Labshake search
Citations for BioLegend :
251 - 300 of 366 citations for 7 Chloro 8 fluoroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Cells were stained with Alexa Fluor 647 anti-mouse Thy-1.1 Antibody (BioLegend; clone OX-7) and enrichment was confirmed with CytoFlex S (Beckman Coulter) ...
-
bioRxiv - Zoology 2020Quote: ... IL-8 and IL-12 were gotten from Biolegend (Camino Santa Fe, CA, USA). BCA protein quantitative kit and SYBR Green PCR kit were purchased from Smer Fell Science and Technology Co. ...
-
bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems ...
-
bioRxiv - Cell Biology 2023Quote: μ-side 8-well Ibidi chambers were coated with an anti-CD31 antibody (102502, BioLegend) for 30 min ...
-
bioRxiv - Immunology 2020Quote: ... re-suspended in PBS containing 0.5% BSA and 2mM EDTA and stained with 7-AAD (Biolegend, 420404) for 5’ at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... Secondary antibodies used in these studies against IL 10 (#501501) and IL-7 (#506601) were from Biolegend, and against IFN-γ (#MAB285) ...
-
bioRxiv - Neuroscience 2023Quote: ... Annexin: Apoptossi was assessed using PE Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, cat#: 640934). iMGL were collected and washed twice with Biolegend’s cell staining buffer ...
-
bioRxiv - Immunology 2023Quote: ... The cells were then stained with FITC Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend 640922) following the manufacturer’s instructions and subjected to flow cytometry ...
-
bioRxiv - Immunology 2023Quote: ... and plated in TRM-like culture media with 10 ng/mL mouse IL-7 (Biolegend Catalog No. 577804) and 10 ng/mL human TGFβ1 (Fisher Scientific PHG9214 ...
-
bioRxiv - Bioengineering 2023Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...
-
bioRxiv - Bioengineering 2022Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...
-
bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells (1x106) were stained with PE-labelled anti-human CD38 monoclonal antibody (clone HB-7, BioLegend Cat # 356604) in FACS buffer (0.5% FBS with 2 mM EDTA ...
-
bioRxiv - Cancer Biology 2023Quote: ... and PE anti-mouse H-2Kb/H2Db antibody (Clone 28-8-6; Biolegend; Cat #114607) on ice for 20 minutes in the dark ...
-
bioRxiv - Cell Biology 2021Quote: Detection of apoptotic cells was done by using FITC-Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend#640922). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were washed twice and resuspended in 300 μl of staining buffer containing 7-AAD (Biolegend, CA, USA) for flow cytometry analysis (Thermo Fisher Attune NxT - 14 color analyzer ...
-
bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using anti-human/mouse □7 integrin antibody (FIB504, Biolegend) and the EasySep Biotin Positive Selection Kit (Stemcell Technologies ...
-
bioRxiv - Immunology 2020Quote: ... Organs were processed as described in 32 and samples were stained with antibodies specific for Thy1.1 (clone OX-7, BioLegend), Thy1.2 (clone 30-H12 ...
-
bioRxiv - Immunology 2019Quote: 106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat ...
-
bioRxiv - Cancer Biology 2021Quote: Trypsinized and washed Panc02 cells were stained with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and analyzed using the CytoflexS Flow Cytometer (Beckman Coulter).
-
bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
-
bioRxiv - Microbiology 2022Quote: ... Apoptotic cells were detected using the PE Annexin V Apoptosis Detection Kit with 7-AAD according to the manufacturer’s protocol (BioLegend) and analyzed using an LSRII flow cytometer.
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
-
bioRxiv - Immunology 2023Quote: ... or 30 µL 1% FITC-Dextran70 (TdB Labs, 21024) to visualize vasculature and 7 µL APC-Ly6G (Biolegend, 127614) antibody to label neutrophil 30 min before imaging.
