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Citations for BioLegend :
151 - 200 of 2705 citations for CD28 Human Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Anti-CD3 (clone 2c11) and anti-CD28 (clone 37.51) were from BioLegend. Goat anti-Armenian Hamster antibody was from Jackson ImmunoResearch.
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bioRxiv - Cell Biology 2021Quote: ... was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend) was used at 8 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51, BioLegend) under Th1-polarizing conditions [1 ng/mL mouse IL-2 (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... CD28-PE-Cy7 (clone CD28.2) and CXCR3-BV605 (clone G025H7) from Biolegend. Cells were permeabilized with Cytofix/Cytoperm Kit (BD Biosciences ...
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bioRxiv - Physiology 2019Quote: ... and anti-CD28 (1 µg/mL) (Biolegend, catalog #504701, 100201, and 102101) were added to maintain a CD4+CD25+ Treg population ...
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bioRxiv - Immunology 2020Quote: ... in the presence of 1μg/ml anti-CD28 (clone CD28.2, BioLegend, USA) and 10mg/ml Brefeldin A (Cayman Chemical ...
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bioRxiv - Immunology 2020Quote: Purified anti-CD3ε (145–2C11) and anti-CD28 (37.51; both from Biolegend) were used at the appropriate concentration for mouse T cell activation ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 μg/ml soluble anti-mouse CD28 antibody (102116, BioLegend, USA) in RPMI 1640 medium (21870-076 ...
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bioRxiv - Immunology 2021Quote: ... and Ultra LEAF-purified anti-CD28 (clone 37.51, Biolegend cat. no. 102116) was used as a soluble stimulator.
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bioRxiv - Immunology 2021Quote: ... Stimulatory anti-CD3 (OKT3) and anti-CD28 (CD28.2) antibodies were from BioLegend. Flow cytometry was performed on a FACSymphony or LSR II (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and APC anti-His Tag antibody (Biolegend) for 30 min each ...
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bioRxiv - Immunology 2019Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ...
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bioRxiv - Immunology 2022Quote: Self-made α-CD3ε (clone UCHT1) and α-CD28 (clone CD28.2) from BioLegend were used for γδ T cell stimulation.
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bioRxiv - Cell Biology 2021Quote: ... anti-mouse CD3 (145-2C11) and anti-mouse CD28 (37.51) were from BioLegend. Anti-human CD3 (OKT3 ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2, Biolegend). The CEM-A cell line was obtained through NIH AIDS Reagent Program ...
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bioRxiv - Neuroscience 2022Quote: ... cells were restimulated on plates coated with anti-CD3 and anti-CD28 (BioLegend). After 48 hours of restimulation ...
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bioRxiv - Immunology 2022Quote: ... alone or in combination with 2ug/mL biotinylated anti-CD28 (Clone 37,15, BioLegend) for 15min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 1 μg/mL biotinylated anti-CD28 antibody (Clone 37.15, BioLegend) PBS+2%FCS for 30 minutes on ice to allow binding ...
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bioRxiv - Immunology 2023Quote: ... and then incubated with CD28 Monoclonal Antibody (CD28.2) (302934, BioLegend; 0.5 μL/well), CD49d (Integrin alpha 4 ...
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bioRxiv - Immunology 2024Quote: ... T cell activation was initiated using 0.5 μg/mL anti-mouse CD28 (BioLegend) and 10 ng/mL recombinant mouse interleukin-2 (IL-2 ...
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bioRxiv - Immunology 2024Quote: ... along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122) for a duration of 2 days ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
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bioRxiv - Immunology 2020Quote: ... APC-anti-his tag antibody (Biolegend, clone: J095G46)) ...
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bioRxiv - Biochemistry 2022Quote: ... and Purified anti-His Tag antibody (BioLegend 652502) for 1 hour in blocking buffer shaking at rt ...
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bioRxiv - Microbiology 2023Quote: ... Phycoerythrin (PE) anti-His tag antibody (BioLegend 362603) was added to the cell suspension at a dilution of 1:20 and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated with 5 g/ml of anti-CD28 antibody (Biolegend) o/n at 4°C to provide a co-stimulatory signal during co-culture ...
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bioRxiv - Genomics 2022Quote: ... Naïve CD8a+ T cells were activated with anti-CD3ε and anti-CD28 antibodies (BioLegend), For in vitro experiments ...
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bioRxiv - Immunology 2021Quote: ... CD28-peridinin chlorophyll protein (PerCP) Cy5.5 (clone CD28.2; Cat#302922, BioLegend, San Diego, CA), CD95-fluorescein isothiocyanate (FITC ...
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bioRxiv - Immunology 2022Quote: ... T cell activation antibodies– purified anti-CD3ε and purified anti-CD28 (BioLegend, #100340, 102116). For all experiments ...
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bioRxiv - Immunology 2022Quote: ... A non-differentiated control group (Th0) was treated with anti-CD28 Ab and rhIL2 (Biolegend) alone ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse CD28 antibody (Clone: 37.51, Biolegend, Cat#102116, 1 μg/mL in medium) for 5-7h in the presence of Brefeldin A (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... CD3χ (145-2C11; 100340) and CD28 (35.51; 102112) were purchased from Biolegend (San Diego, CA). Antibody for S6 (pS235/pS236 ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were coated with 3.33 μg/mL α-CD3 (HITa3) and α-CD28 antibodies (Biolegend), in 100 μL PBS at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Then resuspend and count in 1640 complete medium containing CD28 (5 μg/ml, 302934, Biolegend) and IL-2 (10 ng/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Two microliters of mouse anti-hexa His antibody (BioLegend) were added to the 10 mL sample and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 100μl of HRP anti-His Tag Antibody (BioLegend, USA) (diluted 1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a HRP anti-His tag antibody (Biolegend, #652504) diluted 1:1000 in Superblock (Thermo #37515 ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated anti-His tag secondary antibody (BioLegend, 652503) diluted 1:2000 in blocking buffer was then added to the plates and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG ...
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bioRxiv - Bioengineering 2024Quote: ... we used HRP anti-His tag antibody (BioLegend 652503).
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bioRxiv - Cancer Biology 2021Quote: ... The same amount of PBMCs incubated with CD3/CD28 beads and PMA/ionomycin (Biolegend, Cat. # 423301) were used as a positive control ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 1 μg/mL anti-CD28 and 1 ng/mL recombinant IL7 (BioLegend) for 48 hours ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used in each sample were: mouse anti-HA 1:2000 (Biolegend, 901513; HEK293), rabbit anti Cav2.3 N-terminus 1:250 (Covalab ...
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bioRxiv - Immunology 2023Quote: Human: Anti-human CD141 (clone M80, BioLegend), anti-human CD45RA (clone HI100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified anti-His tag mouse antibody was from BioLegend (USA). Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... incubating the cells with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... For T-cell receptor (TCR) activation studies anti-CD3∊ (OKT3) and CD28 (CD28.2) antibodies were from BioLegend, and light chain specific goat anti-mouse IgG (115-005-174 ...