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Citations for BioLegend :
601 - 650 of 4335 citations for CD16 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... RBD was then detected using an Alexa Fluor 488 conjugated anti-His antibody (clone J099B1, Biolegend). Cells were analyzed on a flow cytometry (BD).
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bioRxiv - Biochemistry 2022Quote: ... one slide was probed with Alexa Fluor® 647 anti-his antibody (1:250, BioLegend, 362611). Sera/plasma samples were single or serial diluted (three-fold dilutions from 1:90 ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and a further 1 h incubation with anti-His HRP conjugate (652504; BioLegend, San Diego, CA, USA) diluted (1:20000 ...
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bioRxiv - Bioengineering 2023Quote: The antibodies used for flow cytometry were as follows: anti-His Tag APC (BioLegend, USA, Cat: #362605), anti-human IgG (G18-145 ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Immunology 2022Quote: ... prior to incubation with staining antibodies (PE/Cy7 anti-mouse CD45, BV711 anti-mouse CD3, BV421 anti-mouse CD19, FITC anti-mouse CD31, and APC anti-mouse podoplanin, all BioLegend) and one Hashtag antibody per replicate using BioLegend TotalSeq-B03 (catalog numbers 155831 ...
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bioRxiv - Cell Biology 2021Quote: ... purified mouse anti mouse/human CD207 (Biolegend, 144202), rabbit anti-mouse/human Ki67 (abcam ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal against mouse β-III-tubulin (Biolegend); mouse monoclonal against HA (12CA5 ...
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bioRxiv - Immunology 2022Quote: Mouse cytokine (LEGENDplex™ Mouse Inflammation Panel, Biolegend) and chemokine (LEGENDplex™ Mouse Proinflammatory Chemokine Panel ...
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bioRxiv - Immunology 2023Quote: ... AF700-conjugated mouse anti-mouse CD45.2 (104; BioLegend), PE-Cy5-conjugated mouse anti-mouse CD45.1 (A20 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
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bioRxiv - Microbiology 2022Quote: ... and AF700-conjugated mouse anti-mouse CD45.2 (104; BioLegend) along with eF780 viability dye (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... PE-Cy5-conjugated mouse anti-mouse NK1.1 (PK136; BioLegend), PE-Cy5.5-conjugated rat anti-mouse CD4 (RM4-5 ...
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bioRxiv - Immunology 2024Quote: ... mouse anti-mouse amyloid beta (Clone 6E10, Biolegend, 803004), and rabbit anti-mouse Iba1 (Fujifilm ...
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bioRxiv - Immunology 2023Quote: ... we cultured mouse T cells in mouse RP10 with recombinant mouse TGF-β1 (Biolegend Cat # 763104), rIL-12 ...
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bioRxiv - Immunology 2021Quote: ... Bound ligand was detected with a horseradish peroxidase (HRP)-conjugated anti-His antibody (652504, BioLegend, San Diego, CA, USA). After incubation for 1 hour at room temperature and washing ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a further 1 h incubation with anti-His HRP conjugate (BioLegend; San Diego, CA, USA; cat. no. 652504) diluted (1:2500 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Immunology 2023Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
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bioRxiv - Biochemistry 2020Quote: ... and isotype controls (mouse IgG-FITC, mouse IgG2a-APC; BioLegend). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse mIgD PE/CY7 and mouse IFNγ APC/CY7 (Biolegend). FVD506 and 7AAD were used to distinguish viable cells ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-human/mouse Grazyme B-PE Cyanine 7 (BioLegend), rat anti-mouse CD8-FITC (BioLegend) ...
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bioRxiv - Cell Biology 2020Quote: ... HA (mouse; BioLegend), TGN46 (sheep polyclonal ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse TGFβ1 (Biolegend), N-acetyl cysteine (NAC ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Immunology 2021Quote: ... anti-mouse mouse MHC H-2 hashtag antibodies (Biolegend TotalSeq™) were used to label separate FACS-purified subsets which were washed ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal α-GFP and/or mouse monoclonal α-RpoA (BioLegend) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... and mouse recombinant PE anti-human/mouse TLR2 (clone QA16A01; Biolegend) were used.
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bioRxiv - Immunology 2021Quote: ... LEGENDPlex assays (Mouse Proinflammatory Chemokine Panel and Mouse Inflammation Panel; BioLegend) were used ...
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bioRxiv - Cancer Biology 2022Quote: ... a mouse biotinilated anti-mouse CD45 antibody (clone 30-F11, Biolegend) was used ...
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bioRxiv - Immunology 2024Quote: ... into the mouse tail vein (5µg/25g mouse, Clone 1A8, Biolegend). 48 hours after the first FITC anti-mouse Ly-6G antibody injection ...
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...