applied to track the brain region of origin for each cell nuclei (BioLegend, CAT: 682205, 682207, 682209). We counterstained nuclei with a far-red DNA fluorescent intercalator ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific bindings were blocked by addition of 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) and incubation for 10 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... washed once in PBS containing 0.9 mM Ca2+ and 0.5 mM Mg2+ and then stained with AnnV-FITC for 15 minutes in Annexin binding buffer at room temperature according to the manufacturer’s protocol (Biolegend). Cells were co-stained with DAPI at a final concentration of 1 µg/ml for 2 minutes in Annexin binding buffer ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with Annexin V-FITC (5uL in 0.14 mL Annexin V Binding Buffer, #640914; Biolegend, San Diego, CA) for 10 minutes at 4°C in darkness ...
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bioRxiv - Immunology 2023Quote: ... washed once in Annexin V buffer and resuspended in Annexin V binding buffer with Annexin V-BV421 (Biolegend; 640924) and incubated for 15 minutes at room temperature protected from the light ...
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bioRxiv - Immunology 2022Quote: ... to block nonspecific binding and then were stained with dead dye to exclude dead cells (Aqua, ThermoFisher or Zombie NIR, Biolegend) for 15 min at 4 °C ...