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Citations for BioLegend :
1 - 50 of 65 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... BSA/0.01% (v/v) Tween-20 in cold RNase-free PBS + 10 μl Human TruStain FcX blocking solution (#422301, BioLegend)) and incubated for 10 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining 80% cleared supernatant was transferred to a clean RNase-free tube and incubated with mouse anti-HA antibody (1:250; clone 16B12, Biolegend) on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads ...
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bioRxiv - Immunology 2024Quote: Recombinant mouse IFNγ (carrier-free, BioLegend) was reconstituted in sterile double distilled water and freshly diluted in 1X Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Immunology 2021Quote: ... Carrier-free recombinant mouse IL-1α (Biolegend) was locally administered at a dose of 1 μg / 10 μL per day ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant Human IFNα2 (carrier free) (Biolegend, #592702) were purchased for MCF10A experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Azide-Free (LEAF) plate-bound anti-mouse CD3 (Biolegend) was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Immunology 2024Quote: Carrier-free recombinant mouse IL-33 (Biolegend; Catalog #: 580504) was diluted with sterile PBS to prepare a 7.5 µg/ml solution ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant mouse CX3CL1 carrier-free for intracerebroventricular (i.c.v.) administration (BioLegend), and with BSA as a carrier protein to enhance protein stability for in-vitro studies (R&D Systems ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human IL-2 (carrier-free) was purchased from BioLegend. Human Immune Checkpoint Array C1 was purchased from RayBiotech ...
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bioRxiv - Bioengineering 2024Quote: ... and free active TGF-β1) (BioLegend, San Diego, CA, USA) was used to quantify the secreted cytokines in the cell culture supernatant following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... All mAbs were low endotoxin azide-free (Biolegend custom, LEAF specification). Fully functionalized DMSs were washed in sterile PBS and washed once again in the cell culture media to be used for the T cell expansion ...
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bioRxiv - Immunology 2024Quote: ... was purchased from Bioxcell and recombinant mouse IL-2 (carrier free, cat # 575408 from Biolegend). IL-2/anti-IL-2 complexes were prepared by mixing both reagents ...
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Intrinsically Disordered Regions Define Unique Protein Interaction Networks in CHD Family RemodelersbioRxiv - Biochemistry 2024Quote: ... and mouse erythropoietin (EPO, carrier-free, 2 U/mL, #587608, BioLegend). Cells were maintained in a humidified incubator with 5% CO2 at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: Continuous intraperitoneal IFNα delivery (IFNα1 carrier-free, Biolegend, San Diego, CA, USA) was achieved by intraperitoneal implantation of mini-osmotic pumps (MOP ...
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bioRxiv - Physiology 2023Quote: ... with or without EPO (35U/well) (recombinant mouse EPO carrier-free, Biolegend). The ratio of splenocytes to RBCs was 1:1 ...
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bioRxiv - Immunology 2021Quote: ... B16-F1 and A375 cells were treated using carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Bioengineering 2023Quote: ... GM-CSF (20 ng/mL; recombinant Mouse GM-CSF (carrier-free) from BioLegend) ...
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bioRxiv - Bioengineering 2023Quote: ... GM-CSF (20 ng/mL; recombinant Mouse GM-CSF (carrier-free) from BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with pre-warmed serum-free media and TGF-β (Biolegend) was added in a final concentration of 1 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... and 100ng/mL final concentration of recombinant human Flt3L (carrier-free formulation, BioLegend). Plates were briefly centrifuged and then imaged at 6-hour intervals for mCherryNLS fluorescence using the BioTek Cytation 7 Cell Imaging Multimode Reader (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
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bioRxiv - Immunology 2023Quote: ... Recombinant Mouse GM-CSF (carrier-free; 20 ng/ml; BioLegend, San Diego, California, USA), 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... the same lysates were processed using LEGENDplex™ Mouse/Rat Free Active/Total TGF-β1 Assay (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Organoids were harvested in cold Serum-Free Defined Medium media and incubated with Zombie (BioLegend 423107, 1:100) for 15 minutes in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... freshly sorted cells were stained ex vivo or after stimulation with carrier-free recombinant mouse IL-25 (Biolegend) at a concentration of 100 ng/ml in complete RPMI medium for 36 h ...
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bioRxiv - Immunology 2024Quote: ... mice were injected intraperitoneally (i.p.) with 0.2mg/kg low endotoxin/azide-free (LEAF) anti-mouse CD41 (MwReg30) or IgG1κ control (both Biolegend) and harvested 12-24hrs later ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatants were collected followed by ELISA to detect IL-10 and TGFβ using capture and detecting antibodies (Biolegend). Other M2 phenotype characteristics including the cell surface expressions of CD206 ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT buffer and supernatants were collected for analysis using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1.
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: Secretion of soluble cytokines and chemokines were assayed from cell-free culture supernatants of in vitro stimulated neutrophils and monocytes using bead-based immunoassays (LegendPlex, Biolegend). Two assays were used allowing the quantification of IL-1β ...
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bioRxiv - Immunology 2023Quote: ... After blocking of free capture sites on the biosensors with an isotype control (10 µg/mL, human IgG1κ, clone QA16A12, BioLegend) for 600 s ...
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bioRxiv - Immunology 2023Quote: ... Tgf-β concentration was measured using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1 ...
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bioRxiv - Cell Biology 2024Quote: ... Excess media was eluted off and replaced with serum-free media containing typically 250 nM MitoSpyTM Red CMXRos (424801, BioLegend)(in some experiments MitoTracker was used instead ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2020Quote: Active TGF-β1 serum levels in patients and healthy donors were measured using LEGEND MAX™ Free Active TGF-β1 ELISA Kit with Pre-coated Plates (Biolegend). The assay was run according to the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2023Quote: ... in phenol-red free DMEM-F12 in combination with live-dead stain Zombie NIR (1: 10,000 dilution; Biolegend, cat. no 423106) and incubated for 25 minutes at 37 °C and 5% CO2 conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... and conditioned for 16h at 37 °C with cell-free AKPS-supernatant (1:5) or the following mouse cytokines at a concentration of 20ng/µl: TGF-β1 (BioLegend, 763102), IL-10 (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... Sorted LSK cells were cultured in StemSpan SFEM serum free media (stemcell technologies) contains 40 ng/ml mSCF (Biolegend, Cat. No. 579702), 40 ng/ml mTPO (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... or restimulated on day 11 in 96-well plates with 2.5ug/ml recombinant SARS-CoV-S protein S1 (Biolegend 792906, carrier-free) for 8h in the presence of 1ug/ml Golgiplug and 0.7ug/ml Golgistop ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Then cells were fixed with 4 % methanol-free formaldehyde in HBSS for 10 min prior to staining with either 4 μg/ml anti-ACE2 (Biolegend, clone A20069I) or anti-NRP-1 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... were rehydrated in distilled H2O then stained with Nissl or immunohistochemical labelling was performed as above with anti-mouse Ly6G primary antibody (BioLegend, London, UK) and goat anti-rabbit secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... Recombinant mouse IL-10 ([vehicle solution water] 1ng/ml, Biolegend) was added overnight before infection ...
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bioRxiv - Neuroscience 2023Quote: ... 1X Intracellular Staining Perm Wash Buffer (diluted in water from Biolegend, cat no. 421002) was used to permeabilize cells and as the diluent and wash buffer for intracellular epitopes ...