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Citations for BioLegend :
451 - 500 of 6887 citations for 7 ACETOXY 2 CHLORO 1 HEPTENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
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bioRxiv - Immunology 2024Quote: ... plates were coated per well with 100 µL of anti-IgE capture antibody (2 µg/mL in PBS, clone RME-1, BioLegend) overnight at 4⁰ C ...
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bioRxiv - Immunology 2023Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...
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bioRxiv - Molecular Biology 2020Quote: ... together with the trypsinized cells were harvested into tubes and samples were prepared on ice for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... from BD Sciences (San Jose, CA); Alexa Fluor® 647-conjugated anti-rat Thy1 (OX-7, #202508; RRID:AB_492884) from BioLegend (San Diego, CA). Ghost Dye™ Violet 450 was from Tonbo Biosciences (#13-0863-T100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Developmental Biology 2023Quote: The flow cytometry staining for necrosis and apoptosis was was performed with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640922). Once the cells were incubated in 10 µM of EdU ...
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bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... anti-CD138 (clone 281-2, Biolegend). Specific conjugates are indicated within Figures ...
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bioRxiv - Immunology 2021Quote: ... and Thy1/2 (Biolegend, 30-H12) conjugated to APC-Cy7 each at a concentration of 0.25 μg per 1 x 106 cells to remove dendritic cells ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (20 ng/ml; BioLegend), IL-4 (20 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... IL-2 (clone: JES6-5H4; BioLegend) and IL-17A (clone ...
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bioRxiv - Immunology 2022Quote: ... and Monensin solution (2 μM) (BioLegend). Cells were then stained with one of four antibody panels (Supplementary Table 1 ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-2-PE-Cy7 (Biolegend) and anti-IL-4-PE (Biolegend) ...
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bioRxiv - Bioengineering 2021Quote: ... αCD4 mAb (clone RM4-2, Biolegend), anti-Camelid VHH antibody (clone 96A3F5 ...
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bioRxiv - Bioengineering 2021Quote: ... αCD4 mAb (clone RM4-2, Biolegend), αCD11b mAb (clone M1/70 ...
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bioRxiv - Immunology 2020Quote: ... α-BCL-2 (BCL/10C4, 633510, Biolegend), α-BIM (K.912.7 ...
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bioRxiv - Immunology 2020Quote: Panel 2: CD11b-BV421 (Biolegend, 301323), CD274-PE (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... anti-SDC1-PE (281-2, BioLegend), and anti-FR4-PE Cy7 (ebio12A5 ...
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bioRxiv - Immunology 2021Quote: ... IgD PE-Cy7 (IA6-2, BioLegend) Zombie NIR (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... IgD PE-Cy5 (IA6-2, BioLegend), CD14 PerCP (HCD14 ...
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bioRxiv - Immunology 2022Quote: ... IL-2 BV421 (Biolegend, cat.503826), IL-4 PE (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... α-CD8b.2 APC (53-5.8, BioLegend) and α-FOXO1 (C29H4 ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-2 and TNFα kits (BioLegend) and the experiments were done according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 200 IU/ml IL-2 (Biolegend), 12.5% FBS ...
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bioRxiv - Immunology 2023Quote: ... anti-CD49d (Biolegend, clone: R1-2), anti-NK1.1 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... IgD BV480 (Biolegend, clone IA6-2), CD5 BV785 (BD bioscience ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (clone JES6-5H4, BioLegend), TNF- α (clone MP6-XT22 ...
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bioRxiv - Immunology 2022Quote: ... IgD PC7 (Biolegend, clone IA6-2). Flow cytometry was performed as above with a BD Fortessa Analyzer and the FlowJo software.
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bioRxiv - Cancer Biology 2023Quote: ... and 1ng/mL IL-2 (BioLegend) for 72 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.5ng/mL IL-2 (BioLegend) for 72 hr at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... PE anti-IL-2 (Biolegend, 503807), BV510 anti-TNF-α (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... IFN-γ and IL-2 (Biolegend). Samples were analyzed by flow cytometry as described above.
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bioRxiv - Immunology 2023Quote: ... and 2 µM monensin (420701; Biolegend) were added to each well ...