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Citations for BioLegend :
151 - 200 of 7101 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... To exclude dead cells Zombie Aqua Fixable Viability Kit (#423102, 1:100, BioLegend) was added ...
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bioRxiv - Immunology 2022Quote: ... was counted in an automated Vicell cell counter before 2X106 cells/well were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FBS and 10ng/ml Macrophage colony- stimulating factor (M-CSF) (Biolegend), (growth media ...
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bioRxiv - Microbiology 2019Quote: ... After fixation and permeabilization, intracellular cytokine staining was performed with fluorescent monoclonal antibody against IFNγ (Phycoerythrin, eBioscience) and TNFα (Allophycocyanin, BioLegend). Cells were analyzed by flow cytometry on a BD LSR II flow-cytometer (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Neuroscience 2022Quote: ... samples that passed visual inspection were subjected to flow cytometry with the fluorescent nuclear stain DRAQ7TM (Biolegend, San Diego, CA) and an Alexa FluorTM 488-conjugated antibody against the neuronal intermediate filament beta-tubulin III (TuJ1) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2024Quote: ... NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend, 10ug/ml) or with IL-12 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... was used according to manufacturer’s recommendations: intracellular labeling with APC anti-GATA3 antibody (2.5 ul antibody per million cells in 100 ul) (Biolegend #653806) and PE anti-DYKDDDDK Tag antibody (0.6 ul antibody per million cells in 100 ul ...
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bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
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bioRxiv - Cancer Biology 2023Quote: ... Harvested cells were incubated in Dulbecco’ s Modified Eagle Medium (DMEM) with a cell activation cocktail with brefeldin A (Biolegend) for 4 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... or co-stimulated with plate-bound CD3 (Biolegend #300333) and soluble CD28 (Biolegend #302943 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were removed from their plates with Accutase (BioLegend) and counted ...
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bioRxiv - Immunology 2021Quote: ... AMs were lifted from plates using Accutase™ (BioLegend) and seeded for experiments.
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bioRxiv - Cell Biology 2021Quote: ... Azide-Free (LEAF) plate-bound anti-mouse CD3 (Biolegend) was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated overnight at 4°C with either r3CLpro-RBD chimer (2 μg/mL) ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated with r3CL-pro ...
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bioRxiv - Immunology 2022Quote: ... Plates were again washed and TMB substrate solution (BioLegend) was added for 15 minutes at room temperature prior to addition of stop solution (2M hydrochloric acid) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and developed using TMB substrate (BioLegend). Reaction was stopped using 50μL of H2SO4 ...
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bioRxiv - Microbiology 2023Quote: ... coated onto ELISA plates (BioLegend, San Diego, CA, USA) at a final protein concentration of 100 ng/well and incubated at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... plate-bound antibodies (Abs) anti-NK1.1 (clone PK136; Biolegend), anti-NKG2D (clone A10 ...
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bioRxiv - Immunology 2023Quote: ... Plates were developed with 50 μl TMB (421101, BioLegend) and stopped using 50 μl 1.5 N H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... stimulated with plate-bound anti-CD3 (Biolegend, 1μg/ml) and anti-CD28 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2021Quote: ... fixed for 1 h at 25 °C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Immunology 2022Quote: ... fixed for 1 h at 25°C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cytokine levels were determined using the human inflammation panel 1 LEGENDplex™ kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... T cells were pelleted in a V-bottom plate and resuspended in 40 µl PBS with 2% BSA and fluorescent 9V pMHC tetramers that were produced with Streptavidin-PE (Biolegend, 405204) and used at a predetermined dilution (1:100-1:1000) ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Cancer Biology 2023Quote: ... Later 25µl of the following mixture of fluorescent-labeled antibodies was added to the samples: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... were isolated from buffy coats by density gradient centrifugation (Lymphoprep, StemCell ref: 07851) and activated on plates coated with anti-CD3 (1 mg/ml, Biolegend, ref: 317347, clone: OKT3) and anti-CD28 (1 mg/ml ...
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bioRxiv - Biophysics 2023Quote: ... The gel substrate for T cells had a Young’s modulus of 1.2 kPa and was coated with 5 μg/mL each of anti-CD3 (OKT3; 317326; BioLegend, CA, USA) and anti-CD28 (CD28.6 ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... assays were performed using the in situ red starter kit according to the manufacture’s protocol with JADE1 antisera (1:20) and 0N tau antisera (1:500; BioLegend, San Diego, CA). Two control assays were also performed ...
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bioRxiv - Genomics 2020Quote: ... Expression of cell surface markers in resting and CD3/CD28 activated cells was made by flow cytometry using fluorescent-coupled antibodies against CD4 (Biolegend, Cat: 100434), CD3 (Biolegend ...
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Immunology 2021Quote: Dead cells were stained using either Zombie UV™ Fixable Viability Kit (Biolegend, 1:1000), Fixable Viability Dye eFluor™ 506 (eBioscience ...
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bioRxiv - Immunology 2020Quote: Human ILC2 cells were stained with 1 μM CSFE (CSFE cell division tracker kit, BioLegend) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit, Biolegend, 1:500), CD45-AP/Cyanine7 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cell viability was determined using either Zombie Aqua™ Fixable Viability Kit (1:800, Biolegend) or LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (1:1,000) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were seeded in CD3-coated plates (clone OKT3; Biolegend) and cultured in medium (RPMI1640 with 10 % FBS ...
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bioRxiv - Bioengineering 2020Quote: ... ELISA plates were coated with anti-IL-2 Ab (BioLegend) at a concentration of 0.25ug/ml overnight at 4 °C or 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and then the TMB substrate solution (Biolegend) was added ...
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bioRxiv - Microbiology 2023Quote: ... the plates were incubated with TMB (3,3’,5,5’-Tetramethylbenzidine) (BioLegend) substrate for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... plate-bound anti-CD3 antibody (5μg/mL; Biolegend or BD), IL-1β (50ng/mL ...
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bioRxiv - Immunology 2023Quote: ... and plate-bound anti-CD3 antibody (5 μg/mL; Biolegend). For the induction of IL-22-expressing Th17 cells ...
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bioRxiv - Immunology 2023Quote: ... the plate was coated with anti-CD3 (clone 17A2, BioLegend) and anti-CD28 (clone 37.51 ...