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Citations for BioLegend :
1 - 50 of 291 citations for Corning Lymphocyte Serum Free Medium since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Lymphocytes (all BioLegend): Live-Dead Brilliant Violet 450 (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... Organoids were harvested in cold Serum-Free Defined Medium media and incubated with Zombie (BioLegend 423107, 1:100) for 15 minutes in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: Skin CD8+ lymphocytes labeled with TotalSeqC hashtag antibodies (BioLegend) from S ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes were labelled with Zombie Aqua viability dye (423102, BioLegend), anti-CD45 ...
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bioRxiv - Bioengineering 2024Quote: ... Lymphocyte panel specific antibodies included NKp46 (Brilliant Violet 650, BioLegend, Clone# 29A1.4 ...
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bioRxiv - Immunology 2021Quote: Lymphocytes in the PBMC were gated as CD45+ (BioLegend cat. 304024), live cells (Zombie red-negative ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with pre-warmed serum-free media and TGF-β (Biolegend) was added in a final concentration of 1 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... lymphocytes were analyzed using True Nuclear transcription factor buffer (Biolegend, Dedham-MA). Permeabilized cells were stained with antibodies specific for T-bet and EOMES ...
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bioRxiv - Immunology 2021Quote: ... Lymphocytes were stained for anti-human HLA-A2 (clone BB7.2, BioLegend, 343324), CCR7 (clone G043H7 ...
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bioRxiv - Immunology 2023Quote: ... lymphocytes were analyzed using True Nuclear transcription factor buffer (Biolegend, Dedham-MA). Permeabilized cells were stained with antibodies specific for T-bet and EOMES ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were stained using fixable viability dye eFluor 780 (eBioscience) or Zombie UV (Biolegend), anti-CD3 (145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis and early apoptosis were detected by staining the lymphocytes with 7AAD and Annexin V (Biolegend) or NucView® Caspase-3 Substrate (Biotium ...
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bioRxiv - Cancer Biology 2024Quote: ... After that the lymphocytes were and blocked by Mice TruStain FcX (Fc Receptor Blocking Solution, BioLegend) for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... intracranial and systemic lymphocyte samples were stained with anti-human CTLA-4 antibody (Biolegend, clone BNI3, BV421). Additionally ...
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bioRxiv - Immunology 2024Quote: MMP and pHi were determined by staining lymphocytes with the MMP indicator MitoSpy Orange (Biolegend, San Francisco, CA) and the pH indicator pHrodoTM Green/Red AM (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Cell Biology 2024Quote: ... Excess media was eluted off and replaced with serum-free media containing typically 250 nM MitoSpyTM Red CMXRos (424801, BioLegend)(in some experiments MitoTracker was used instead ...
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bioRxiv - Microbiology 2022Quote: ... total lymphocytes were counted and naïve B cells were isolated using Mojosort Naïve B cell isolation beads (Biolegend 480068) or Naïve B cell Isolation Kit II (Miltenyi 130-091-150 ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Microbiology 2024Quote: ... Human CD4+ T lymphocytes were isolated from PBMCs using the MojoSort Human CD4+ T Cell Isolation Kit (BioLegend, 480010) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometric analysis of lymphocyte subset percentage was performed in lung and collected blood by using commercially labelled CD4+ (Cat≠100412; Biolegend): ...
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bioRxiv - Microbiology 2022Quote: ... For baseline T cell frequencies 0.5(10)6 cells from baseline uninfected total lymphocyte samples were stained and analyzed as above with phenotype antibody panel as follows: CD95-APC (2μl, Biolegend 305611), CCR7-PE (2μl ...
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bioRxiv - Immunology 2020Quote: ... For baseline T cell frequencies 0.5e6 cells from baseline uninfected total lymphocyte samples were stained and analyzed as above with phenotype antibody panel as follows: CD95-APC (2μl, Biolegend 305611), CCR7-PE (2μl ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T lymphocytes were purified (> 98%) by negative selection with MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3 ...
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bioRxiv - Cancer Biology 2022Quote: ... the phenotype of live T-/CAR T-lymphocytes in all the experimental conditions was assessed using the following antibodies: BV510-anti CD3 (clone OKT3, BioLegend), APC-CY7-anti CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2020Quote: ... total lymphocytes were counted and Naïve B cells were isolated using Mojosort™ Naïve B cell isolation beads (Biolegend 480068) or Naïve B cell Isolation Kit II (Miltenyi 130-091-150 ...
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bioRxiv - Immunology 2021Quote: ... lymphocytes were stained in 100 μl PBS with a viability dye (Zombie Aqua™ Fixable Viability Kit, Biolegend, the Netherlands) for 15 min at RT in the dark ...
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bioRxiv - Immunology 2023Quote: CD4+ and CD8+ T cells were isolated from the spleen lymphocytes of healthy mice using MojoSort mouse CD4 nanobeads and a MojoSort mouse CD8α selection kit (BioLegend), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Single cell lymphocyte suspensions were isolated as described above and stained with anti-CD45-APC (Biolegend 103112, clone 30-F11), anti-CD3e-PE (Biolegend 100308 ...
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bioRxiv - Cell Biology 2021Quote: ... Sorted LSK cells were cultured in StemSpan SFEM serum free media (stemcell technologies) contains 40 ng/ml mSCF (Biolegend, Cat. No. 579702), 40 ng/ml mTPO (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD3+ CAR-T cells were then purified from lymphocytes using MojoSort™ Mouse CD3 T Cell Isolation Kit according to the manufacturer’s instructions (BioLegend, 480031). CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were incubated in high glucose Dulbecco’s’ modified Eagle medium (DMEM) (Capricorn) supplemented with 10% fetal bovine serum (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney epithelial) cells consisted of high glucose Dulbecco’s modified Eagle medium (DMEM) (Capricorn) with 10 % fetal bovine serum (FBS) (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Immunology 2023Quote: Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Immunology 2020Quote: A total of 1×106 splenocytes or liver lymphocytes were surface stained with Brilliant Violet (BV) 421-conjugated anti-CD107a (clone 1D4B, BioLegend, San Diego, CA) during the 24 h stimulation ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The lymphocytes were collected at the interface and washed before stained by biotinylated BSA-fentanyl and then AF647-streptavidin (BioLegend, San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... Carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Neuroscience 2023Quote: The single-cell suspension was stained at 4°C for 15 minutes with antibodies recognizing lymphocytes and endothelial cells (anti-CD45/CD31; BioLegend Way, San Diego, CA, USA) and labeling live cells (CellTrace calcein green ...
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bioRxiv - Immunology 2024Quote: Recombinant mouse IFNγ (carrier-free, BioLegend) was reconstituted in sterile double distilled water and freshly diluted in 1X Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Immunology 2021Quote: ... Carrier-free recombinant mouse IL-1α (Biolegend) was locally administered at a dose of 1 μg / 10 μL per day ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant Human IFNα2 (carrier free) (Biolegend, #592702) were purchased for MCF10A experiments ...
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bioRxiv - Microbiology 2023Quote: ... 2% (v/v) Goat-serum (Biolegend) and 0.05% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Azide-Free (LEAF) plate-bound anti-mouse CD3 (Biolegend) was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Immunology 2024Quote: Carrier-free recombinant mouse IL-33 (Biolegend; Catalog #: 580504) was diluted with sterile PBS to prepare a 7.5 µg/ml solution ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 2% Normal Serum Block (BioLegend), 1% BSA ...