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Citations for BioLegend :
51 - 100 of 8287 citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 μg of PE anti-human IgG Fc antibody (clone HP6017, BioLegend, Cat. No. 409304) buffer-exchanged into 1x PBS was added to yeast ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of Human TruStain FcX™ Fc Receptor Blocking Solution (1:400, BioLegend), and fixed with 0.5 mL methanol-free paraformaldehyde 4% (Thermo Scientific™ Pierce™ ...
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bioRxiv - Immunology 2021Quote: ... mouse ICAM-1-Fc and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1-Fc was from R&D Systems (Minneapolis ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Human TruStain FcX™ (Biolegend), and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone ...
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bioRxiv - Immunology 2021Quote: ... and Fc blocked for 10 minutes (Human TruStain FcX, Biolegend), and incubated with a cocktail containing CD3-PE (SP34 ...
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bioRxiv - Immunology 2021Quote: ... and after Fc receptor blocking with Human TruStain FcX (Biolegend), cells were stained with the following antibodies in FACS buffer (PBS with 2 % fetal calf serum ...
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bioRxiv - Cancer Biology 2021Quote: ... and Human TruStain FcX™ Fc Receptor Blocking Solution (BioLegend). The stained samples were then incubated and stained with Alexa Fluor® 700 anti-human CD45 Antibody (BioLegend) ...
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bioRxiv - Cell Biology 2020Quote: ... cells pre-incubated with human Fc-receptor blocking solution (BioLegend) to reduce nonspecific staining carried out at a concentration of (0.01% ...
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bioRxiv - Immunology 2020Quote: ... Direct-Blot HRP anti-human IgG1 Fc antibody (Biolegend 410604) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and Fc blocked for 10 min (Human TruStain FcX, Biolegend), and incubated with a cocktail containing CD3 (SP34 ...
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bioRxiv - Immunology 2022Quote: ... blocked with Human TruStain FcX Fc receptor blocking solution (BioLegend) for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... Human TruStain FcX™ (Fc Receptor Blocking Solution, 422302, BioLegend) was used at 1:100 dilution for blocking nonspecific binding ...
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bioRxiv - Cancer Biology 2024Quote: ... and after Fc receptor blocking with Human TruStain FcX (Biolegend), cells were stained with the following antibodies in FACS buffer (PBS with 2% fetal calf serum ...
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Immunology 2024Quote: ... Human cells were blocked by Human TruStain FcX Fc receptor blocking solution (#422301 from BioLegend). Flow cytometry analysis was performed using an AccuriC6 Plus platform ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... human and mouse FC receptors were blocked using Human TruStain FcX (BioLegend, San Diego, CA, USA) and purified anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend, Table S6) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... APC-conjugated anti-human IgG Fc (clone HP6017) was from Biolegend. For analysis of CD47 expression on human PBMCs ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD4 (BV711 ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD3 (APC-Cy7 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with Fc Receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes prior to staining with TotalSeq C antibodies (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG Fc allophycocyanin (APC) (BioLegend, San Diego, CA, USA) was added as a secondary antibody at 10 μg/mL and the plate was incubated at 4°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with Fc blocking reagent (Biolegend Human Trustain FcX) for 15 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The remaining cells underwent anti-human Fc-blockade (Biolegend, TruStain FcX) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: Single cell suspensions were blocked with human BD Fc Block (BioLegend) for 5 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody used was anti-human IgG Fc antibody (Biolegend, 410720). All the respective isotype antibodies used were procured and used as per the vendor’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using an APC Anti-Human IgG Fc (Biolegend) at a 1:100 dilution incubated for 1 hr with the bacteria ...
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bioRxiv - Bioengineering 2022Quote: ... then stained with APC anti-human IgG Fc Antibody clone HP6017 (Biolegend). Samples were acquired on a BD FACSymphony A5 analyzer and data were analyzed using FlowJo (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Pooled barcoded samples were treated with FC-block (Human TruStain FcX, Biolegend) for 10 minutes then surface antibody stained for 30 minutes in cell staining medium (PBS with 0.5% BSA and 0.02% sodium azide) ...
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bioRxiv - Genomics 2021Quote: ... and incubated with Fc-receptor blocking antibody (Human TruStain FcX, BioLegend 422302) in homogenization buffer for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR-NK cells were loaded with human recombinant PD-L1- Fc (Biolegend) or ErBb2-Fc fusion proteins (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with Fc receptor blocking antibodies (Human TruStain FcX, Biolegend) 1:20 in Cell Staining Buffer (CSB ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... In all assays 1 x 106 cells were first incubated with Human TruStain FcX (Fc Receptor Blocking Solution, Biolegend) or anti-human CD32 (FcγRII Blocker ...
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bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
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bioRxiv - Immunology 2022Quote: ... pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend, San Diego, CA). As a negative control ...
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bioRxiv - Cell Biology 2022Quote: ... The single-cell suspension was resuspended in Fc-Block (Human TruStain FcX, BioLegend) and incubated with CD31 microbeads (Miltenyi Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... washed and incubated for 10 min with human Fc TruStain FcX (Biolegend #101320). Cells were stained for surface antigens ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were incubated with Fc Receptor Blocking Solution (Human TruStain FcX, Biolegend) according to the manufacturer’s instructions for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... added with 5 µl of Human TruStain FcX™ Fc Blocking reagent (Biolegend). Cell suspensions were then incubated at 4°C for 10 mins ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with 5 µL Fc-block (Human TruStain FcX, Biolegend 422302) and 1.5 µL of S167-180 HLA class II PE-conjugated tetramer for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 50 µL FACS containing 5 µL human Fc-block (Biolegend, 422302), and blocked for 15 min at RT ...