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1 - 50 of 94 citations for DSPE PEG 2000 Amine since 2019

• Functional spreading of hyperexcitability induced by human and synthetic intracellular Aβ oligomers

  Eduardo J. Fernandez-Perez, et al., bioRxiv - Neuroscience 2020

  Quote: ... amine-reactive resin was coupled with anti-Aβ 6E10 antibody (BioLegend, USA) followed by incubation with PBS-soluble fraction of AD brain homogenate ...
• Didemnin B and ternatin-4 inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes

  Manuel F. Juette, et al., bioRxiv - Biochemistry 2022

  Quote: ... and fixable live/dead stained with Zombie Red amine-reactive dye (BioLegend) according to the manufacturer’s instructions ...
• Longitudinal single cell transcriptional and epigenetic mapping of effector, memory, and exhausted CD8 T cells reveals shared biological circuits across distinct cell fates

  Josephine R. Giles, et al., bioRxiv - Immunology 2022

  Quote: ... Cells were washed with PBS and stained with an amine–reactive dye (Biolegend #423104) for 20 minutes to assess cell viability ...
• Longitudinal single cell transcriptional and epigenetic mapping of effector, memory, and exhausted CD8 T cells reveals shared biological circuits across distinct cell fates

  Josephine R. Giles, et al., bioRxiv - Immunology 2022

  Quote: ... Cells were washed with 1× PBS and stained with an amine–reactive dye (Biolegend # 423106) for 20 minutes at room temperature to assess cell viability ...
• Establishment of CD1b-restricted immunity to lipid antigens in the pulmonary response to Mycobacterium tuberculosis infection

  Macallister C. Harris, et al., bioRxiv - Immunology 2023

  Quote: ... Viability of cells was assessed via Zombie Yellow amine-reactive permeability dye (BioLegend, Cat# 423103). Data were acquired using a spectral unmixing Cytek Aurora flow cytometer (Cytek ...
• Chronic morphine administration differentially modulates viral reservoirs in SIVmac251 infected rhesus macaque model

  Arpan Acharya, et al., bioRxiv - Molecular Biology 2020

  Quote: ... After that 1/1000 dilution of zombie aqua amine reactive dye (Biolegend, San Diego, CA; cat# 423101) was added to the cell suspensions and incubated for 30 minutes in the dark ...
• Chemokines form complex signals during inflammation and disease that can be decoded by extracellular matrix proteoglycans

  Amanda JL Ridley, et al., bioRxiv - Immunology 2022

  Quote: ... Cells were then incubated with a Live/Dead amine reactive viability dye (Zombie ultraviolet (UV) dye) (BioLegend) diluted 1:2000 in 10 μl 1X PBS for 15 min at RT in the dark to facilitate dead cell exclusion ...
• BRAWNIN: A sORF-encoded Peptide Essential for Vertebrate Mitochondrial Complex III Assembly

  Shan Zhang, et al., bioRxiv - Cell Biology 2020

  Quote: ... 1:2000 (BioLegend, 637302); anti-C4ORF48 (Abcam ...
• Myeloid Tribbles 1 induces early atherosclerosis via enhanced foam cell expansion

  Jessica M Johnston, et al., bioRxiv - Immunology 2019

  Quote: ... Approximately 106 cells/sample were re-suspended in PBS and dead cells removed using amine-reactive dye NIR Zombie (1:500 dilution in the antibody master mix; #423105, BioLegend). Live cells were stained with the following cell surface marker specific antibodies ...
• BDNF-dependent modulation of axonal transport is selectively impaired in ALS

  Andrew P. Tosolini, et al., bioRxiv - Neuroscience 2021

  Quote: ... p75NTR (Biolegend, 839701, 1:2000), ERK1/2 (CST ...
• Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner

  Sun-Hee Hwang, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Pax6 (1:2000, 901301; Biolegend), Pax7 (1:10 ...
• The deubiquitinase USP9X regulates RIT1 protein abundance and oncogenic phenotypes

  Amanda K. Riley, et al., bioRxiv - Cancer Biology 2023

  Quote: ... HA 1:2000 (Biolegend, 901503).
• Pathological mutations reveal the key role of the cytosolic iRhom2 N-terminus for phosphorylation-independent 14-3-3 interaction and ADAM17 binding, stability, and activity

  Katharina Bläsius, et al., bioRxiv - Molecular Biology 2023

  Quote: ... αHA (1:2000; Biolegend; 901502), αTransferrin-receptor (1:2000 ...
• Genetic effects on brain traits impact cell-type specific gene regulation during neurogenesis

  Nil Aygün, et al., bioRxiv - Genetics 2021

  Quote: ... TUJ1 (1:2000, mouse, Biolegend #801202), Alexa Fluor 568 (1:1000 ...
• Oxidative Modifications of Parkin Underlie its Selective Neuroprotection in Adult Human Brain

  Jacqueline M. Tokarew, et al., bioRxiv - Neuroscience 2020

  Quote: ... clone E (BioLegend, A15165E; 1:2000), and clone G (1:250) ...
• Human cortical expansion involves diversification and specialization of supragranular intratelencephalic-projecting neurons

