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Citations for BioLegend :
201 - 250 of 4347 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... For antibody validation we incubated 1mio cells in 100µl Cell Staining Buffer (BioLegend; Cat# 420201) and added 5µl Human TrueStain FcX™ and 0.5µl TruStain FcX™ PLUS ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... For antibody validation we incubated 1mio cells in 100µl Cell Staining Buffer (BioLegend; Cat# 420201) and added 5µl Human TrueStain FcX™ and 0.5µl TruStain FcX™ PLUS ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellet was resuspended in red blood cell lysis buffer (BioLegend, San Diego, CA, USA) for 90 seconds and then diluted in 3x volume of PBS ...
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bioRxiv - Bioengineering 2022Quote: ... All the cells were stained for live/dead cells using ZombieAqua™ viability dye (Biolegend) at 1:100 dilution ...
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bioRxiv - Immunology 2019Quote: ... Isolated T cells were then stained using CFSE Cell Division Tracker Kit (Biolegend Catalog # 23801). CFSE stained T cells were then collected and distributed into round bottom 96 well plates at 100,000 cells per well in IL-2(30 IU ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-activated before membrane staining using Cell Activation Cocktail (with Brefeldin A, Biolegend) for 2 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: Human ILC2 cells were stained with 1 μM CSFE (CSFE cell division tracker kit, BioLegend) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Lung cells were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Immunology 2022Quote: ... 5×105 SABR expressing NFAT-GFP-Jurkat cells were labeled with cell trace violet (BioLegend) according to the manufacturer’s protocol before incubation with 5×105 TCR expressing Jurkat cells in a round bottom 96-well plate for 16-20 hr ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained by incubating cell suspensions with Zombie Aqua (BioLegend, San Diego, CA) diluted at 1:1000 dilution in PBS for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Tfr cells were resuspended at 20 × 106 cells/ml in CITE-seq staining buffer (BioLegend) and incubated with Human TruStain FcX Fc Blocking reagent for 10 min at 4°C to block nonspecific antibody binding ...
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bioRxiv - Immunology 2023Quote: ... Viable cells were identified using Zombie Green cell viability stain (BioLegend, San Diego, CA, USA). See Sup ...
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bioRxiv - Cancer Biology 2023Quote: E0771 or E0771hPD-L1 cells were washed twice with ice-cold cell staining buffer (BioLegend) and stained with IgG control ...
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bioRxiv - Biochemistry 2023Quote: ... The activation state of T cells was confirmed with increased cell size and CD44 (BioLegend) expression by FACS ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated using MojoSort Mouse CD8 T Cell Isolation Kit (480035, BioLegend). CD8+ T cells were cultured and expanded in a similar fashion to γδ T cell populations ...
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bioRxiv - Immunology 2023Quote: ... red blood cells (RBC) were lysed by incubating cells with RBC Lysis Buffer (Biolegend #420301) for 3 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for cell surface markers as follows: APC/Cy7 anti-CD3 (BioLegend #300318), AF700 anti-CD4 (BioLegend #300526) ...
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bioRxiv - Immunology 2023Quote: Cells were stained with Carboxyfluorescein diacetate succinimidyl ester (CFSE) Cell Division Tracking Kit (BioLegend, 423801), and then cultured in complete RPMI1640 with IL-2 for 3 days ...
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bioRxiv - Immunology 2023Quote: The resulting cell isolates were treated with 10ml red blood cell (RBC) lysis buffer (BioLegend). The cells were resuspended in PBS with 1% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... harvested cells were incubated in RPMI with a cell activation cocktail with brefeldin A (BioLegend) for 4 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained with a cocktail of cell surface antibodies (Biolegend, Table 2) in 0.5%BSA/PBS and analyzed on a LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Cells requiring intracellular staining (our T cell panel) were washed with permeabilization buffer (Biolegend, 421002) after surface staining and prior to fixation ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were purified with the MojoSortTM mouse CD4 T cell isolation kit (Biolegend, 480006) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed in PBS and stained with 2.5 Tag-it Violet cell tracker (Biolegend) as previously described.
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bioRxiv - Immunology 2024Quote: ... 2C CD8 T cells were stained with 2.5 uM cell tracker Tag-it Violet (Biolegend) in the same manner as 2cz hybridoma.
