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Citations for BioLegend :
351 - 400 of 645 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and then incubated overnight at 4 °C with primary antibodies directed against amino acids 6-18 (Tau 12, 1:1,000 dilution, ref: 806501, Biolegend), 159–163 (Tau HT7 ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... Primary incubation was done overnight at 4 °C with rabbit anti-CKAP2 1:2000 or mouse anti-GAPDH (BioLegend, 649201 ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Genomics 2024Quote: ... The sorted cells were centrifuged at 400xg for 5mins at 4°C and re-suspended in 25μl Cell Staining Buffer (BioLegend). 2.5μl of 1:10 TruStain FcX™ PLUS (antimouse CD16/32 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell suspensions were stained with anti-SM-C/2.6 antibody (Fukada et al., 2004) as well as anti-CD45-PE (diluted 1/500, Biolegend), anti-CD31-PE (diluted 1/500 ...
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bioRxiv - Microbiology 2020Quote: ... Cleared lysates were transferred to new tubes and incubated overnight at 4°C with anti-GFP Nanobody Affinity gel (BioLegend). The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... DC were stained with PE CD45 (30-F11) and APC CD11c (N418) for 25 minutes at 4°C (all Biolegend). Excess antibodies were removed by washing with HBSS + 0.1% BSA ...
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bioRxiv - Immunology 2021Quote: ... Amplified antibody barcodes were recovered from the supernatant and were processed to generate TotalSeq-C libraries as instructed by the manufacturers (10X Genomics and BioLegend, TotalSeq-C with 10x Feature Barcoding Protocol) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Immunology 2021Quote: ... The sorted fraction was sequentially stained for 20 min at 4 °C with the Ly51-PE antibody (1:800) (Biolegend) and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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bioRxiv - Immunology 2021Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer or peptide probes preincubated with streptavidin (30 minutes at molar ratio of 1:4 at 25°C) conjugated to PE (405203, BioLegend), BV421 (405226 ...
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bioRxiv - Genomics 2020Quote: ... loss of MHC class I expression in B2M knock-outs was assessed by staining with FITC-conjugated anti-HLA-A,B,C (W6/32, Biolegend). To maximize surface expression of IFNγ regulated proteins ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were collected after 45 min incubation at 37 °C and 5 % CO2 and stained with antibodies to microglia cell surface markers (CD11b-PE-Cy7, 1:200, Biolegend, #101216 ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were fixed for 15 min at 37°C after surface marker and viability dye staining using Fixation Buffer (Biolegend), followed by permeabilization for 1h at −20°C with True-Phos™ Perm Buffer (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... Primary antibody labeling was performed in blocking buffer overnight at 4°C in a humidified chamber using the following antibodies: anti-CD4 Alexa Fluor 647 (BioLegend), goat anti-IgD purified (Cedarlane Labs) ...
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bioRxiv - Immunology 2021Quote: ... 2×106 single splenocytes or BAL cells were incubated for 4 hours at 37° C in the presence of PMA/Ionomycin and Brefeldin A (Cell Activation Cocktail; BioLegend) or GolgiPlug control (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Systems Biology 2021Quote: ... A maximum of 1×106 cells/donor were stained by oligo-barcoded antibodies (TotalSeq-A or TotalSeq-C; Biolegend, Expedeon) for 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with brefeldin A 2h at 37°C before surface stain with a mix of CD107a (LAMP1) [clone 1D4B (BioLegend)] and CD107b (Mac-3 ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...
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bioRxiv - Immunology 2022Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Molecular Biology 2020Quote: ... MNCs were harvested by ficoll gradient centrifugation and human hematopoietic cells were identified by flow cytometry using the following antibody cocktail: HLA-A/B/C FITC (clone W6/32, Biolegend), mouse CD45.1 PE-CY7 (clone A20 ...
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...
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bioRxiv - Immunology 2019Quote: Macrophages were stained for 40 min at 4°C with fluorophore-conjugated mAb FITC-anti-CD36 (clone 5-271, Biolegend), or PE-anti-CD209 (clone 120507 ...
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bioRxiv - Microbiology 2021Quote: T cells were washed and incubated with antibodies (described above) at 4°C for surface staining and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2019Quote: ... and incubated for 1 h at 4°C in the dark: CD3 (anti-CD3-APC-780; clone 145-2C11, Biolegend), CD4 (anti-CD4-PE-Cy7 ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...