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Citations for BioLegend :
51 - 91 of 91 citations for Canine Distemper Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed according to manufacturer’s instructions for staining intracellular antigens (eBioscience) or using the True-Nuclear Transcription Factor Buffer Set (BioLegend). Flow cytometric analysis was performed using a FACS FORTESSA and FlowJo softwares (Tree Star ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were quantified by a fluorescence-encoded bead-based multiplex assay (LEGENDplex Mouse Anti-Virus Response Panel, BioLegend), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cells from lineage-depleted apheresis or NK92 cells were incubated on cells or cell-free matrix for 30 minutes in the presence of directly conjugated antibodies to surface antigens CD56 (1:50, clone HCD56, Biolegend) and CD29 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2020Quote: ... The following surface antigens were detected by the indicated mAb clone: B220 (RA3-6B2-BV785 and RA3-6B2-BV421, BioLegend), CD21 (7EG-PerCP-Cy5.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Except for immunohistochemistry for SOX-9 in which a β-gal staining was performed at 37°C for 3h followed by fixation with PFA 4% for 30 min at RT and incubation with unmasking antigen solution (927901; Biolegend). Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40 ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analyzed using the LEGENDplex™ Human Anti-Virus Response Panel multiplex assay (BioLegend, San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend).
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bioRxiv - Genomics 2020Quote: ... Cells were then washed once with full staining buffer (3% FBS in PBS with 2 mM EDTA) and surface antigens were stained for 30 min on ice using the following antibodies: gdTCR-BV421 (B1, Biolegend, 331218), CD45-Pacific Blue (HI30 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with rat polyclonal T antigen antibody (1:500) followed by an anti-rat secondary (Biolegend, 1:200). For measuring sialic acid binding dependence ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were live-stained for two hours using a conjugated antibody targeted against an OVA257-264 peptide antigen (BioLegend, 141605). The cells were then fixed with 4% paraformaldehyde and imaged using the PerkinElmer Operetta CLS High-Content Analysis System ...
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bioRxiv - Immunology 2020Quote: ... YU2-gp140 fold-on, and hepatitis B surface antigen (HBs Ag) (Protein Specialists, hbs-875) conjugated to streptavidin Alexa Fluor 647 (Biolegend, 405237), streptavidin PE (BD biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... a multiplex cytokine quantification (LEGENDplex HU Anti-Virus Response Panel (13-plex) and LEGENDplex HU Th Cytokine Panel (13-plex) (cat# 740390 and cat# 740722, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and was analyzed with the DropArray (Curiox Biosystems) and the MAGPIX System (Luminex) or with LEGENDPlex Human Anti-Virus Response Panel (13-plex, BioLegend) and was analyzed with LSRII (BD ...
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bioRxiv - Immunology 2021Quote: Fluorophore- or biotin-conjugated antibodies specific for human cell-surface antigens and cytokines were as follows: anti-CD71 (CY1G4, BioLegend, DF1513, NovusBio), anti-CD235a (HI264 ...
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bioRxiv - Immunology 2020Quote: ... OVA and HEL were biotinylated in house to ∼1 biotin per antigen molecule and tetramers constructed as described [14] using streptavidin-PE (Biolegend, CA, USA), streptavidin-APC (Biolegend ...
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bioRxiv - Microbiology 2022Quote: Corning ELISA plate wells were coated with 100µL of 100 μg/mL FCoV2 whole virus antigen or 100 μg/mL SCoV2 RBD or FCoV RBD antigen in sodium bicarbonate ELISA coating buffer at pH 9.5 (BioLegend, San Diego, CA) and incubated overnight at RT ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with 1x Perm buffer (Tonbo) and stained for intracellular antigens for 30 mins at 4°C with the following antibodies: GranzymeB: AF647 (Biolegend, Clone: GB11), IL-2 ...
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bioRxiv - Biochemistry 2019Quote: ... After transfection cell supernatants were collected and subjected to cytokine production analysis using LEGENDplex™ Mouse Anti-Virus Response Panel (13-plex) (BioLegend) on BD FACS Canto II flow cytometer using BD FACSDiva 8.0 software for acquisition and BioLegend’s LEGENDplex™ Data Analysis Software for data analysis.
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to the staining of 332 surface antigens using LEGENDScreen Human Cell Screening PE Kit (cat.no. 700001; Biolegend, San Diego, CA, USA). Data acquisition was performed on FACSVerse (Becton Dickinson (BD) ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated with cognate antigens for three days and then supernatants were collected for IL-17A and IFNγ detection with ELISA (Biolegend, San Diego, CA). For determination of IL-17A+ and IFNγ+ CD45.1+ OT-II CD4+ T cell percentages ...
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bioRxiv - Molecular Biology 2023Quote: ... and spleen were counted on a Pentra ES60 Haematology Analyser (Horiba) and stained with anti-mouse antibodies against the following antigens as appropriate (all from BioLegend unless otherwise stated) in FACS buffer (PBS with 2% BSA and 0.025% sodium azide) ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
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bioRxiv - Immunology 2022Quote: ... 5 x 105 cells from each sample were stained for combinatorial hashing using oligonucleotide-conjugated Hashtag antibodies targeting the ubiquitous cell surface antigens Beta-2-Microglobulin and CD298 (Biolegend Totalseq A Hashtag antibodies). Hashed samples were pooled and stained together with a cocktail of 73 oligonucleotide-conjugated antibodies targeting cell surface antigens ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were labelled with antibodies against cell surface antigens as described above and then prepared using a Cyto-Fast Fix/Perm kit (BioLegend, San Diego, CA, US), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The levels of serum cytokines and chemokines were determined by a bead-based multiplex assay using the LEGENDplex mouse anti-virus response panel (13-plex) kit (#740622, Biolegend, San Diego, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Apical supernatant from cells at each harvest time point was assessed using LEGENDPlex Human Anti-Virus Response Panel (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions and using a FACSCanto II flow cytometer (BD Biosciences ...