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Citations for BioLegend :
201 - 250 of 3190 citations for Glypican 3 GPC3 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Fc-blocked (#158002, Biolegend), then stained for surface markers (CD45 APC #157606 and CD4 FITC #100406 ...
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bioRxiv - Immunology 2021Quote: ... The cells were then stained with PE-conjugated anti-Human IgG Fc antibody (clone M1310G05, BioLegend 410720, San Diego, CA, USA) to detect bound anti-CTLA-4 and with fluorescein isothiocyanate (FITC)-conjugated anti-CD27 (clone O323,
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bioRxiv - Bioengineering 2020Quote: ... cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-cMyc antibody and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with an Alexa Fluor 647-labeled mouse monoclonal antibody against the human IgG1-Fc (Biolegend, San Diego, USA, cat #409320) and analyzed in a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Genomics 2021Quote: Both iMGs and oMGs were resuspended in staining buffer and were blocked with Fc receptor blocking antibody (Human TruStain FcX, BioLegend 422302) for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-cMyc antibody and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... Blocking and antibody solutions were further supplemented with 1:150 Human TruStain FcX™ Fc Receptor Blocking Solution (BioLegend, London, UK), to reduce non-specific binding ...
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bioRxiv - Immunology 2023Quote: ... for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX, BioLegend, San Diego, California) in PBS with 1% BSA for 10 min at 4C ...
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bioRxiv - Immunology 2023Quote: ... for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX, Biolegend, San Diego, California) in PBS with 1% BSA for 10 min at 4C ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2022Quote: ... tissue sections were blocked with 3% BSA and Human TruStain FcX (1:100, BioLegend) in PBST for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416, Biolegend) for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor was blocked with an anti-Fc receptor antibody (BioLegend). The cells were stained with a cocktail of antibodies against three surface makers ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were blocked using an Fc blocking antibody (Biolegend Fc-block clone: 2.4G2) and lymphocytes were stained with CD3 and CD4 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2021Quote: ... 3) mouse anti-human CD196 prelabeled with Alexa Fluor 488 (CCR6 marker; clone G034E3, BioLegend) and 4 ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with Fc Block (BioLegend) then with fluorochrome-conjugated antibodies (BioLegend unless noted ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG Fc (Biolegend; M1310G05), anti-CD27 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocking reagent (Biolegend, 422301) was used to block Fc receptor-introduced non-specific staining ...
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bioRxiv - Microbiology 2022Quote: ... CD16/32 (Fc block-Biolegend), B220 (AF700-Biolegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptor blockade (Biolegend 101319), and anti-CD31 antibody (BD Biosciences 561073) ...
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bioRxiv - Genomics 2024Quote: ... Fc-blocking reagent (BioLegend, 422301) was used to block Fc receptor-introduced nonspecific staining ...
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bioRxiv - Microbiology 2023Quote: ... Fc receptors were blocked with 0.5% anti-CD16/32 Fc block antibody (Biolegend, clone 93) diluted in PBS containing 2% goat serum (Vector Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... coverslips of each batch were incubated with 30 µg/ml anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (Cat# 409321; BioLegend, San Diego, CA) for 45 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... infected or stimulated cells using a LEGENDplex Human Type 1/2/3 Interferon Panel assay (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Developmental Biology 2021Quote: ... and then Fc blocked (BioLegend, 101320), before staining with an antibody cocktail containing anti-MPO (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... and Fc receptor blocking solution (Biolegend), before staining with fluorochrome-conjugated anti-human antibodies specific to anti-CD3 (UCHT1)/anti-CD45 (2D1) ...
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bioRxiv - Neuroscience 2022Quote: ... After fragment crystallizable (Fc) blocking (Biolegend), cells were stained for surface antigens (Extended Data Table 4) ...
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bioRxiv - Cancer Biology 2020Quote: ... After adding Fc receptor blocker (BioLegend), cells were labeled overnight at 4°C with a cocktail of antibodies recognizing chromatin modifications or intracellular components ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block (BioLegend), followed by tetramer incubation (25μg/ml ...
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bioRxiv - Immunology 2022Quote: ... 5µg/ml CD80-Fc (555404, Biolegend), and 2µg/ml PD-L1-Fc (758206 ...
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bioRxiv - Immunology 2022Quote: ... stained with Fc receptor block (BioLegend) for 10 minutes at 4°C ...