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Citations for BioLegend :
101 - 150 of 8216 citations for 7 chloro 1 2 diethylamino ethyl 5 2 fluorophenyl 1 3 dihydro 2H benzo 1 4 diazepin 2 one monohydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL (1 µg) of the corresponding hashing antibody (Totalseq-A, Biolegend, Table S4) were added to the blocked samples ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Physiology 2022Quote: ... Samples were stained with 3 μM of (4’,6-Diamidino-2-Phenylindole, Dilactate (DAPI, Biolegend Cat# 422801)) ...
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bioRxiv - Immunology 2019Quote: ... or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate; Biolegend). Intracellular staining was carried out using Human FoxP3 Buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Immunology 2023Quote: ... for 5 min on ice before staining with fluorochrome-conjugated antibodies in FACS staining buffer (1× PBS, 2% FBS): CD3 (1:50 dilution; clone 145-2C11, BioLegend), CD8α (1:200 dilution ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes (2×105 cells/mL) were labeled with 5µM cell-permeant Calcein-AM in DMSO (1:200, BioLegend, 425201) for 20 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were washed with FACS buffer (PBS containing 2% fetal calf serum and 1 mM EDTA) and 1 µL of FcX TruStain PLUS (Biolegend) was added to each sample ...
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Genetics 2020Quote: ... 7-aminoactinomycin D (7-AAD) (1:100; Biolegend, 420404) was added as a viability stain ...
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bioRxiv - Immunology 2019Quote: ... They were blocked for 1 hour at room temperature in 2% donkey serum and CD16/CD32 (Biolegend, Clone 93, 1:200). Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF771 goat anti-IL-1R ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM EDTA then stained with anti-mouse CD31-APC (1:500, Biolegend, 102507), CD45-APC (1:500 ...
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bioRxiv - Immunology 2021Quote: ... at 1:100 dilution in 2% BSA-PBS prior to using streptavidin BV421 (405225, BioLegend) and anti-human VRC01 mAb conjugated to AF488.
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bioRxiv - Neuroscience 2021Quote: ... and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL, VCAM-1, 429 MVCAM.A, BioLegend) were used ...
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bioRxiv - Immunology 2023Quote: ... hydrogel beads were stained with anti-CD138 PE (1:80 dilution, clone 281-2, BioLegend), anti-FLAG PE-Cy7 (1:200 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Western blot analysis of the samples were done using anti- HA (1:2 000; BioLegend) and anti-Flag M2 (1:5 000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were stained for CD69 (clone FN50, BioLegend, dilution 1:500 in PBS + 2% FBS) expression after 4 - 24 hr at 37 °C ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Immunology 2020Quote: ... 7-AAD (#420404, 1:100, BioLegend), Annexin V (#640947 ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2021Quote: ... 0.1% 2-mercapto-ethanol 1000X solution) supplemented with IL-7 (10 ng/mL, Biolegend). To activate T cells ...
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Immunology 2024Quote: ... Brefeldin A (5 μg/ml) and Monensin (2 μM) (BioLegend) were added to the cultures during the last 4 h of incubation to allow intracellular cytokine accumulation ...
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bioRxiv - Immunology 2019Quote: ... Antibodies include mouse anti-human ataxin-3 65042 1H9-2 (BioLegend), mouse anti-human MIC60 ab110329 (Abcam) ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2×106 cells were incubated with mouse anti-HLA class I antibody (1:200, BioLegend) for 30min followed by secondary antibody incubation for 30min ...
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend), CD127 ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Cancer Biology 2023Quote: ... CD51-PE (1:50, RMV-7, Biolegend), Sca1-PacificBlue (1:100 ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Cell Biology 2021Quote: ... mouse TYRP1 (MEL-5) (1:500; 4°C overnight; BioLegend, 917801), rabbit MITF (1:200 ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... IFN-β and IFN-γ were determined using the LEGENDplex mouse type 1/2 interferon panel (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Immunology 2020Quote: ... or 2) 10 μg/mL αLFA-1 + 10 μg/mL antibodies against CD16 (αCD16; BioLegend, clone 3G8). All loading solutions were mixed in PBS and supplemented with 10 μg/mL bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Cell Biology 2023Quote: ... 1–2 million cells/100 μl were incubated with the following antibodies: anti-CD11c PeCy7 (117318, Biolegend), anti-CD11b (101212 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend, 422302) in PBS ...