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Citations for BioLegend :
401 - 450 of 6119 citations for Bcl 2 Binding Component 3 BBC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728, 1:1000), F4/80 (Biolegend Cat # 123110 ...
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bioRxiv - Immunology 2020Quote: ... Cells were surface stained and washed with FACS buffer then resuspended in 100 μL Annexin V binding buffer (Biolegend: 79998). Cells were then incubated with 5 μL APC Annexin V (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... and NMHC-IIB antibody (#909901) binding to residues 1965-1976 in the C terminus (1:100 IF, 1:400 WB) were purchased from BioLegend. Rabbit polyclonal antibody against GFP tag (#50430-2-AP ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were blocked for non-specific binding using the cell staining buffer and then were fixed and permeabilized using True Phos Perm Buffer (BioLegend, 425401) before antibody staining ...
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bioRxiv - Physiology 2023Quote: ... Cisplatin is washed off and non-specific binding was blocked with a 10-minute staining of cells with Fc Block (Biolegend; 1µg). Cells were stained with 0.5µg of cell surface metal-conjugated antibodies for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b+CD45.2+CX3CR1+ microglia were sorted into a pre-cooled 5ml low-protein binding falcon tube (fisher scientific; Cat#: 13-864-407) containing 0.5ml cell staining buffer (BioLegend, Cat#: 420201). Walls of the tubes were washed and cells pelleted at 300g ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Galectin-3-AF647 (125408) from BioLegend and anti-CHMP4B (13683-1-AP ...
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bioRxiv - Immunology 2022Quote: ... and 3 μg/ml anti-CD28 (Biolegend) in RPMI with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... and rTGFβ (3 ng/ml; Biolegend, #580706), with or without 2.5μM mCRAMP ...
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bioRxiv - Immunology 2020Quote: ... and rTGFβ (3 ng/ml; Biolegend, #580706), with or without 2.5 μM mCRAMP for 1 to 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... β3-tubulin (Biolegend #801201, dilution 1:1000), cleaved caspase-3 (Novus Biologicals #MAB835 ...
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bioRxiv - Immunology 2021Quote: ... [clone 1D4B (BioLegend)] and CD107b (Mac-3) [clone M384 (BioLegend)].
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bioRxiv - Genomics 2022Quote: ... CD55 (PE-conjugated; clone RIKO-3; Biolegend), CD41 (APC-conjugated ...
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bioRxiv - Immunology 2022Quote: ... FITC-TIM-3 (clone F38-2E2, BioLegend), FITC-LAG-3 (clone 11C3C65 ...
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bioRxiv - Immunology 2022Quote: ... and anti-mouse LAG-3 (C9B7W, Biolegend), which were stained for 60 min at 37°C as previously described (Gagliani et al. ...
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bioRxiv - Immunology 2023Quote: ... IgG2b BV605 or R718 (R12-3, BioLegend), IL-7Rα BV605 or BV421 (AFR34 ...
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bioRxiv - Immunology 2023Quote: ... Panel 3) CD3-AF700 (Biolegend Cat. 300323), CD4-APC Fire750 (Biolegend Cat ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Galectin-3 (1:1000; Biolegend, 125401), anti-γ-H2AX (1:200 ...
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bioRxiv - Immunology 2024Quote: ... anti TIM-3-BV605 (RMT3-23, Biolegend), anti CD44-PE-Cy7 (IM7 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend), CD127 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... catalog number L100-L115-01) in PBS with 1% BSA and incubated the beads with 3.5 µg/ml biotinylated anti-CD81 antibody (BioLegend, clone Eat-2) at the room temperature for 40 minutes ...
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bioRxiv - Immunology 2019Quote: ... according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 μg/mL anti-human RP105 antibody clone MHR73-11 (BioLegend Cat.# 312907).
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation (1500rpm; 5mins; 4°C) and washed twice with Annexin V binding buffer (Biolegend; 1ml per wash). The cell pellets were resuspended in of binding buffer (100μl ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...