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Citations for BioLegend :
701 - 750 of 1080 citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with an antibody that recognizes the luminal epitope of the human LAMP1 protein (clone H4A3, BioLegend) conjugated with Alexa fluor 488 or 647 for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Pathology 2022Quote: ... was coated with 1.2 μg/mL HERV-K113 Capsid protein (sp|Q902F9|90-632, Genescript) diluted in ELISA coating buffer (421701, Biolegend) at 4 ℃ overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... Experimental values are expressed as a ratio of protein levels over control with the TFIIB (Biolegend, 1:2000 dilution) and Histone H3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... staining and neutrophil purity was assessed using a Benton Dickson LSR Fortessa X-20 flow cytometer via distinct forward and side scatter profiles or CD66b-peridinin chlorophyll protein (PerCP)/Cy5.5 (clone G10F5, BioLegend) expression ...
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Genomics 2021Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend 101251, clone M1/70), CD45-APC (BioLegend 103112 ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Immunology 2023Quote: ... a 10X molar excess of biotinylated RBD-Avi protein was incubated with 0.5 mg/ml of streptavidin-AlexaFluor 488 or streptavidin-AlexaFluor 647 (BioLegend) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The expression level of specific proteins was detected by immunoblot analysis with the following antibodies: HA (BioLegend, MMS-101R), V5 (Thermo Fisher Scientific R96025) ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 stimulation group (SPT stim) with hashtag 2 (BioLegend, Cat# 155803); spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 594 or Alexa Fluor 647 conjugated anti-mouse CD31 (2 μg/mL; clone MEC13.3; BioLegend) was used ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... and cells were instead stained with 4′,6-diamidino-2-phenylindole (DAPI; BioLegend, San Diego, CA, USA) and gated for live (DAPI- ...
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bioRxiv - Immunology 2021Quote: ... IFN-β and IFN-γ were determined using the LEGENDplex mouse type 1/2 interferon panel (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... as well as SARS-CoV-2-S1 (biotin-coupled; Acro; #S1N-C82E8) and fluorochrome-coupled streptavidin (Biolegend). The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 2 μl of anti-human CD3-AF488 (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE (clone 61D3 ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-centrifuged 50 μL of hashtag antibody solution containing 2 uL of TotalSeq-A Hashtag antibody (BioLegend), with a unique barcode sequence for each iPS cell line ...
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bioRxiv - Physiology 2022Quote: ... mice were intravenously injected with 2 μg of Alexa Fluor 647 anti-mouse CD127 antibody (A7R34, BioLegend) and euthanized 5 minutes after injection ...
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bioRxiv - Immunology 2022Quote: ... The abundance of Nectin-2+ monocytes and NK cell intracellular cytokine production of IFN-γ (4S.B3, Biolegend) were analyzed by flow cytometry (BD LSRFortessa™ Cell Analyzer ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Immunology 2022Quote: Cells were stained for flow cytometry using the following antibodies diluted in FACS Buffer (2% FBS, 0.01% Sodium Azide, PBS): BV711-anti-CD326 (EpCAM) (Clone:G8.8, BioLegend), BUV395-anti-CD45.2 (Clone:104 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2020Quote: ... anti-human BDCA-2-PE (clone AC144; Miltenyi Biotech) and anti-human CD123-APC (clone 6H6; Biolegend). Cells were incubated with surface antibodies at 4°C for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Immunology 2020Quote: ... or 2) 10 μg/mL αLFA-1 + 10 μg/mL antibodies against CD16 (αCD16; BioLegend, clone 3G8). All loading solutions were mixed in PBS and supplemented with 10 μg/mL bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... CD8 BUV496 or BV650 (2:100 or 4:100; BD Biosciences Cat# 612942 or Biolegend Cat# 301042), and Live/Dead eFluor506 (5:1000 ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated for 2 days with 10 ug/ml of anti-CD40 (Biolegend, Cat#102812) and 10 ng/ml of rec ...
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bioRxiv - Immunology 2022Quote: ... in the presence of rhIL-2 (50U/ml, Novartis) and anti-IFNγ (5µg/ml, clone XMG1.2, Biolegend). For intracellular cytokine staining ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Physiology 2022Quote: ... Samples were stained with 3 μM of (4’,6-Diamidino-2-Phenylindole, Dilactate (DAPI, Biolegend Cat# 422801)) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: ... cells were activated at ∼2-4M cells/ml for 8 hrs using CD3 antibody (Biolegend clone OKT3) coated tissue culture plates ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2) (Biolegend, San Diego CA) viability dye and blocked using 2% FBS ...
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bioRxiv - Immunology 2023Quote: ... aliquots of conditioned media were collected for measuring IL-2 and IFNγ by ELISA (ELISA MAX, BioLegend). Cells were washed and stained with anti-mouse CD3ε-FITC (145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... serum samples were diluted 2-fold with assay buffer and standards were mixed with matrix solution (Biolegend) to account for additional components in the serum samples ...