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Citations for BioLegend :
401 - 450 of 8208 citations for 7 Chloro 3 methyl 3H 1 2 3 triazolo 4 5 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Immunology 2019Quote: ... each well used for culture was incubated with 3 μg/mL anti-CD3 (Biolegend clone 145-2C11) and 3 μg/mL anti-CD28 (Biolegend clone 37.51 ...
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bioRxiv - Cancer Biology 2021Quote: ... and supplemented with murine SCF (10 ng/ml) and IL-3 (10 ng/ml) (Peprotech or BioLegend). The cells were serially passaged for ∼1 month ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with Alexa Fluor 647-anti-DYKDDDDK (BioLegend) at 0.5 μg/mL or with Alexa Fluor 488-streptavidin (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody diluted in 3% BSA (Alexa Fluor 594 Goat anti-Rat IgG, BioLegend, #405422) in the dark for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... one million cells were harvested 3 days after transduction and washed twice with cell staining buffer (Biolegend). Cells were resuspended in 100μL of Cell Staining Buffer (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... stained with 1:200 mouse IgG1 KRT18-647 clone DA-7 (BioLegend, 628404, lot. B208126) for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Developmental Biology 2021Quote: ... and treated with 7-AAD (BioLegend) (5uL per 100uL FACS buffer) ...
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bioRxiv - Immunology 2019Quote: ... and 1μg/mL 7-AAD (Biolegend) and stored at 4°C protected from light until acquisition ...
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bioRxiv - Microbiology 2021Quote: ... CD49a (TS2/7, Biolegend; PE-Cy7); PD-1 (EH12.2H7 ...
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bioRxiv - Cell Biology 2022Quote: ... 7-AAD (BioLegend, Cat no. 420404) was used as a viability marker ...
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bioRxiv - Immunology 2020Quote: ... and 1μg/mL 7-AAD (Biolegend) and stored at 4°C protected from light until acquisition ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD133-PE/Cy-7 (BioLegend) and UEA-FITC (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 7-AAD or Zombie Violet (Biolegend) was used to discriminate between live/dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 7-AAD Viability Staining Solution (BioLegend) was included in the sample preparation for flow cytometry to exclude dead cells from the analysis.
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... IL-7 and IL-23(BioLegend). CD56bright and CD56dim NK cells were expanded in NK MACS media(Miltenyi ...
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bioRxiv - Cancer Biology 2023Quote: ... and 7-AAD staining (Biolegend, 420404) via flow cytometry according to manufacturer instructions.
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bioRxiv - Immunology 2023Quote: ... CD169 (Biolegend, 7-239, PE-Cy7), and Sytox Green to identify monocyte and macrophage populations ...
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bioRxiv - Developmental Biology 2024Quote: ... 5uL of 7-AAD (BioLegend; 420404) and 5 μL Annexin V-APC (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Immunology 2023Quote: ... 0.5µg/mL) and ionomycin (1µg/mL) and for 3h at 37°C with brefaldin A (10µg/mL; Biolegend). Cell surfaces were stained ...
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bioRxiv - Immunology 2020Quote: ... CD81 (Eat-2; 1:1000; BioLegend), CD9 (EM-04 ...
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bioRxiv - Immunology 2022Quote: ... CD32 (FUN-2, BioLegend, 1:100), CD64 (10.1 ...
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bioRxiv - Immunology 2022Quote: ... CD32 (FUN-2, BioLegend, 1:100), CD64 (10.1 ...
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bioRxiv - Immunology 2022Quote: ... CD32 (FUN-2, BioLegend, 1:100), CD64 (10.1 ...
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bioRxiv - Immunology 2022Quote: ... CD32 (FUN-2, BioLegend, 1:100), and CD64 (10.1 ...
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bioRxiv - Cell Biology 2022Quote: ... and Keratin 5 (BioLegend #905501, 1:2000).
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bioRxiv - Cell Biology 2019Quote: ... cytokeratin 5 (K5) (1:200 BioLegend # 905501), cytokeratin 14 (K14 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-keratin 5 (Biolegend: 905501, 1:500) in 1% BSA / 4% serum overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... and CD4 (RM4-5) (Biolegend, 1:200). For quantification of in vitro infections ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5; BioLegend, dilution 1:200), CD25 (PC61 ...
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bioRxiv - Biochemistry 2020Quote: ... CD4 (Biolegend, cloneRM4-5, BV605, 1:100), CD8 (BD Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... CD-4 APC (1:200, rat, Biolegend, 100411), FoxP3-PE (1:20 ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (APC; 1:50; clone 11B11; BioLegend); IL-13 (PE ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2021Quote: ... The first surface marker panel included: BV421 and PerCP/Cy5.5 anti-TIM-3 (clones B8.2C12 and RMT3-23, BioLegend), BV510 anti-PD-1 (EH12.1) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...