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Citations for BioLegend :
51 - 96 of 96 citations for 8 Cyano octanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and collected data analysed and presented by FlowJo V.10.5.0 (FlowJo LLC, OR, USA, www.FlowJo.com) 8-Peaks Rainbow Calibration Particles (Biolegend #422903) was used for routine alignment and performance verification of the flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... 8-10 week old C57BL6/J mice received either 200ng of IFNα (Biolegend 752802, ∼ 1×104 U) or saline intranasally (N = 2) ...
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bioRxiv - Genomics 2022Quote: ... cells were activated at ∼2-4M cells/ml for 8 hrs using CD3 antibody (Biolegend clone OKT3) coated tissue culture plates ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: Antibodies and clones reactive against the following proteins were used: CD2 (TS1/8, BV421 or Pacific Blue, Biolegend), CD3 (UCHT1 ...
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bioRxiv - Developmental Biology 2022Quote: ... HTO staining was carried out with 0.5μg of β2M and CD298 antibody-conjugated oligonucleotides (Total-seq B Hastags 4, 6 and 8; BioLegend) with ∼500,000-750,000 isolated single cells ...
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bioRxiv - Neuroscience 2022Quote: ... room temperature incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421TM Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2019Quote: ... RT incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421™ Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
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bioRxiv - Bioengineering 2021Quote: ... FITC anti-mouse H-2Kb/H-2Db Antibody (Clone 26-8-6) were from Biolegend (San Diego, CA). Primary antibodies of STING/TM173 (D2P2F) ...
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bioRxiv - Genetics 2022Quote: ... cKit-BV510 (104D2) and NKp44-Alexa Fluor (AF)647 (P44-8) all purchased from Biolegend (San Diego, CA), CRTH2-PE (301109 ...
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bioRxiv - Immunology 2022Quote: ... and two hashtag antibodies were built by combining the antibodies MHC I-Biotin (Biolegend, clone 28-8-6) and CD45-Biotin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... injections of a TH17 priming cocktail (n=8; 0.5μg rmIL-6 [575704, BioLegend, USA] and 1μg rmTGF-β1 [763104, BioLegend, USA]) or rest (n=8 ...
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... cell free supernatant was collected after 24 hours and IL-8 was quantified by ELISA according to manufacturer’s instructions (BioLegend).
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bioRxiv - Bioengineering 2022Quote: ... the streptavidin modified nanovial solution was then mixed with a solution containing 8 μg/mL anti-human CD45 (Biolegend) at equal volume ...
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bioRxiv - Systems Biology 2022Quote: ... Each tube was made up to 100μL with cell staining buffer and TotalSeq Hashtag (HTO 1-8) reagents (BioLegend) were added to each sample for 20min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... for 8 h at 37 °C followed by staining with a fixation/permeabilization buffer according to the manufacturer’s protocol (BioLegend). After washing ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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bioRxiv - Immunology 2023Quote: ... each sample was labelled with 0.1 ug of a cell hashing antibody (TotalSeq-B anti-human Hashtag antibodies 1 through 8, BioLegend). Cells were labelled for 30 minutes on ice in the dark ...
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bioRxiv - Immunology 2022Quote: ... and 1X nonessential amino acids and stimulated with plate-bound αCD3 mAb (clone HIT3a, BioLegend, cat#300313) at 1 μg/mL and soluble αCD28 mAb (CD28.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Immunology 2019Quote: ... glass slides or 8 well chambers were coated with either ICAM-1 alone or anti-CD3 (clone Hit3a from Biolegend)/anti-CD28 (clone CD28.2 from Biolegend)/ICAM-1 (R&D systems ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Immunology 2020Quote: ... for 8 hours and then surface staining was performed using the following abs anti-human BDCA-2-PE (clone AC144; Biolegend), CD123-PeCy7 (clone 6H6 ...
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bioRxiv - Immunology 2022Quote: The presence of IL-17A in T-cell culture supernatants and of IL-6 and IL-8 in fibroblast culture supernatants was quantified by ELISA according to manufacturer instructions (deluxe or standard kits, respectively, all BioLegend). Plates were read at 450 nm using a Spark 10M (Tecan) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3–8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3-8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Machine calibration was performed by flow cytometry facility staff before each sort using Sphero™ 8-peak rainbow calibration beads (BioLegend) to achieve optimal stream alignment ...
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bioRxiv - Neuroscience 2021Quote: ... Measurements of cytokine secretion were made 8 and 24 hrs after activation in the presence of BSA (0.4 μM) using Max deluxe (Biolegend, CA, USA) ELISA kits [24] ...
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bioRxiv - Cancer Biology 2019Quote: ... Circulating leukemia cells were detected on day 8 by flow cytometry using a human-specific CD45 antibody (#404012, Biolegend, San Diego). Mice were sacrificed upon signs of morbidity.
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bioRxiv - Immunology 2022Quote: ... T cells at 1×106/ml were kept in culture withdraw of cytokines for 8 hours before stimulated with 1µg/ml plate-coated anti-mouse CD3e (Biolegend, Cat.100340) and 1µg/ml soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated in primary antibodies diluted in iFLASH blocking buffer for 3 days (anti-keratin 8 primary antibody 1:100 [Hybridoma Bank, TROMA-1]; anti-keratin 14 primary antibody 1:100 [Biolegend, 905301]). Tissues were washed 3×1h with PBT at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated in 2% BSA in PBS overnight at +4°C (anti-keratin 8 primary antibody 1:1000 [Hybridoma Bank, TROMA-1]; anti-keratin 14 primary antibody 1:1000 [Biolegend, 905301]). Then ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2023Quote: To determine galectin-3 or galectin-8 expression the following: intracellular protein and lysosomes were stained using anti-Lamp1-BV421 (clone H4A3, BioLegend, USA), anti-Galectin-3-AF488 (clone B2C10 ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated overnight at 4 °C with primary antibodies directed against amino acids 6-18 (Tau 12, 1:1,000 dilution, ref: 806501, Biolegend), 159–163 (Tau HT7 ...
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bioRxiv - Immunology 2021Quote: ... NK were co-cultured for 8 h with target cells in the presence of CD107a PerCP-Cy5.5 antibody (H4A3, BioLegend, 250 ng/ml) and 2.5 ng/ml IL-15 (R&D) ...
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from triplicate wells of each condition were collected at 24 hrs and assayed for the IL-8 concentration using a human IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend, San Diego, CA). IL-8 values were reported as normalized values defined as a proportion increased or decreased compared to values obtained for J99 ...
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bioRxiv - Biophysics 2019Quote: ... anti-CCR5 (Clone T21/8) directly coupled to PE was from eBioscience (San Diego, CA) and anti-Flag PE from Biolegend (San Diego, CA). Dulbecco’s modified Eagle’s medium Glutamax (DMEM-Q) ...
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bioRxiv - Immunology 2022Quote: ... Circulating maternal neutrophils were depleted at days 5 (E4.5) and 8 (E7.5) of pregnancy using a monoclonal neutralizing antibody (Biolegend, clone 1A8; 50 μg i.v.) or isotype control (Biolegend clone RTK2758 ...
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bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...