  Jim Berg, et al., bioRxiv - Neuroscience 2020

  Quote: ... SMI-32 (Biolegend #801704, 1:2000); GFAP (Millipore #MAB360 ...
• Age-Associated Insolubility of Parkin in Human Midbrain is Linked to Redox Balance and Sequestration of Reactive Dopamine Metabolites

  Jacqueline M Tokarew, et al., bioRxiv - Neuroscience 2020

  Quote: ... clone E (BioLegend, A15165E; 1:2000), and clone G (1:250) ...
• Monocarboxylate transporter 1 in Schwann cells is critical for maintenance of sensory nerve myelination during aging

  Mithilesh Kumar Jha, et al., bioRxiv - Neuroscience 2019

  Quote: ... or CD271 (p75NTR; BioLegend, 1:2000) either alone or in combination ...
• The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity

  Predrag Jevtic, et al., bioRxiv - Cell Biology 2019

  Quote: ... mAb414 (BioLegend, # 902901, mouse, 1:2000), Nup133 (Santa Cruz Biotechnology ...
• Familial Alzheimer’s disease mutation undermines axonal transport by enhancing dynactin recruitment to the APP motor assemblies

  Monica Feole, Gorazd B. Stokin, bioRxiv - Neuroscience 2022

  Quote: ... pNFH (mouse 1:2000, BioLegend 801601). TurboRFP (1:1000 ...
• Propionate reinforces epithelial identity and reduces aggressiveness of non-small cell lung carcinoma via chromatin remodelling

  Vignesh Ramesh, et al., bioRxiv - Cancer Biology 2023

  Quote: ... γH2AX (1:2000, mouse, Biolegend, 613402), RAD51 (1:1000 ...
• Structure and dynamics of a pentameric KCTD5/Cullin3/Gβγ E3 ubiquitin ligase complex

  Duc Minh Nguyen, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-HA (1/2000, #901501, BioLegend) and anti-b-tubulin (1/20000 ...
• Glucocerebrosidase deficiency leads to neuropathology via cellular immune activation

  Evelyn S. Vincow, et al., bioRxiv - Genetics 2023

  Quote: ... 1:2000 mouse anti-HA (BioLegend), 1:1000 mouse anti-mCherry (Invitrogen) ...
• BAX and BAK expression triggers multiple IRE1 signaling outputs under ER stress

  Claudia Sepulveda, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-HA (1:2000; 901514, Biolegend) anti-IRE1alpha (1:1000 ...
• Neuroprotection of low dose carbon monoxide in Parkinson’s disease models commensurate with the reduced risk of Parkinson’s among smokers

  KN Rose, et al., bioRxiv - Neuroscience 2024

  Quote: ... NeuN (BioLegend cat# 834501, 1:2000), alpha-synuclein (ThermoFisher cat# 32-8100 ...
• A feedback loop between heterochromatin and the nucleopore complex controls germ-cell to oocyte transition during Drosophila oogenesis

  Kahini Sarkar, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Mouse anti-NPC (1:2000; BioLegend, AB_2565026) and Rat anti-HA (1:500 ...
• MUCOSECRETORY LUNG DISEASE: DIFFERENT ASSEMBLIES OF JAG1 AND JAG2 DETERMINE TRACHEOBRONCHIAL CELL FATE

  Susan D. Reynolds, et al., bioRxiv - Cell Biology 2022

  Quote: ... and Keratin 5 (BioLegend #905501, 1:2000).
• Aberrant cortical development is driven by impaired cell cycle and translational control in a DDX3X syndrome model

  Mariah L. Hoye, et al., bioRxiv - Developmental Biology 2022

  Quote: ... mouse: anti-TUJ1 (Biolegend, 801202, 1:2000), anti-CTIP2 (Abcam ...
• YAP/TAZ Maintain the Proliferative Capacity and Structural Organization of Radial Glial Cells During Brain Development

  Alfonso Lavado, et al., bioRxiv - Developmental Biology 2021

  Quote: ... rabbit anti-PAX6 (BioLegend #901301, 1:2000) and mouse anti-TBR2-APC (MACS #130-102-378 ...
• White matter changes in the perforant path in patients with amyotrophic lateral sclerosis

  Jeroen Mollink, et al., bioRxiv - Neuroscience 2019

  Quote: ... and SMI-312 (1:2000, BioLegend, 837901) were incubated for 1 hour at room temperature ...
• Continuous cis-regulatory changes in an advantageous gene are linked with adaptive radiation in cichlid fishes

  Langyu Gu, et al., bioRxiv - Evolutionary Biology 2021

  Quote: ... Mouse anti-HA (901501 Biolegend, 1:2000) and mouse anti-GAPDH (60004-1-lg Proteintech ...
• Proteomic interrogation of the pathogen-host interface in cholera

  Abdelrahim Zoued, et al., bioRxiv - Microbiology 2021

  Quote: ... anti-RNA Polymerase (1:2000, Biolegend, 663903) and anti-OmpU (1:500 ...
• The exon junction complex component EIF4A3 is essential for mouse and human cortical progenitor mitosis and neurogenesis