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bioRxiv - Immunology 2024Quote: ... cells were first stimulated with cell activation cocktail (1:500 or 1:2500) (Biolegend, 423301), BFA (5 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: 1×106 epithelial target cells were washed and re-suspended in the cell staining buffer (Biolegend) containing different dye-conjugated primary antibodies at 1 μg/ml or 5 μg/ml for 30 minutes at 4°C ...
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bioRxiv - Genomics 2019Quote: ... cells were stained in batches of 1×107 cells for 30 min at 4°C (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Genomics 2020Quote: ... and the cell pellet was resuspended to 108 cells/mL in 1X Nanobead buffer (BioLegend #480017). Contaminating immune cells were depleted using anti-CD45 Magnetic Nanobeads (BioLegend #488028 ...
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bioRxiv - Bioengineering 2021Quote: ... the positive target cells (Raji) and negative target cells (K562) were labeled with Calcein-AM (Biolegend) and then cocultured with effector cells (CAR19 and sCAR19 cells ...
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bioRxiv - Immunology 2021Quote: ... cells were purified using negative magnetic bead selection (MojoSort Mouse Pan Dendritic Cell Isolation Kit, BioLegend). 2.5×106 BMDCs per well were cultured for 6h in 6-well plates in RPMI complete (R10 ...
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bioRxiv - Genomics 2019Quote: ... CD19-positive cells were removed (StemCell) and CD4+ T cells were enriched with magnetic beads (Biolegend) prior to sorting naïve T cells (CD4+CD45RO- ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... Fixed cells (106 cells/sample) were permeabilized using Intracellular Staining Permeabilization Wash buffer (BioLegend, cat.421002) according to the manufacturer instructions and stained with primary mouse monoclonal antibodies for SQSTM1/p62 (Abcam ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... cells were stained with 10 µl per 10 million cells of CD326-AlexaFluor647 antibody (Biolegend, 118212), CD31-APC (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Proliferation of CD8+ T cells was determined using the CFSE Cell Division Tracker Kit (Biolegend 423801) according to the manufacturer’s protocols.
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Interleukin 4 controls the role of macrophages in pulmonary metastatic tumor cell seeding and growthbioRxiv - Cancer Biology 2021Quote: ... Single cell suspensions were incubated for 10 min in red blood cell (RBC) lysis buffer (Biolegend) on ice to eliminate erythrocytes ...
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bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
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bioRxiv - Immunology 2022Quote: Mouse pre-stained cells or spleen cells were washed once and incubated with TruStain FcR (BioLegend), True-Stain Monocyte Blocker (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were first depleted of CD45+ cells with MojoSort mouse CD45 nanobeads (BioLegend #480028) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... CD19-positive cells were removed (StemCell) and CD4+ T cells were enriched with magnetic beads (Biolegend) prior to sorting Tfh cells (CD4+CD25- CXCR5hiPD1hi) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were either enriched for γδ T cells using Mojo Sort magnetic beads (BioLegend) for depletion of CD4 and CD8 T cells or sorter for Vγ4 T cells ...
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bioRxiv - Molecular Biology 2023Quote: Cells were washed in PBS and stained with Zombie UV® Fixable Dead Cell Stain (Biolegend), and rinsed once with PBS and blocked in blocking buffer (PBS with 50% FCS ...
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bioRxiv - Immunology 2023Quote: ... cells were enriched from total splenocytes using the EasySep Mouse CD8+ T cell Isolation Kit (Biolegend) at day 3.5 post infection ...
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bioRxiv - Microbiology 2023Quote: ... The resulting adherent cells were detached from the petri dishes using Accutase cell detachment solution (Biolegend) and seeded into 12 or 24 well plates (Corning ...
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bioRxiv - Immunology 2023Quote: ... The cells were first washed using pre-chilled Cell Staining Buffer (Biolegend, San Diego, CA, USA) supplemented with 2mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... cell-surface stained cells were fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (BioLegend), incubated with anti-CD5L antibody for 30 min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... cells were trypsinized and single cells were stained with the respective CD47 (BioLegend Cat. No. 323124), IFNGR1 (also known as CD119 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were selected by repetitive cell sorting using a validated monoclonal antibody (Clone T2.5, Biolegend 121810) until a homogenous polyclonal negative population was obtained ...
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bioRxiv - Immunology 2023Quote: ... Sorted γδ T cells were stained with 2.5 nM CFSE Cell Division Tracker Kit (423801, BioLegend) for 20 min at 37 °C immediately after sorting ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed twice in Cell Staining Buffer and fixed with Fixation Buffer (Biolegend 420801) for 30 min at 4°C ...