-
Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... Cells were then filtered through 35 μm nylon mesh and stained with Annexin V-FITC and 7-AAD (Biolegend). 50,000 cells per sample were collected for analysis using a LSRII flow cytometer (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... apoptosis of the Neuro-2a cell was analyzed by Annexin-V-FITC and 7-AAD staining accordingly to the manufacturer’s instruction (BioLegend). Cells were immediately acquired on a Canto II flow cytometer (BD Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
-
bioRxiv - Cancer Biology 2023Quote: ... MEFs were trypsinized and stained with Apotracker-Green (Apo-15 peptide) and 7-AAD according to manufacturer’s instructions (Biolegend) to detect apoptotic cells ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were labeled with antibodies detecting the following cell-surface markers: CD133-APC (Biolegend, clone 7, dilution 1:20), CD19-PECy7 (Biolegend ...
-
bioRxiv - Genomics 2024Quote: ... at a ratio of 1:1 (cell:Dynabead) in the presence of 5ng/mL rhIL-2 and 10ng/mL rhIL-7 (Biolegend) in 96 well U-bottom plates ...
-
bioRxiv - Immunology 2024Quote: ... purified ILC2 isolated from IL-33-challenged mice were cultured overnight with 10μM TOFA in complete media supplemented with 20 ng/ml IL-7 (Biolegend). Cells were washed with assay medium supplemented with 10 mM glucose ...
-
bioRxiv - Developmental Biology 2020Quote: ... Guinea pig anti-Cytokeratin 8+18 antibody and FITC rat anti-mouse/human CD45R/B220 (BioLegend) were used for fluorescence immuno-histochemistry ...
-
bioRxiv - Immunology 2020Quote: ... 8°C) and incubated for 10 min at 37°C and Zombie NIR Viability dye (Biolegend) was added to each well ...
-
bioRxiv - Immunology 2022Quote: ... and stained again with biotinylated anti-mouse/human TLR7/8 mAb and PE streptavidin (Biolegend, USA). To detect intracellular mouse IL-6 in splenocytes stimulated with various ligands in the presence of Brefeldin A (10μg/ml) ...
-
bioRxiv - Immunology 2021Quote: RAW264.7 cells of BMDMs were collected after washing in ice-cold PBS and then detaching the cells with Accutase (BioLegend). Prior to collection ...
-
bioRxiv - Immunology 2019Quote: ... and 40ng/ml IL-18) for day 1 intracellular cytokine staining (IL-12 from R&D, IL-7 and IL-15 from Biolegend).
-
bioRxiv - Bioengineering 2019Quote: ... MCF-7 cells in EPB were stained with a 1:20 dilution of FITC Anti-human CD326 (Biolegend, Cat.no. 324204) for 30 min at 4 °C to allow for population tracking in flow cytometry ...
-
bioRxiv - Immunology 2021Quote: ... processing omitted the 7-AAD step and incubated with a fixable live dead dye prior to incubation with fixation buffer (Biolegend) (15 mins) ...
-
bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... or biotin, 2G5, Beckman Coulter, or Ber-ACT8, Brilliant Violet 711, BUV395, BD Biosciences, or Ber-ACT8, PE-Cy-7, Biolegend), KIR2DL1 (FAB1844F ...
-
bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD71 expression in TF-1 and DAMI cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD71 (BioLegend) (#113811) ...
-
bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD11b expression MDS92 and MDS-L cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD11b (BioLegend) (#557743) ...
-
bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
-
bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and stimulated T cells with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used (mouseCD45-FITC from BD bioscience and 7-AAD, humanCD45-PE/Cy7, humanCD19-PE, humanCD3-APC and humanCD14-APC/Cy7 from Biolegend) and cells analyzed on the LSRFortessa flow cytometer (BD Biosciences) ...
-
bioRxiv - Cell Biology 2019Quote: ... 50µL of blood was stained for flow cytometry using conjugated antibodies (mouse CD45-FITC from BD bioscience and 7-AAD, humanCD45-PE/Cy7, humanCD19-PE, humanCD3-APC and humanCD14-APC/Cy7 from Biolegend) and analyzed on LSRFortessa flow cytometer (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
-
bioRxiv - Microbiology 2021Quote: ... Cells were then filtered using a 40 μm cell strainer and stained with the Live/Dead dye 7 Amino-actinomycin D (7AAD, Biolegend) and antibodies against CD45.2 (Biolegend ...
-
bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.