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Citations for BioLegend :
701 - 750 of 1944 citations for Creatinine Serum Kit 4 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... HTO staining was carried out with 0.5μg of β2M and CD298 antibody-conjugated oligonucleotides (Total-seq B Hastags 4, 6 and 8; BioLegend) with ∼500,000-750,000 isolated single cells ...
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bioRxiv - Microbiology 2022Quote: ... and stained intracellularly for 30 minutes in PBS with antibodies including: PE anti-mouse IL-4 antibody (Biolegend) or PE anti-mouse IFN-γ antibody (Biolegend) ...
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bioRxiv - Developmental Biology 2020Quote: Freshly isolated mammary cells were incubated at 4°C for 20 min with the following antibodies from Biolegend: anti-CD24-BViolet421 (clone M1/69 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Immunology 2020Quote: ... The following directly conjugated antibodies were used in these experiments: CD4 Alexa Fluor 700 (Clone RM4-4, BioLegend), CD4 BUV737 (Clone GK1.5 ...
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4 °C and followed by incubation with Streptavidin conjugated to Alexa 488 (1:200, Biolegend, #405235) and an anti-mouse secondary conjugated (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with the corresponding dilutions of the antibodies CD45 (BioLegend, clone 30-F11) and CD31 (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2021Quote: ... The following directly conjugated antibodies were used in these experiments: CD4 Alexa Fluor 700 (Clone RM4-4, BioLegend), TCRβ Alexa Fluor 700 (clone H57-597 ...
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bioRxiv - Immunology 2021Quote: ... Cells were also stained to measure activation with the following markers: CD137 APC (4:100; Biolegend Cat# 309810), OX40 PE-Cy7 (2:100 ...
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bioRxiv - Immunology 2022Quote: ... 3-4 × 106 splenocytes after cell surface staining were fixed with Cyto-Fast Fix/Perm buffer (BioLegend, USA) for 20 min at room temperature and washed twice with FACS staining buffer ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... for 15 minutes at 4°C followed by incubation with AntiHA.11 Epitope Tag Affinity Matrix (BioLegend 900801) for 4 hours at 4°C under constant rotation ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Th17 differentiation media consisted of complete media supplemented with 2 μg/mL anti-IL-4 (Cat# 504101; Biolegend), 2 μg/mL anti-IFNγ (Cat# BE0055 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Cancer Biology 2021Quote: 96-well round-bottom plates were coated with either 2.5 µg/mL anti-CD3 (Biolegend, clone 145-2C11,Cat# 100359) or HIgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... splenocytes from OT-II/Rag1/CD45.1 mice were cultured in a-CD28 coated plates (Biolegend, Cat. No. 102115, 1ug/ml) with complete RPMI (10%FBS ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 1×105 TILs were seeded per well of a round bottom 96 well plate and stimulated with phorbol-12-myristate-13-acetate/Ionomycin in the presence of Brefeldin A (Biolegend) for 4 hours as per manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... The cells were activated in 96-well round bottom plates that were coated with 3ul/ml anti-CD3e (BioLegend #100202) and 5ul/ml anti-CD28 (BioLegend #102102 ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used for coating of culture plates for in vitro T cell activation: anti-human CD3 (clone HIT3α; Biolegend), anti-human CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded at a density of 2.5 × 104/well in 96-well plates pre-coated with 1ug/ml CD3 (Biolegend100339) and 1ug/ml CD28 (Biolegend 102115) antibodies and cultured in 1640 media with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred to a non-CD3 coated plate and incubated for an additional three days before activation with Cell Activation cocktail with Brefeldin A (Biolegend) for 6 h and collection ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed four times with PBS-T and developed with 100 µL/well TMB High Sensitivity substrate solution (BioLegend, UK). After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor cell lines were plated in triplicate on 96-well plates and treated for 72 hours with 10 ng/mL TNF (Biolegend) or 0.1% BSA vehicle control ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB High Sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB high sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transferred to a round-bottomed 96-well plate and were incubated with an AF647-conjugated anti-GFP antibody (1:20, BioLegend) and fixable viability dye eFluor 780 (1:1000 ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Immunology 2023Quote: ... the appropriate number of wells of a flat-bottomed tissue culture plate were coated with 10 μg/mL αCD3 (Biolegend) in PBS for 3h at 37°C ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells of a 24-well plate were coated with 1 μg/mL anti-CD3 (#317326) and anti-CD28 antibodies (#302934, both BioLegend). For activation of T-cells ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected cells were then stimulated for 24h at 37°C with plate bound 1μg/ml of anti-CD3 (clone UCHT1, BioLegend #300402) and 2μg/ml of anti-CD28 (clone CD28.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...