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Citations for BioLegend :
251 - 300 of 6996 citations for 8 CHLORO 2 METHYL IMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and BCL-2-Alexa Fluor 647 (Biolegend) for 3 hours ...
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bioRxiv - Bioengineering 2023Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; BioLegend) was diluted 1:10,000 from a 5 mg mL-1 stock solution in deionized water ...
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bioRxiv - Microbiology 2023Quote: ... IL-2-APC (MQ1-17H12 clone, Biolegend), TNFα-AF700 (Mab11 clone ...
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bioRxiv - Immunology 2023Quote: ... CD49d (clone R1-2, BioLegend, catalog #103618).
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bioRxiv - Immunology 2024Quote: ... PE-CD32a/b (FUN-2, Biolegend #303206), PE-CD64 (10.1 ...
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bioRxiv - Immunology 2024Quote: ... or CD18-activating (clone M18/2, Biolegend) antibodies were added to the solution at 1/50 dilution.
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bioRxiv - Immunology 2024Quote: ... and hCD206 (BioLegend, #321136, clone 15-2). Dead cells were stained with 2 μg/ml propidium iodide (Sigma ...
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bioRxiv - Immunology 2024Quote: ... CD18-activating antibody (clone M18/2, Biolegend), CD18-blocking antibody (clone GAME-46 ...
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bioRxiv - Immunology 2023Quote: ... anti-human CD206 (clone 15-2, BioLegend), anti-human CD80 (clone W17149D ...
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bioRxiv - Immunology 2024Quote: ... and CD206 (MRC1)-FITC (15-2; BioLegend). After 20 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... and IL-15 [2 ng/ml] (BioLegend), media change 3 times per week and culture splitting 1 time per week ...
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bioRxiv - Immunology 2024Quote: ... IL-2: BV605 (Biolegend, Clone: JES6-5H4), TNFα (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM EDTA then stained with anti-mouse CD31-APC (1:500, Biolegend, 102507), CD45-APC (1:500 ...
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bioRxiv - Immunology 2021Quote: ... at 1:100 dilution in 2% BSA-PBS prior to using streptavidin BV421 (405225, BioLegend) and anti-human VRC01 mAb conjugated to AF488.
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bioRxiv - Neuroscience 2021Quote: ... and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL, VCAM-1, 429 MVCAM.A, BioLegend) were used ...
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bioRxiv - Immunology 2023Quote: ... hydrogel beads were stained with anti-CD138 PE (1:80 dilution, clone 281-2, BioLegend), anti-FLAG PE-Cy7 (1:200 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Western blot analysis of the samples were done using anti- HA (1:2 000; BioLegend) and anti-Flag M2 (1:5 000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were stained for CD69 (clone FN50, BioLegend, dilution 1:500 in PBS + 2% FBS) expression after 4 - 24 hr at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... and different concentrations of Tregs (1:2-1:64 Treg: Teff ratio, 500-2000 Tregs) were activated with 2μg/ml anti-CD3 (Biolegend) in a 96-well round bottom plate with 100ul RPMI for 72hrs (Turnis et al. ...
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bioRxiv - Genomics 2024Quote: ... at a ratio of 1:1 (cell:Dynabead) in the presence of 5ng/mL rhIL-2 and 10ng/mL rhIL-7 (Biolegend) in 96 well U-bottom plates ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-keratin 8 (BioLegend, 904801; 1:200), and mouse monoclonal anti-β-casein (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-Keratin 8 antibody (BioLegend, 1:500) was performed after heat-induced antigen retrieval ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended at 2×106 in 100μl PBS with 2% FBS and incubated with Zombie violet fixable viability dye (Biolegend) and an antibody cocktail for 25min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... Trypsinized tumor cells were counted and stained with carboxyfluorescein diacetate succinimidyl ester (CFSE, 1 μL/mL; BioLegend) following the manufacturer’s protocol and cultured overnight ...
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bioRxiv - Immunology 2019Quote: ... Carboxyfluorescein diacetate succinimidyl ester CFSE was purchased from Biolegend. MDP ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2×106 cells were incubated with mouse anti-HLA class I antibody (1:200, BioLegend) for 30min followed by secondary antibody incubation for 30min ...
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Immunology 2023Quote: ... for 5 min on ice before staining with fluorochrome-conjugated antibodies in FACS staining buffer (1× PBS, 2% FBS): CD3 (1:50 dilution; clone 145-2C11, BioLegend), CD8α (1:200 dilution ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes (2×105 cells/mL) were labeled with 5µM cell-permeant Calcein-AM in DMSO (1:200, BioLegend, 425201) for 20 minutes at 37°C ...