  Bianca M. Lupan, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and Tuj1 (TUBB3, Biolegend, 801202, 1:2000).
• Allogeneic Tumor Cell-Derived Extracellular Vesicles Stimulate CD8 T Cell Response in Colorectal Cancer

  Travis J Gates, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and ALIX (cat# 634502, Biolegend 1:2000) were used to validate TEV protein markers ...
• Arl3 regulates ODA16-mediated intraflagellar transport in motile cilia biogenesis

  Yameng Huang, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-HA (mouse, BioLegend, 901501, 1:2000), anti-Protein C (mouse ...
• Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis

  Noor M. Kotb, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Mouse anti-NPC (1:2000; BioLegend, AB_2565026). The following secondary antibodies were used ...
• Inhibition of microtubule detyrosination by parthenolide facilitates functional CNS axon regeneration

  Marco Leibinger, et al., bioRxiv - Neuroscience 2023

  Quote: ... and βIII-tubulin (1:2000; BioLegend, RRID:AB_2313773) at 4°C overnight ...
• A novel approach to evaluate alpha-synuclein seeding shows a wide heterogeneity in multiple system atrophy

  Ivan Martinez-Valbuena, et al., bioRxiv - Neuroscience 2021

  Quote: ... nitrated anti-α-synuclein (Syn514; 1:2000; Biolegend), C-terminal truncated x-122 anti-α-synuclein (A15127A ...
• 3D-imaging reveals conserved cerebrospinal fluid drainage via meningeal lymphatic vasculature in mice and humans

  Laurent Jacob, et al., bioRxiv - Neuroscience 2022

  Quote: ... Rabbit anti-mouse TUJ1 (802001, Biolegend; 1:2000), Rabbit anti–human vWF (A0082 ...
• BNP facilitates NMB-mediated histaminergic itch via NPRC-NMBR crosstalk

  Qing-Tao Meng, et al., bioRxiv - Neuroscience 2021

  Quote: ... rabbit anti-βIII-Tubulin (1:2000, Biolegend, 802001), rabbit anti-GFP (1:1000 ...
• Loss of IRF5 increases ribosome biogenesis leading to alterations in mammary gland architecture and metastasis

  Zarina Brune, et al., bioRxiv - Cancer Biology 2023

  Quote: CK5 (Biolegend, #905501, 1.0 mg/mL, 1:2000)
• Mimicking the breast metastatic microenvironment: characterization of a novel syngeneic model of HER2⁺ breast cancer

  Aaron G. Baugh, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-CK5 (1:2000 dilution; BioLegend, cat. 905501), anti-CK6 (1:200 dilution ...
• “Non-viral CRISPR/Cas9 Mutagenesis for Streamlined Generation of Mouse Lung Cancer Models.”

  Irene Lara-Saez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Keratin 5 (PRB-160P, BioLegend, 1:2000); anti-CC10 H-75 (sc-25554 ...
• Early-stage idiopathic pulmonary fibrosis is characterized by bronchoalveolar accumulation of SPP1⁺ macrophages

  Jiangyan Yu, et al., bioRxiv - Immunology 2023

  Quote: ... cells were stained with DAPI (1:2000, BioLegend) for 20 min at room temperature and later were washed 3 times with PBS ...
• A ubiquitin-based mechanism for the oligogenic inheritance of heterotaxy and heart defects

  Jennifer H. Kong, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-HA.11 (clone 16B12, BioLegend, 1:2000); mouse anti-HA (clone 2-2.2.14 ...
• Investigation of USP30 inhibition to enhance Parkin-mediated mitophagy: tools and approaches

  Eliona Tsefou, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-Beta III tubulin (Biolegend, 801201, 1 in 2000), anti-GFAP (CST ...
• A general role for MIA3/TANGO1 in secretory pathway organization and function

  Janine McCaughey, et al., bioRxiv - Cell Biology 2021

  Quote: ... rabbit polyclonal anti-giantin (dilution 1:2000, Poly19243, BioLegend), 0.25 μg.ml-1 mouse monoclonal anti-GM130 (610823 ...
• Regulation potential of transcribed simple repeated sequences in developing neurons

  Tek Hong Chung, et al., bioRxiv - Genomics 2023

  Quote: ... and anti-MAP2 (Biolegend, cat# 822501; 1:2000 dilution) antibodies in the IF blocking buffer at 4°C overnight ...
• Comprehensive characterization of the neurogenic and neuroprotective action of a novel TrkB agonist using mouse and human stem cell models of Alzheimer’s Disease

  Despoina Charou, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... Cells were then immunostained (1:2000, Biolegend, cat# 801201) against Tuj1 and imaged using confocal microscopy ...
• Apoptotic signaling clears engineered Salmonella in an organ-specific manner

  Taylor J. Abele, et al., bioRxiv - Immunology 2023

  Quote: ... or HA (1:2000, mouse, Biolegend, Cat. MMS-101R). Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies ...