Labshake search
Citations for BioLegend :
501 - 550 of 7197 citations for Epiandrosterone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... Plates were washed and incubated with goat IgG anti-mouse IgA-HRP (BioLegend). Plates were washed and developed in the dark using TMB substrate ...
-
bioRxiv - Microbiology 2020Quote: ... The cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml) ...
-
bioRxiv - Immunology 2020Quote: ... T cell activation was performed using plate-bound α-CD3 (clone OKT3, Biolegend) and α-CD28 (clone 9.3 ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were restimulated on plates coated with anti-CD3 and anti-CD28 (BioLegend). After 48 hours of restimulation ...
-
bioRxiv - Immunology 2022Quote: ... TCR stimulation was provided by plate-bound αCD3 (clone HIT3a, BioLegend, cat#300313) at 1μg/mL unless otherwise specified ...
-
bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with 100 μL/well TMB-substrate (Biolegend/ITK). The substrate reacted approximately 8 minutes ...
-
bioRxiv - Immunology 2022Quote: ... T cells were activated with plate-bound anti-CD3ε (clone 145-2C11; Biolegend) and 0.5 µg/ml of soluble anti-CD28 (clone 37.51 ...
-
bioRxiv - Bioengineering 2024Quote: ... Plates were then washed with PBS before the addition of 1x streptavidin (Biolegend) in PBS + 0.5% BSA (w/v ...
-
bioRxiv - Bioengineering 2024Quote: ... and then activated by pre-coated plate-bound anti-CD3 (1μg/ml; Biolegend) and soluble anti-CD28 ((1μg/ml ...
-
bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/ml anti-mouse-IgM or IgG (Biolegend) in NaHCO3 0.1 M pH 9.6 ...
-
bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
-
bioRxiv - Immunology 2021Quote: 96-well plates (Nunc, MaxiSorp, flat bottom, uncoated, BioLegend, San Diego, CA, USA, 423501) were coated with 0.5 μg/mL rhCTLA-4-Fc chimera (7268-CT ...
-
bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated with 5 g/ml of anti-CD28 antibody (Biolegend) o/n at 4°C to provide a co-stimulatory signal during co-culture ...
-
bioRxiv - Cancer Biology 2019Quote: ... and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend, CA ...
-
bioRxiv - Immunology 2022Quote: ... After the plate was washed five times and a 3,3′,5,5′-Tetramethylbenzidine solution (Biolegend) was prepared and incubated for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333) with media containing soluble CD28 (Biolegend #302943 ...
-
bioRxiv - Immunology 2020Quote: ... - plates were coated with Goat anti-mouse IgG (clone Poly4053 at 10μg/ml - BioLegend) for 1 hour at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
-
bioRxiv - Immunology 2023Quote: ... the plate was further incubated with HRP-conjugated goat anti-mouse IgG (poly4053, Biolegend) at 1:20,000 for 1 h before development with TMB Substrate Set (421101 ...
-
bioRxiv - Immunology 2023Quote: ... T cells were incubated in 96 well plates with Brefeldin A (15µg/mL, Biolegend) and stimulated with PMA (5ng/mL ...
-
bioRxiv - Immunology 2024Quote: Plates were coated with 1.25 µg/ml anti-CD3 (145-2C11 or OKT3, Biolegend) with or without 10 µg/ml Fc proteins (R&D Systems Inc.) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
-
bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
-
bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
-
bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were either stained with DAPI for viability or fixed with 1% PFA and stained with zombie aqua fixable viability kit (Biolegend). Viable cells were sorted on a FACSAria flow cytometer (Becton Dickinson) ...
-
bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).
-
bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
-
bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
-
bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
-
bioRxiv - Biochemistry 2023Quote: ... was added 1 day after serum deprivation and cell viability was assessed the following day by using Zombie Fixable Viability Kit (Biolegend) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... Serum samples were prepared for Legendplex assays by diluting 1:50000 for the detection of immunoglobulin isotypes using the mouse immunoglobulin isotyping kit (BioLegend). The assay was performed in a V-bottom plate and data was acquired on a BD Fortessa X-30 cytometer (BD Biosciences) ...
-
bioRxiv - Genetics 2023Quote: ... Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-on a FACSAria into StemSpan SFEM II media with SCF (100 ng/ml, BioLegend), TPO (50 ng/ml ...
-
bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
-
bioRxiv - Cancer Biology 2021Quote: ... c-Kit (BioLegend), EpCam (G8.8 ...
-
bioRxiv - Immunology 2022Quote: ... tissue culture plates were coated with 5 μg/mL of anti-human CD162 (KPL, Biolegend). On day three of activation ...
-
bioRxiv - Cancer Biology 2019Quote: ... The isolated CD8+ T cells were cultured on plates coated with anti-mouse-CD3 (BioLegend) in RPMI-1640 containing 10% FBS ...
-
bioRxiv - Immunology 2021Quote: ... Plates were washed three times and incubated ∼7min with 50 µl of TMB substrate (BioLegend). The reaction was stopped with 25µl of 1M phosphoric acid (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were stimulated with plate bound αCD3 and αCD28 (both 1μg/ml, Biolegend). After 12 hours ...
-
bioRxiv - Immunology 2021Quote: ... Cells were activated in a 24-well plate coated with anti-CD3 (clon: 500A2, Biolegend) and soluble anti-CD28 (clon ...
-
bioRxiv - Immunology 2020Quote: ... tissue-culture plates were coated with 5 μg/mL purified anti-CD3 (clone HIT3a, Biolegend) at 4°C overnight and subsequently washed twice with 1X PBS ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed and 50 μl/well of TMB High Sensitivity substrate solution (BioLegend, UK) was added ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cultured in 96-well plates coated with 1µg/ml anti-CD3 (BioLegend, Clone UCHT1) or control mIgG (BioLegend ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs were activated on plates coated with anti-CD3 (1mg/ml, Biolegend, #317347, clone: OKT3) and anti-CD28 (1mg/ml ...
-
bioRxiv - Immunology 2022Quote: ... The plate was washed five times with PBST and developed with tetramethylbenzidine substrate (Biolegend, 421101). After 20 min at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... Plates were washed 3X with PBST before adding HRP goat anti-mouse antibody (BioLegend 405306) at a 1:10,000 dilution in PBST ...
-
bioRxiv - Immunology 2023Quote: ... 24-well plates have been coated with 1μg purified human TCRγ/δ antibody (331202, BioLegend) per well overnight at 4 °C ...