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Citations for BioLegend :
301 - 350 of 8114 citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Brilliant Violet 711™ anti-mouse/human CD11b antibody (1:200; Biolegend, M1/70), Brilliant Violet 421™ anti-mouse F4/80 antibody(1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Cell Biology 2023Quote: ... was added 1:200 and Alexa fluor 647 anti-human/mouse CD49f (Biolegend, 313610) was added 1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... was added 1:200 and Alexa fluor 647 anti-human/mouse CD49f (Biolegend, 313610) was added 1:250 ...
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bioRxiv - Microbiology 2023Quote: ... Cytokine levels were determined using the human inflammation panel 1 LEGENDplex™ kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used for human staining were anti-FoxP3 PE (Biolegend, Cat: 320108, 1:100), anti-CD3 APC (BD ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then pelleted and stained with anti-human CD45–BV605 (1:100; BioLegend), anti-human CD31–BV421 (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa Fluor® 647 mouse/human anti-SSEA1 antibodies (1:200, Biolegend; 125608) at 4°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... The following FACS antibodies were used at 1:200: mouse anti-human CD45 APC-Cy7 (BioLegend; Cat#304014; used at 1:200), mouse anti-human anti-CD11b-APC-Cy7 (BD ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Bioengineering 2021Quote: ... The His tagged ACE2 variants were detected using anti-His mAb (Cat. No. 652505, BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then resuspended in 0.2µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG, Jackson Immunoresearch 1:200, Brilliant Violet 421-anti-Rabbit IgG, Biolegend 1:200). This was incubated for 10-60 min before cells were washed as above and resuspended for acquisition on a BD LSRII or Beckman Coulter Cytoflex S ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Immunology 2021Quote: ... PE anti-human-CD8 a (Clone RPA-T8; dilution 1:200; Biolegend; Cat no 301008); FITC anti-human CD20 (clone 2H7 ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of PE anti-human IgG Fc antibody (clone HP6017, BioLegend, Cat. No. 409304) buffer-exchanged into 1x PBS was added to yeast ...
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bioRxiv - Cell Biology 2021Quote: ... Human conjugated antibodies used for assessing engraftment and differentiation derived from Biolegend (1:100 dilution); huCD45-Brv421 (368522) ...
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bioRxiv - Genetics 2021Quote: ... The cell suspension was incubated in Human TruStain FcX blocking solution (1:20, Biolegend; 422302) at room temperature for 10 minutes ...
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bioRxiv - Immunology 2020Quote: Human ILC2 cells were stained with 1 μM CSFE (CSFE cell division tracker kit, BioLegend) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... and 1×106 cells were incubated sequentially or together with Human Trustain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406) ...
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bioRxiv - Immunology 2020Quote: ... with 100ug of antibodies against specific human NK cells expressing NKp46 Domain 1 (Biolegend, 9E2) and/or human CD8α (Bioxcell ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies were used at 1:1000 dilution: mouse anti-human CD63 (clone H5C6, Biolegend); mouse anti-human CD81 (TAPA-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human CD3 Antibody (#300322, Mouse IgG2a κ, dilution 1:100, Biolegend); APC Rat IgG2a κ Isotype Ctrl (#400512 ...
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bioRxiv - Cell Biology 2022Quote: ... PE Rat anti-Mouse/Human CD44 (Monoclonal; clone IM7; Biolegend; 1:100; Cat#103007, FACS). BUV395 Rat anti-Mouse CD45 (Monoclonal ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was analyzed using the LEGENDPlex™ Assay (BioLegend; Human Inflammation Panel 1 (13-plex)) as per manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with anti-human IgG-APC (clone M1310G05, 1/250 dilution, BioLegend) in PBS-BSA for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in 50µL ice-cold 1:10 human TruStain FcX (BioLegend 422302) in MACS buffer and incubated for at least 5 minutes on ice ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of Human TruStain FcX™ Fc Receptor Blocking Solution (1:400, BioLegend), and fixed with 0.5 mL methanol-free paraformaldehyde 4% (Thermo Scientific™ Pierce™ ...
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bioRxiv - Immunology 2024Quote: ... Donor 1 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... 550 µl of RPMI medium supplemented with 1 µg/ml recombinant human CCL21 (BioLegend; #582208) or nothing as negative control were added to the lower chambers ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4) (BioLegend; Cat. No. 306514) for CRISPRa validation or 1:66 PE-Cy7 anti-human CD71 (TFRC ...
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bioRxiv - Neuroscience 2022Quote: ... for CRISPRa validation or 1:66 PE-Cy7 anti-human CD71 (TFRC) (BioLegend; Cat. No. 334112) for CRISPRi validation for 30 min in the dark ...
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bioRxiv - Biophysics 2020Quote: ... USA) and PE-conjugated anti-human CD66a/c/e (dilution 1:40, #34303, BioLegend, CA, USA) in MB for 25 min at 600 rpm in a block thermomixer (Thermomixer comfort 5355 ...
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bioRxiv - Cell Biology 2019Quote: THP-1 cells were labeled with mouse antibody anti-human CD18 (PE BioLegend or BV421 BD) (1 µl per 200 000 cells ...
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bioRxiv - Bioengineering 2021Quote: ... such as the recombinant human matrix metalloproteinases 12 (MMP12, 1 μl, 20 ng/ml, Biolegend, U.S.A), MMP408 (the inhibitor of MMP12 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with monoclonal mouse anti-human Lamp-1-AlexaFluor488 (BioLegend 328610, clone H4A3) at a final dilution of 1:500 for 1 hours ...
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bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...